Fish fins and scales as non-lethally sampled tissues for stable isotope analysis in five fish species of north – eastern Spain

Cano-Rocabayera, Oriol; Maceda‐Veiga, Alberto; Sostoa, Adolfo de

doi:10.1007/s10641-014-0328-6

Cited by 32 publications

(26 citation statements)

References 24 publications

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“…In our study, C. sordidus had a positive slope in the muscle – δ 15 N relationship, and a negative slope for the blood – δ 15 N relationship, resulting in this being the only species that displayed an effect of TL on Δ δ N. This species also showed a significant difference in feeding between smaller IP and larger TP individuals (Plass‐Johnson et al., ). These results confirm the suggestion that conversion factors are species‐specific (Matich et al., ; Willis et al., ; Cano‐Rocabayera et al., ), even with closely related species (in our case two Chlorurus species) having different conversion factors.…”

Section: Discussionsupporting

confidence: 92%

“…Much work has gone into understanding alternative methods of isotopic sampling in teleost fishes (e.g. Matich et al., ; Willis et al., ; Cano‐Rocabayera et al., ), and the implication of our findings is that sampling of parrotfish blood for isotopic analysis could be an alternative to the highly destructive dorsal or caudal muscle sampling. Although extraction of blood from the caudal vein does not eliminate the chance of mortality, it clearly is potentially less destructive than sampling muscle.…”

Section: Discussionmentioning

confidence: 77%

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The effects of tissue type and body size on δ ¹³ C and δ ¹⁵ N values in parrotfish (Labridae) from Zanzibar, Tanzania

2015

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Differences between the stable isotopic ratios (d 13 C and d 15 N) of two tissues (blood and muscle) from four species of East African coral reef parrotfishes (family: Labridae, tribe: Scarini) were analysed across a broad spectrum of body sizes. Comparison of isotopic ratios between the tissues allowed the assessment of using blood as an alternative tissue to muscle. In 2010-2011, constant differences between tissues (d blood minus d muscle ) were found across a broad range of sampled fish lengths. Linear relationships between the tissues, specific for an isotope, indicate that constants could be generated for converting blood isotope into muscle isotope values. Only one species, Chlorurus sordidus, displayed an inconsistent difference between tissues in d 15 N, indicating that this ratio was dependent on fish length. The d 13 C of both tissues was positively related linearly to fish length for three species, while d 15 N showed no relationship with body length. The results are interpreted as indicating dietary consistency over days to weeks, the time of tissue turnover for blood and muscle, respectively. Lastly, differences among the species, even closely related species, show that the generation of tissue conversion constants is species-specific.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 77%

The effects of tissue type and body size on δ ¹³ C and δ ¹⁵ N values in parrotfish (Labridae) from Zanzibar, Tanzania

2015

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“…The rationale is that a consumer incorporates the isotopic composition of its prey in a predictive way in relation to an ecosystem baseline (Jardine et al, 2003). However, growing evidence indicates that isotopic assimilation (discrimination factors) can vary between species and their tissues (Bond and Diamond, 2011;Cano-Rocabayera et al, 2015). Since such knowledge is still very limited for many species in European Mediterranean rivers, these tracers should only be used after an accurate calculation of discrimination factors and a solid ecological knowledge of the target species (Bond and Diamond, 2011).…”

Section: Indicators Of Energy Metabolism and Trophic Tracersmentioning

confidence: 99%

Ecological relevance of biomarkers in monitoring studies of macro-invertebrates and fish in Mediterranean rivers

Colin

Porte

Fernandes

et al. 2016

Science of The Total Environment

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143

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“…Lethal sampling is, however, not desirable, particularly when studying highly threatened fish species or where anaesthesia may reduce the ability of the animal to recover. Several recent studies have used non‐lethal sampling approaches, including the sampling of mucus, scales, blood, and fin, as alternatives to white muscle. For most fish species, fin tissue has been found to be the most isotopically similar to muscle tissue, making fins viable alternatives to muscle tissue .…”

