Fish chemokines 14, 20 and 25: A comparative statement on computational analysis and mRNA regulation upon pathogenic infection

Arockiaraj, Jesu; Bhatt, Prasanth; Kumaresan, Venkatesh; Dhayanithi, Nagarajan Balachandran; Arshad, Aziz; Harikrishnan, Ramasamy; Arasu, Mariadhas Valan; Al-Dhabi, Naïf Abdullah

doi:10.1016/j.fsi.2015.09.015

Cited by 18 publications

(5 citation statements)

References 44 publications

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“…hydrophila, A. salmonicida, A. veronii [21,24,282,283,[286][287][288][289][290][291][292] Cytokines (TNF-α, TNF-β, IFN-γ, IL-6, IL-8, IL-10) [22,23] Epithelioma papulosum cyprini cell line (EPC); head kidney leukocytes (HKLs), human epithelial cell line (Hep-2); murine macrophage cell line (RAW 264.7); human epithelial type cell line (HeLa); human epithelial colorectal adenocarcinoma cell line (Caco-2); spleen leukocytes (SLs). The strains of these studies [282,283,286,[292][293][294]296,297,302] were identified by non-molecular methods or lack information about the identification.…”

Section: Fish Cells (Epc Hkls Hep-2)mentioning

confidence: 99%

“…A recent study by Kong et al [294] established that A. hydrophila might induce an overexpression of the pro-inflammatory cytokine gene TNF-α in the intestine of fish, deteriorating the integrity of the mucosal barrier structure. Similarly, the expression of different chemokines, which are a family of small cytokines with an important role in the immune response, has been studied after A. hydrophila infection in eel and grass carp [297,298].…”

Section: Fish Cells (Epc Hkls Hep-2)mentioning

confidence: 99%

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An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

2020

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The genus Aeromonas belongs to the Aeromonadaceae family and comprises a group of Gram-negative bacteria widely distributed in aquatic environments, with some species able to cause disease in humans, fish, and other aquatic animals. However, bacteria of this genus are isolated from many other habitats, environments, and food products. The taxonomy of this genus is complex when phenotypic identification methods are used because such methods might not correctly identify all the species. On the other hand, molecular methods have proven very reliable, such as using the sequences of concatenated housekeeping genes like gyrB and rpoD or comparing the genomes with the type strains using a genomic index, such as the average nucleotide identity (ANI) or in silico DNA–DNA hybridization (isDDH). So far, 36 species have been described in the genus Aeromonas of which at least 19 are considered emerging pathogens to humans, causing a broad spectrum of infections. Having said that, when classifying 1852 strains that have been reported in various recent clinical cases, 95.4% were identified as only four species: Aeromonas caviae (37.26%), Aeromonas dhakensis (23.49%), Aeromonas veronii (21.54%), and Aeromonas hydrophila (13.07%). Since aeromonads were first associated with human disease, gastroenteritis, bacteremia, and wound infections have dominated. The literature shows that the pathogenic potential of Aeromonas is considered multifactorial and the presence of several virulence factors allows these bacteria to adhere, invade, and destroy the host cells, overcoming the immune host response. Based on current information about the ecology, epidemiology, and pathogenicity of the genus Aeromonas, we should assume that the infections these bacteria produce will remain a great health problem in the future. The ubiquitous distribution of these bacteria and the increasing elderly population, to whom these bacteria are an opportunistic pathogen, will facilitate this problem. In addition, using data from outbreak studies, it has been recognized that in cases of diarrhea, the infective dose of Aeromonas is relatively low. These poorly known bacteria should therefore be considered similarly as enteropathogens like Salmonella and Campylobacter.

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Section: Fish Cells (Epc Hkls Hep-2)mentioning

confidence: 99%

Section: Fish Cells (Epc Hkls Hep-2)mentioning

confidence: 99%

An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

2020

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“…Recently, Danio rerio , has been used as a model organism to study diuron and diazinon induced toxicity (Velki et al, 2019). The whole genome sequence information and richness of bioinformatics data on the genetic characterization of Zebra fish makes it an ideal organism for analyzing pesticide toxicity and analysis of marker enzymes (Acrockiaraj et al, 2015a, Acrockiaraj et al, 2015b, Palanisamy et al, 2015). Due to synthetic pesticide toxicity variations in the activity of enzymes were reported previously in various non targeted organisms, including fishes.…”

Section: Introductionmentioning

confidence: 99%

Sub-lethal effect of synthetic pyrethroid pesticide on metabolic enzymes and protein profile of non-target Zebra fish, Danio rerio

