FISH Chromosome Analysis of Plutonium Workers from the Sellafield Nuclear Facility

Tawn, E. Janet; Whitehouse, Caroline; Riddell, A.E.

doi:10.1667/rr3530.1

Cited by 17 publications

(11 citation statements)

References 32 publications

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“…Support for this comes from the in vitro work on long-term cultures reported here. Nevertheless, stable cells containing multiple aberrations are induced by a-particle radiation, albeit in small numbers, and have been observed in studies of workers exposed to internally deposited plutonium (Tawn et al 1985;Whitehouse et al 1998;Anderson et al 2005;Hande et al 2005;Livingston et al 2006;Tawn et al 2006;Sotnik et al 2014). In the current study, such cells were found in three of the nine men (Table 3).…”

Section: Discussionmentioning

confidence: 99%

“…A number of studies have characterized the chromosome aberrations observed in peripheral blood lymphocytes from nuclear workers exposed to a-particle-emitting internally deposited plutonium and attempted to relate these to the cumulative internal dose to the bone marrow (Brandom et al 1979;Tawn et al 1985;Brandom et al 1990;Whitehouse et al 1998;Hande et al 2003;Mitchell et al 2004;Anderson et al 2005;Hande et al 2005;Tawn and Whitehouse 2005;Livingston et al 2006;Tawn et al 2006;Sotnik et al 2014). The majority have identified translocations as the predominant aberration but, although their frequency is greater than that expected from any associated external low LET c-ray exposure, little has been achieved in terms of using translocation frequency to quantify the response following in vivo a-particle exposure.…”

Section: Introductionmentioning

confidence: 99%

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Chromosome aberrations in workers with exposure to α-particle radiation from internal deposits of plutonium: expectations from in vitro studies and comparisons with workers with predominantly external γ-radiation exposure

Sotnik

et al. 2015

Radiat Environ Biophys

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mFISH analysis of chromosome aberration profiles of 47 and 144 h lymphocyte cultures following exposure to 193 mGy α-particle radiation confirmed that the frequency of stable aberrant cells and stable cells carrying translocations remains constant through repeated cell divisions. Age-specific rates and in vitro dose-response curves were used to derive expected translocation yields in nine workers from the Mayak nuclear facility in Russia. Five had external exposure to γ-radiation, two of whom also had exposure to neutrons, and four had external exposure to γ-radiation and internal exposure to α-particle radiation from incorporated plutonium. Doubts over the appropriateness of the dose response used to estimate translocations from the neutron component made interpretation difficult in two of the workers with external exposure, but the other three had translocation yields broadly in line with expectations. Three of the four plutonium workers had translocation yields in line with expectations, thus supporting the application of the recently derived in vitro α-particle dose response for translocations in stable cells. Overall this report demonstrates that with adequate reference in vitro dose-response curves, translocation yield has the potential to be a useful tool in the validation of red bone marrow doses resulting from mixed exposure to external and internal radiation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chromosome aberrations in workers with exposure to α-particle radiation from internal deposits of plutonium: expectations from in vitro studies and comparisons with workers with predominantly external γ-radiation exposure

Sotnik

et al. 2015

Radiat Environ Biophys

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“…Also added to the exposed group were results from 31 men with between 1,000-2,000 cells analyzed. While these 31 men were originally classified as plutonium workers (34), a reassessment of their doses showed that they had internal red bone marrow a-particle doses of ,10 mGy. This brought the final exposed group from the earlier sampling to 228 individuals, and thus the total number now included in the results from the earlier sampling was 321.…”

