FISH—in Birds

Kretschmer, Rafael; Santos, Michelly da Silva dos; Furo, Ivanete de Oliveira; Oliveira, Edivaldo Herculano Corrêa de; Cioffi, Marcelo de Bello

doi:10.1201/9781003223658-23

Cited by 2 publications

(2 citation statements)

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“…The microsatellite sequences (GAA) n , (GAC) n , (CGG) n , (CAC) n , (CAG) n , (CAT) n , (GAG) n , (TAA) n , (TAC) n , (CAA) n , (GA) n , (CA) n , (GC) n , (TA) n , (C) n , and (A) n were labeled directly with Cy3 at the 5' end during synthesis (VBC Biotech, Vienna, Austria) and also used in the fluorescence in situ hybridization (FISH) experiments. We performed the FISH experiments following the protocol described by Kretschmer et al [45]. Briefly, after one hour of aging at 60 • C, chromosomal preparations were treated for one hour and thirty minutes with an RNAse solution (1.5 µL RNase A (10 mg/mL) in 1.5 mL 2× SSC).…”

Section: Fluorescence In Situ Hybridizations (Fish)mentioning

confidence: 99%

Satellitome Analysis in the Southern Lapwing (Vanellus chilensis) Genome: Implications for SatDNA Evolution in Charadriiform Birds

Kretschmer,

Toma,

Deon

et al. 2024

Genes

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Vanellus (Charadriidae; Charadriiformes) comprises around 20 species commonly referred to as lapwings. In this study, by integrating cytogenetic and genomic approaches, we assessed the satellite DNA (satDNA) composition of one typical species, Vanellus chilensis, with a highly conserved karyotype. We additionally underlined its role in the evolution, structure, and differentiation process of the present ZW sex chromosome system. Seven distinct satellite DNA families were identified within its genome, accumulating on the centromeres, microchromosomes, and the W chromosome. However, these identified satellite DNA families were not found in two other Charadriiformes members, namely Jacana jacana and Calidris canutus. The hybridization of microsatellite sequences revealed the presence of a few repetitive sequences in V. chilensis, with only two out of sixteen displaying positive hybridization signals. Overall, our results contribute to understanding the genomic organization and atDNA evolution in Charadriiform birds.

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Section: Fluorescence In Situ Hybridizations (Fish)mentioning

confidence: 99%

Satellitome Analysis in the Southern Lapwing (Vanellus chilensis) Genome: Implications for SatDNA Evolution in Charadriiform Birds

Kretschmer,

Toma,

Deon

et al. 2024

Genes

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“…The probes were labeled with biotin (Roche Diagnostics, Mannheim, Germany) or digoxigenin (Roche Diagnostics, Mannheim, Germany) by degenerate oligonucleotideprimed polymerase chain reaction (DOP-PCR) and detected with streptavidin-CY3 (Invitrogen, Waltham, EUA) or sheep anti-digoxigenin FITC (Invitrogen, Waltham, EUA), respectively. Protocols for hybridization, stringency washes and detection were as described previously by Kretschmer et al [23]. Chromosomes were counterstained with an antifade solution containing 4 0 ,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, St. Louis, MO, USA).…”

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

Comparative chromosome painting in three Pelecaniformes species (Aves): Exploring the role of macro and microchromosome fusions in karyotypic evolution

Seligmann,

Furo,

dos Santos

et al. 2023

PLoS ONE

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Pelecaniformes is an order of waterbirds that exhibit diverse and distinct morphologies. Ibis, heron, pelican, hammerkop, and shoebill are included within the order. Despite their fascinating features, the phylogenetic relationships among the families within Pelecaniformes remain uncertain and pose challenges due to their complex evolutionary history. Their karyotypic evolution is another little-known aspect. Therefore, to shed light on the chromosomal rearrangements that have occurred during the evolution of Pelecaniformes, we have used whole macrochromosome probes from Gallus gallus (GGA) to show homologies on three species with different diploid numbers, namely Cochlearius cochlearius (2n = 74), Eudocimus ruber (2n = 66), and Syrigma sibilatrix (2n = 62). A fusion between GGA6 and GGA7 was found in C. cochlearius and S. sibilatrix. In S. sibilatrix the GGA8, GGA9 and GGA10 hybridized to the long arms of biarmed macrochromosomes, indicating fusions with microchromosomes. In E. ruber the GGA7 and GGA8 hybridized to the same chromosome pair. After comparing our painting results with previously published data, we show that distinct chromosomal rearrangements have occurred in different Pelecaniformes lineages. Our study provides new insight into the evolutionary history of Pelecaniformes and the chromosomal changes involving their macrochromosomes and microchromosomes that have taken place in different species within this order.

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FISH—in Birds

Cited by 2 publications

References 0 publications

Satellitome Analysis in the Southern Lapwing (Vanellus chilensis) Genome: Implications for SatDNA Evolution in Charadriiform Birds

Satellitome Analysis in the Southern Lapwing (Vanellus chilensis) Genome: Implications for SatDNA Evolution in Charadriiform Birds

Comparative chromosome painting in three Pelecaniformes species (Aves): Exploring the role of macro and microchromosome fusions in karyotypic evolution

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