Fish oil ingestion reduces the number of aberrant crypt foci and adenoma in 1,2-dimethylhydrazine-induced colon cancer in rats

Moreira, A.P.B.; Sabarense, C.M.; Dias, C.M.G.C.; Lunz, W.; Natali, A.J.; Glória, M.B.A.; Peluzio, M.C.G.

doi:10.1590/s0100-879x2009001200008

Cited by 18 publications

(12 citation statements)

References 16 publications

(23 reference statements)

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“…In addition, the results showed that at 4 or 17 weeks after cancer induction by DMH without fish oil supplements (DMH4 and DMH17) the DNA damage, alterations in the expressions of the tested genes and the histopathological lesions, still remained in the tissues. These results are in agreement with previous findings (Cheng et al, 2003, Anilakumar et al, 2004, Kamaleeswari et al, 2006, Jwanny et al, 2009Moreira et al, 2009 andHill et al, 2010).…”

Section: Discussionsupporting

confidence: 94%

Dietary fish oil modulates the effect of dimethylhydrazine- induced colon cancer in rats

Rasmy

Khalil²,

Moharib

et al. 2011

Grasas y Aceites

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In order to induce colon cancer, the rats were given a weekly subcutaneous injection of 1,2-Dimethylhydrazine (DMH) at a dose of 20 mg/kg b.w. for five weeks. Afterwards, some of the rats ingested fish oil for either 4 weeks (DMH-FO4 group), or 17 weeks (DMH-FO17 group). The remaining rats continued without any supplementation for the same 4 weeks (DMH4 group), or 17 weeks (DMH17 group). Another two groups of rats did not receive the DMH and were given fish oil (FO17 group) or a normal diet only and considered as the control group (CN group). At the end of the experiment, the rats were sacrificed; and were subsequently subjected to biochemical and molecular biological analyses as well as histopathological examinations. The results showed increased levels of lactate dehydrogenase (LDH), malondialdehyde (MDA) and alkaline phoshatase (ALP) activities in the DMH rats compared to the control. The liver and colonic changes that were induced by DMH were significantly improved through fish oil supplementation in the DMH-FO17 group. The molecular analysis revealed that DMH treatment induced the expression alterations of genes p53, p27 and p21 and increased DNA band patterns related to cancer, while both FO17 and DMH-FO17 groups showed much better results. A histopathological examination of the DMH17 group revealed colon adenocarcinoma and several lesions in rat liver tissues. An improvement in the histopathological picture was seen in the livers and colons of groups DMH-FO17. In conclusion, the present results demonstrated the anti-carciongenic effect of herring fish oil against DMH induced colon carcinogenesis in rats. The inhibitory effect of FO was due to the modulation of elevated biochemical parameters, DNA damage, gene expression and histopathological lesions caused by DMH. KEY-WORDS: Colorectal cancer -DMH -Fish oil -Gene expression -Rats. INTRODUCTIONAmong the factors that contribute to the appearance of cancer, diet plays a fundamental role. Fats are the main component related to the increase in the incidence of cancerous diseases, particularly breast, prostate and colorectal cancer. Colon cancer is one of the leading causes of death in both men and women in Western countries (Parker et al., 1995;and Jemal et al., 2003) and it became the number six leading cause of cancer deaths in Egypt (WHO report, 2006). Several RESUMEN Efecto del aceite de pescado de la dieta en cáncer de colon inducido por dimetilhidrazina en ratas.Este estudio fue realizado para examinar la eficacia de la suplementación de aceite de pescado en la carcinogéne-sis de colon en ratas wistar machos. Para la inducción del cáncer de colon, las ratas fueron tratadas semanalmente con una inyección subcutánea de 1,2-dimethyl hydrazine (DMH) a una dosis de 20 mg/kg de peso durante cinco semana. A continuación, algunas ratas tomaron aceite de pescado durante 4 semanas, o durante 17 semanas (grupo DMH-FO17). Las ratas restantes continuaron sin ningún tipo de suplementación durante las mismas 4 semanas (grupo DMH4), o 17 semanas (grupo DMH17). Otros dos...

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Section: Discussionsupporting

confidence: 94%

Dietary fish oil modulates the effect of dimethylhydrazine- induced colon cancer in rats

Rasmy

Khalil²,

Moharib

et al. 2011

Grasas y Aceites

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“…Although the application of probiotic with oil, specifically with Lini oleum virginale, to rats was not documented, the effect of fish oil ingestion on colonic carcinogenesis in rats was studied by Moreira et al (2009).…”

Section: Histopathological Changesmentioning

confidence: 99%

Effects of a probiotic in combination with prebiotics on intestinal lactobacilli and coliforms and activities of bacterial enzymes in 1,2-dimethylhydrazine exposed rats

Strojný¹,

Bomba²,

Hijová³

et al. 2011

Czech J. Anim. Sci.