Section: Chromosome Aberration Analysismentioning

confidence: 99%

Chromosome Aberrations Determined by FISH in Radiation Workers from the Sellafield Nuclear Facility

et al. 2015

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Workers from the Sellafield nuclear facility (Cumbria, UK) with occupational exposures to external sources of ionizing radiation were examined for translocation frequencies in peripheral blood lymphocytes using fluorescence in situ hybridization (FISH). This is an extension of an earlier study of retired workers, and includes analyses of additional samples from the earlier collection, bringing the total to 321. Another 164 samples from both current and retired employees, including 26 repeat samples, were obtained from a new collection, thus giving a combined dataset of 459 workers. This all-male population of workers was divided into 6 dose groups comprising 97 with recorded external occupational doses <50 mGy, 118 with 50-249 mGy, 129 with 250-499 mGy, 89 with 500-749 mGy, 17 with 750-999 mGy and 9 with >1,000 mGy. Univariate analysis showed a significant association between external dose and translocation frequency (P < 0.001) with the estimate of slope ± standard error being 1.174 ± 0.164 × 10(-2) translocations per Gy. Multivariate analysis revealed that age increased the rate of translocations by 0.0229 ± 0.0052 × 10(-2) per year (P < 0.001). However, the impact of age adjustment on the radiation dose response for translocation frequencies was minor with the new estimate of slope ± standard error being 1.163 ± 0.162 × 10(-2) translocations per Gy. With the dose response for the induction of translocations by chronic in vivo low-LET radiation now well characterized, cytogenetic analysis can play an integral role in retrospective dose reconstruction of chronic exposure in epidemiological studies of exposed populations.

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“…1). Même si quelques études ont montré une décroissance du taux de translocations avec le temps (Bauchinger et al, 2001 ;Lindholm et Edwards, 2004 ;Sorokine-Durm et al, 2000a ;Tucker, 2008), la stabilité des translocations est incontestablement meilleure que celle des dicentriques Grégoire et al, 2006 ;Lindholm et Edwards, 2004 ;Pala et al, 2001 ;Tawn et al, 2006 ;Tucker, 2008). L'étude de patients traités par radiothérapie durant des dizaines d'années pour spondylarthrite ankylosante, suggère que les translocations semblent persister plusieurs années après l'exposition (Buckton et al, 1962).…”

Section: Introductionunclassified

Impact des facteurs individuels et environnementaux sur le taux d’aberrations chromosomiques de type translocations Partie 1 : âge, sexe, tabac, alcool

et al. 2010

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The assessment of exposure to ionizing radiation, carried out long time after exposure, is currently performed by scoring of translocations, a specific type of chromosomal aberrations. The translocations rate observed in peripheral blood lymphocytes of exposed subjects is compared to that observed in a control population. However, the translocation specificity towards radiation exposure is not clearly identified. To avoid any hasty conclusion, it is necessary to identify all the factors likely to induce translocation. To our knowledge, no study has thus far examined the effects of all these different factors on translocation rates. A review of the literature thus allowed us to assess the impact of host factors and lifestyle on the production of translocations. This study confirms that age appears to be the factor 1 IRSN, Laboratoire de Dosimétrie Biologique, BP 17, 92262 Fontenay-aux-Roses Cedex, France.Article publié par EDP Sciences E. GRÉGOIRE et al. 154 RADIOPROTECTION -VOL. 45 -N° 2 (2010) having the greatest impact on the rate of translocations, especially over 60 years. To date, the factor "age" is already considered in estimating the impact of radiation on the rate of translocation for all age groups. However, the study also shows that this rate varies significantly when the patient is exposed simultaneously and significantly towards many lifestyle agents. A precise threshold translocation rate should thus be established as a function of known behavioral exposures, below which it is impossible to conclude that radiological exposure has occurred. The effects of chemicals on the translocation rate after occupational exposure will be the subject of a second part.

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FISH Chromosome Analysis of Plutonium Workers from the Sellafield Nuclear Facility

Cited by 17 publications

References 32 publications

Chromosome aberrations in workers with exposure to α-particle radiation from internal deposits of plutonium: expectations from in vitro studies and comparisons with workers with predominantly external γ-radiation exposure

Chromosome aberrations in workers with exposure to α-particle radiation from internal deposits of plutonium: expectations from in vitro studies and comparisons with workers with predominantly external γ-radiation exposure

Chromosome Aberrations Determined by FISH in Radiation Workers from the Sellafield Nuclear Facility

Impact des facteurs individuels et environnementaux sur le taux d’aberrations chromosomiques de type translocations Partie 1 : âge, sexe, tabac, alcool

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