Section: Introductionmentioning

confidence: 99%

The influence of diet composition and tissue type on the stable isotope incorporation patterns of a small‐bodied southern African minnow Enteromius anoplus (Cypriniformes, Cyprinidae)

Kambikambi

Chakona

2019

Rapid Comm Mass Spectrometry

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Rationale In trophic ecology, the use of stable isotope data relies on the general understanding of isotope turnover rates and diet‐to‐tissue discrimination factors (DTDFs). Recent studies on the application of stable isotope data have shown that isotope turnover rates and DTDFs can be influenced by many factors, including diet composition and tissue type. This study investigated the influence of diet composition and tissue type on stable isotope incorporation patterns in a small‐bodied African minnow, the chubbyhead barb Enteromius anoplus. Methods The isotopic incorporation patterns of carbon (δ13C values) and nitrogen (δ15N values) into white muscle and caudal fin tissues of the chubbyhead barb were examined using two isotopically different diets. Controlled‐diet stable isotope feeding trials using a fishmeal‐based diet (diet 1) and a soya‐based diet (diet 2) were conducted over a 180‐day period for the chubbyhead barb. Results The two diets had contrasting isotopic incorporation patterns: diet 1 was associated with progressively high δ13C and δ15N values, whereas diet 2 was associated with progressively low δ13C and δ15N values over time for both muscle and fin tissues. The δ13C turnover rates were similar for both tissues (56 and 61 days), whereas the δ15N turnover rates differed between fin and muscle tissue in both diets (diet 1 = 4 and 130 days, and diet 2 = 72 and 300 days, respectively). The DTDFs were similar for both tissues in diet 1 (Δ13C: −3.96 to −2.62‰, Δ15N: 1.98 to 2.61‰) and diet 2 (Δ13C: 4.05 to 5.24‰, Δ15N: 8.45 to 9.69‰). Conclusions These results suggest that fin tissue can potentially be used as an alternative for muscle tissue in food web studies with a reasonable level of error. The isotopic turnover rate and DTDFs estimates for E. anoplus, however, require consideration of diet composition because different diets may differ in their isotopic incorporation patterns.

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Fish fins and scales as non-lethally sampled tissues for stable isotope analysis in five fish species of north – eastern Spain

Cited by 32 publications

References 24 publications

The effects of tissue type and body size on δ ¹³ C and δ ¹⁵ N values in parrotfish (Labridae) from Zanzibar, Tanzania

The effects of tissue type and body size on δ ¹³ C and δ ¹⁵ N values in parrotfish (Labridae) from Zanzibar, Tanzania

Ecological relevance of biomarkers in monitoring studies of macro-invertebrates and fish in Mediterranean rivers

The influence of diet composition and tissue type on the stable isotope incorporation patterns of a small‐bodied southern African minnow Enteromius anoplus (Cypriniformes, Cyprinidae)

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Fish fins and scales as non-lethally sampled tissues for stable isotope analysis in five fish species of north – eastern Spain

Cited by 32 publications

References 24 publications

The effects of tissue type and body size on δ 13 C and δ 15 N values in parrotfish (Labridae) from Zanzibar, Tanzania

The effects of tissue type and body size on δ 13 C and δ 15 N values in parrotfish (Labridae) from Zanzibar, Tanzania

Ecological relevance of biomarkers in monitoring studies of macro-invertebrates and fish in Mediterranean rivers

The influence of diet composition and tissue type on the stable isotope incorporation patterns of a small‐bodied southern African minnow Enteromius anoplus (Cypriniformes, Cyprinidae)

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Product

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The effects of tissue type and body size on δ ¹³ C and δ ¹⁵ N values in parrotfish (Labridae) from Zanzibar, Tanzania

The effects of tissue type and body size on δ ¹³ C and δ ¹⁵ N values in parrotfish (Labridae) from Zanzibar, Tanzania