Fish mycobacteriosis: morphopathological and immunocytochemical aspects

Gómez, S.; Bernabé, A.; Gómez, M.A.; Navarro, J. A.; Sánchez, J.

doi:10.1111/j.1365-2761.1993.tb00857.x

Cited by 23 publications

(21 citation statements)

References 15 publications

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“…There are 3 species of Mycobacterium recognised as potential agents of fish disease, namely M. chelonae, M. fortuitum and M. marinum (Inglis et al 1993, Belas et al 1995. Typical gross pathology including emaciation, exophthalmos, keratitis and skin ulcers; and abnormal swimming behaviour has been observed (Beckwith & Malsberger 1980, van Duijn 1981, Gomez et al 1993. Involvement of the…”

Section: Introductionmentioning

confidence: 99%

Pathology attributed to Mycobacterium chelonae infection among farmed and laboratory-infected Atlantic salmon Salmo salar

Bruno

Griffiths²,

Mitchell³

et al. 1998

Dis. Aquat. Org.

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This study was promoted following concern over increasing mortality on 2 farms rearing Atlantic salmon Salmo salar in the Shetland Isles, Scotland. A Mycobacterium sp. was isolated from monbund, market-s~zed Atlantic salmon. Biochemical tests, 1ipi.d analys~s and PCR (polynlerase chain reaction) techniques confirmed the bacterium to be Mycobacterium chelonae. Multiple greyish-white miliary granuloma-like nodules were observed in several tissues. Dense hard-packed nodules contained abundant acid-fast bacteria. Atlantic salmon injected with M. chelonae remained sub-clinically infected, demonstrating the chronic nature of this disease. The source of the pathogen was not identified.

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Section: Introductionmentioning

confidence: 99%

Pathology attributed to Mycobacterium chelonae infection among farmed and laboratory-infected Atlantic salmon Salmo salar

Bruno

Griffiths²,

Mitchell³

et al. 1998

Dis. Aquat. Org.

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“…The possibility exists that splenic granulomas may be absent from early infections due to an insufficient hypersensitive response of the host. On the other hand, reliance on granuloma detection as evidence for mycobacteriosis can also lead to false positive results since granulomas can be caused by microorganisms other than mycobacteria (Gomez et al 1993, Falkinham 2002. In this instance, however, there are numerous reports of typical granulomatous development due to mycobacteria in wild striped bass from the Chesapeake Bay (Heckert et al 2001, Rhodes et al 2001, Gauthier et al 2003.…”

Section: Resultsmentioning

confidence: 99%

“…(Shotts & Teska 1989, Rhodes et al 2004. Harsh decontamination techniques, originally designed for mammalian mycobacteriosis, are generally employed in order to reduce the nonmycobacterial microbiota which can overgrow the mycobacteria (Majeed et al 1981, Gomez et al 1993, Chinabut 1999. However, decontamination methods can adversely affect mycobacterial viability and lead to an underestimation of mycobacterial infection intensity (Dailloux et al 1999, Rhodes et al 2004.…”

Section: Reports Of Mycobacteriosis In Striped Bassmentioning

confidence: 99%

Comparative analysis of mycobacterial infections in wild striped bass Morone saxatilis from Chesapeake Bay

Kaattari¹,

Rhodes²,

Kator³

et al. 2005

Dis. Aquat. Org.

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During an ongoing epizootic of mycobacteriosis, wild striped bass Morone saxatilis from Chesapeake Bay were analyzed using 3 methods for detection of either mycobacterial infection or associated granulomatous pathology. The specific detection techniques, which utilized aseptically collected splenic tissue, were histology, quantitative culture and nested PCR. Based on analysis of 118 samples, detection of infection differed significantly between the 3 methods (chi-square, p = 0.0007). Quantitative culture and nested PCR detected similar, higher rates of infection (69 and 75%, respectively) than the histological method (52%). Although primary PCR assays for a 924 to 940 bp segment of the mycobacterial 16S rRNA gene were positive for genomic DNA from mycobacterial cultures, a secondary, nested PCR reaction for an internal 300 bp gene segment was required in order to detect mycobacteria within splenic tissue. A similar rate of mycobacterial infection was present in fish collected from all sites tested. Although all detection methods found that striped bass age 4.0 to 4.9 yr had the highest positive incidence, nested PCR detected a higher frequency of mycobacterial infection in fish ≥ 6.0 yr of age than the other 2 methods. Quantitative bacteriology was a more sensitive detection technique when the fish tissue contained ≤10 3 mycobacteria g -1 .

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“…These fish survive quite well due to the labyrinth above their gills, an organ which acts as an internal air reservoir and for the adjustment of their metabolism. Nevertheless, they still suffer from microbial and viral attacks [43][44][45] . The Department of Fisheries of Thailand is developing a more rapid and specific diagnosis for pathogens in addition to the external examinations for parasites and bacteria.…”

Section: Diseases and Their Preventionmentioning

confidence: 99%

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Monvises¹,

Nuangsaeng²,

Sriwattanarothai³

et al. 2009

ScienceAsia

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ABSTRACT:The Siamese fighting fish, of which Betta splendens is representative, is gaining popularity as judged by increasing export values and demands for novel types worldwide, especially the ornamental ones. However, relatively little is known about the bettas scientifically. In this review we cover what is known about the desired morphology and pigments, genetics, aquaculture, diseases and feeds involved in breeding bettas. We also propose breeding the bettas for domestic enjoyment and export by exploiting current knowledge of gene technology and molecular developmental biology in addition to the use hitherto of only classical genetics. Other aspects of fish culture such as feeds, disease prevention and water quality should also be scientifically studied and improved upon. Because of dwindling and worsening habitats in Thailand, more studies on biodiversity and work towards species conservation of the wild types are needed.

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Fish mycobacteriosis: morphopathological and immunocytochemical aspects

Cited by 23 publications

References 15 publications

Pathology attributed to Mycobacterium chelonae infection among farmed and laboratory-infected Atlantic salmon Salmo salar

Pathology attributed to Mycobacterium chelonae infection among farmed and laboratory-infected Atlantic salmon Salmo salar

Comparative analysis of mycobacterial infections in wild striped bass Morone saxatilis from Chesapeake Bay

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