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The intestinal microflora plays a crucial role in the health of humans and animals. All functions of the absorption of nutrients are connected with the valid digestive tract. Approximately 70% of the immune function is derived from the gut, being composed of the mucosal barrier, submucosal glands and the mucosa-associated lymphoid tissue (Bengmark, 2002 ABSTRACT: Effects of the probiotic (PRO) Lactobacillus plantarum and of the combination of PRO and the prebiotic (PRE) inulin enriched with oligofructose (2%), and PRO with Lini oleum virginale (O) on counts of lactobacilli and coliforms and enzymatic activities in faeces of rats were studied. The rats (n = 60) were divided into 5 groups of 12 subjects. The animals were fed on a high fat diet (10%) for 8 weeks of experiment. Colon cancer was induced by the application of 1,2-dimethylhydrazine (DMH) twice a week in a dose of 20 mg/kg s.c. in groups G2-G5. The rats in group 1 (control 1) received a diet without any supplements. The rats in group 2 (control 2) received 1,2 DMH without any supplements. The rats in group 3 received PRO, group 4 PRO and PRE, and group 5 received PRO and O. A significant decrease (P < 0.05) of coliforms was found out after the application of PRO, PRO-O, and PRO-PRE in comparison with control group G2. Significantly higher (P < 0.05) counts of lactobacilli were determined after the application of PRO-O and PRO-PRE. Significantly lower (P < 0.001) activities of β-galactosidase, β-glucuronidase and α-glucosidase were observed in PRO, PRO-PRE and PRO-O, while in the case of the enzyme β-glucosidase the activity was lower only after the addition of PRO-O. The protective effect of lactobacilli was observed in the order PRO-O, PRO-PRE, and PRO. It was shown that combinations of PRO-O and PRO-PRE had a synergistic effect which was higher than the effect of administering only PRO.

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“…The results of their experiment indicate that probiotic microorganisms and bioactive compounds could exert a preventive effect on colon carcinogenesis induced by dimethylhydrazine. Although the administration of probiotic with oil, specifically with Lini oleum virginale, to rats was not documented, the effect of fish oil ingestion on colonic carcinogenesis in rats was studied by Moreira et al (2009). The results obtained from our study indicate a positive effect of administering the combination of the strain L. plantarum as probiotic with oil.…”

Section: Discussionmentioning

confidence: 65%

The effect of new probiotic strain Lactobacillus plantarum on counts of coliforms, lactobacilli and bacterial enzyme activities in rats exposed to N,N-dimethylhydrazine (chemical carcinogen)

et al. 2012

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The aim of the present study was to evaluate the effect of the new probiotic strain Lactobacillus plantarum on chemically induced carcinogenesis in rats. Sprague dowley rats (n = 33) were divided into control and experimental groups and were fed a conventional laboratory diet. In the experimental group, rats were treated with the probiotic at the dose of 1 × 10 9 CFU (colonyforming units)/ml. Two weeks after the beginning of the trial, N,N-dimethylhydrazine (chemical carcinogen) injections were applied s.c. at the dose of 21 mg/kg b.w., 5 × weekly. At the end of the 8-month experimental period, faeces samples were taken from the rats and used for laboratory analysis. The counts of lactobacilli and coliforms and bacterial enzyme activity were determined. The probiotic strain L. plantarum as single species or in combination with oil (Lini oleum virginale) decreased the count of total coliforms and increased lactobacilli in faeces of rats. Application of probiotic microorganisms significantly (P < 0.05) decreased the activities of bacterial enzymes (β-galactosidase and β-glucuronidase) compared to the control group rats. The results of this study indicate that probiotic microorganisms could exert a preventive effect on colon carcinogenesis induced by N,N-dimethylhydrazine.

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Fish oil ingestion reduces the number of aberrant crypt foci and adenoma in 1,2-dimethylhydrazine-induced colon cancer in rats

Cited by 18 publications

References 16 publications

Dietary fish oil modulates the effect of dimethylhydrazine- induced colon cancer in rats

Dietary fish oil modulates the effect of dimethylhydrazine- induced colon cancer in rats

Effects of a probiotic in combination with prebiotics on intestinal lactobacilli and coliforms and activities of bacterial enzymes in 1,2-dimethylhydrazine exposed rats

The effect of new probiotic strain Lactobacillus plantarum on counts of coliforms, lactobacilli and bacterial enzyme activities in rats exposed to N,N-dimethylhydrazine (chemical carcinogen)

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