We present new stable isotope (δ 18 O calcite and δ 13 C calcite ) and diatom data from a 67-m sediment core (BAN II) from Lake Banyoles, northeastern Spain. We reassessed the chronology of the sequence by correlating stable isotope data with a shorter U-series-dated record from the lake, confirming a sedimentological offset between the two cores and demonstrating that BAN II spans Marine Isotope Stages (MIS) 3 to 1. Through comparison with previous records, the multi-proxy data are used to improve understanding of palaeolimnological dynamics and, by inference, western Mediterranean climate and environmental change during the past ca. 50,000 years. Three main zones, defined by isotope and diatom data, correspond to the MIS. The basal zone (MIS 3) is characterised by fluctuating δ 18 O calcite and benthic diatom abundance, indicating a high degree of environmental and climate variability, concomitant with large lake-level changes. During the full glacial (MIS 2), relatively constant δ 18 O calcite and a poorly preserved planktonicdominated diatom assemblage suggest stability, and intermittently, unusually high lake level.In MIS 1, δ 18 O calcite and δ 13 C calcite initially transition to lower values, recording a pattern of Late Glacial to Holocene change that is similar to other Mediterranean records. This study suggests that Lake Banyoles responds limnologically to changes in the North Atlantic oceanatmosphere system and provides an important dataset from the Iberian Peninsula, a region in need of longer-term records that can be used to correlate between marine and terrestrial archives, and between the western and eastern Mediterranean.4