Al‐Ghanim

Mahboob

Vijayaraghavan

et al. 2020

Saudi Journal of Biological Sciences

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Extensive application of pesticide in agricultural field affects the enzymatic activity of non-target animals, including fishes. In this study, the impact of sublethal concentration of fenvalerate on marker enzymes of freshwater Zebra fish was evaluated. Pesticide-induced stress can specifically affect non target fishes, through elevated level of reactive oxygen species which is responsible for biochemical, cell metabolism and physiological activities. The oxidative stress mediated by fenvalerate at sub lethal concentrations after 28 days of exposure of Zebra fish. Following 28 days of exposure of pesticide, catalase, superoxide dismutase, aspartate amino transferases, alanine amino transferase, alkaline phosphatase and acid phosphatase were assessed. Results revealed reduction of superoxide dismutase activity after 28 days of exposure in sub lethal concentration of fenvalerate in liver and gills. In liver, catalase activity was found to be less in fenvalerate exposed fish than control fish. In liver, increase of 75.75% aspartate amino transferase and 38% increase in alanine amino transferase in gills. SGPT activity was relatively higher than SGOT suggests more contribution of phyruvalate than oxaloacetate formation. Fenvalerate induced changes in acid phosphatase and alkaline phosphatase activity in the liver and gills of Zebra fish after four weeks of exposure. Fenvalerate induced expression of various stress proteins in gill, liver, followed by muscle. Some proteins lost its intensity due to fenvalerate toxicity. Result revealed that enzyme assays and SDS-PAGE analysis for protein subunits determination is relevant tool to monitor stress in freshwater ecosystem. The findings suggest that in monitoring fenvalerate toxicity programme, enzyme activities can be potent diagnostic tool for fenvalerate induced toxicity.

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“…From the spirulina transcriptome, a full‐length Ap Prx cDNA sequence was identified which was obtained by Illumina NextSeq500 technology, as provided in our earlier study (Arockiaraj, Bhatt, Kumaresan, et al, 2015; Kumaresan, Sannasimuthu, Arasu, Al‐Dhabi, & Arockiaraj, 2018). The physicochemical properties of Ap Prx were analyzed using various web programs, as described in our earlier report (Sannasimuthu et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

Peroxiredoxin of Arthrospira platensis derived short molecule YT12 influences antioxidant and anticancer activity

Sannasimuthu

Ramani

Pasupuleti

et al. 2020

Cell Biology International

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This study demonstrates both the antioxidant and anticancer potential of the novel short molecule YT12 derived from peroxiredoxin (Prx) of spirulina, Arthrospira platensis (Ap). ApPrx showed significant reduction in reactive oxygen species (ROS) against hydrogen peroxide (H 2 O 2) stress. The complementary DNA sequence of ApPrx contained 706 nucleotides and its coding region possessed 546 nucleotides between position 115 and 660. Real-time quantitative reverse transcription polymerase chain reaction analysis confirmed the messenger RNA expression of ApPrx due to H 2 O 2 exposure in spirulina cells at regular intervals, in which the highest expression was noticed on Day 20. Cytotoxicity assay was performed using human peripheral blood mononuclear cells, and revealed that at 10 μM, the YT12 did not exhibit any notable toxicity. Furthermore, ROS scavenging activity of YT12 was performed using DCF-DA assay, in which YT12 scavenged a significant amount of ROS at 25 μM in H 2 O 2-treated blood leukocytes. The intracellular ROS in human colon adenocarcinoma cells (HT-29) was regulated by oxidative stress, where the YT12 scavenges ROS in HT-29 cells at 12.5 μM. Findings show that YT12 peptide has anticancer activity, when treated against HT-29 cells. Through the MTT assay, YT12 showed vital cytotoxicity against HT-29 cells. These finding suggested that C Cell ell B Biology iology I International nternational Abbreviations: Ap, Arthrospira platensis; Cp, peroxidatic cys; DCF-DA, 2′,7′-dichlorofluorescein diacetate; H 2 O 2 , hydrogen peroxide; HT-29, human colon adenocarcinoma cell; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; PBMC, human peripheral blood mononuclear cell; Prx, peroxiredoxin; TEAC, Trolox equivalent antioxidant capacity.

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Fish chemokines 14, 20 and 25: A comparative statement on computational analysis and mRNA regulation upon pathogenic infection

Cited by 18 publications

References 44 publications

An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

Sub-lethal effect of synthetic pyrethroid pesticide on metabolic enzymes and protein profile of non-target Zebra fish, Danio rerio

Peroxiredoxin of Arthrospira platensis derived short molecule YT12 influences antioxidant and anticancer activity

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