Fish species identification in canned pet food by BLAST and Forensically Informative Nucleotide Sequencing (FINS) analysis of short fragments of the mitochondrial 16s ribosomal RNA gene (16S rRNA)

Armani, Andrea; Tinacci, Lara; Xiong, Xiong; Castigliego, Lorenzo; Gianfaldoni, Daniela; Guidi, Alessandra

doi:10.1016/j.foodcont.2014.10.018

Cited by 45 publications

(42 citation statements)

References 35 publications

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“…Although DNA barcoding has been largely applied to different food sectors, studies on the traceability and mislabelling of pet foods are still scarce and generally performed on single species-based products using direct Sanger sequencing [23][24][25][26] or multiple species with qPCR assays 9 . To the best of our knowledge, the study by Xing et al represents the first (and, so far, only) attempt to investigate the species composition of pet food products by means of NGS-based DNA metabarcoding 27 , even though almost all of the products analysed (26 of 27) were for human consumption and the species within the products were limited to the Aves and Mammalia classes.…”

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confidence: 99%

NGS-based barcoding with mini-COI gene target is useful for pet food market surveys aimed at mislabelling detection

Palumbo

Scariolo

Vannozzi

et al. 2020

Sci Rep

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Pet food industry has grown considerably in the last few years and it is expected to continue with this rate. Despite the economic impact of this sector and the consumer concerns for the increasing number of food and feed adulteration cases, few studies have been published on mislabelling in pet foods. We therefore investigated the capability of a next generation sequencing-based mini-barcoding approach to identify animal species in pet food products. In a preliminary analysis, a 127 bp fragment of the COI gene was tested on both individual specimens and ad hoc mixed fresh samples used as testers, to evaluate its discrimination power and primers effectiveness. Eighteen pet food products of different price categories and forms available on the market (i.e. kibbles, bites, pâté and strips) were analysed through an NGS approach in biological replicates. At least one of the species listed in the ingredients was not detected in half of the products, while seven products showed supplementary species in addition to those stated on the label. Due to the accuracy, sensitivity and specificity demonstrated, this method can be proposed as food genetic traceability system to evaluate both the feed and food quality timely along the supply chain.

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confidence: 99%

NGS-based barcoding with mini-COI gene target is useful for pet food market surveys aimed at mislabelling detection

Palumbo

Scariolo

Vannozzi

et al. 2020

Sci Rep

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“…Other works regarding seafood mislabelling have been reported all over the world focused in different species: rockfish ( Logan et al, 2008 ), tuna from sushi in restaurants ( Lowenstein et al, 2009 ) and red snapper ( Marko et al, 2004 ) in the USA; cod ( Miller and Mariani, 2010 ), hake ( Machado-Schiaffino et al, 2008 ) and fish in pet food ( Armani et al, 2015b ) in Europe. All had rates of mislabelling higher than 20%.…”

Section: Resultsmentioning

confidence: 99%

Assessment of the labelling accuracy of spanish semipreserved anchovies products by FINS (forensically informative nucleotide sequencing)

Velasco

Aldrey

Pérez‐Martín

et al. 2016

Heliyon

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Anchovies have been traditionally captured and processed for human consumption for millennia. In the case of Spain, ripened and salted anchovies are a delicacy, which, in some cases, can reach high commercial values. Although there have been a number of studies presenting DNA methodologies for the identification of anchovies, this is one of the first studies investigating the level of mislabelling in this kind of products in Europe. Sixty-three commercial semipreserved anchovy products were collected in different types of food markets in four Spanish cities to check labelling accuracy. Species determination in these commercial products was performed by sequencing two different cyt-b mitochondrial DNA fragments. Results revealed mislabelling levels higher than 15%, what authors consider relatively high considering the importance of the product. The most frequent substitute species was the Argentine anchovy, Engraulis anchoita, which can be interpreted as an economic fraud.

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“…This gene has been shown to be a good marker also to differentiate fish species and it has been used in comparative intergeneric and interspecific studies in several fish families [34][35] . However, the cytb and COI genes, due to their comparable high interspecific and low intraspecific variation, are nowadays the most used genetic markers for fish species identification, as reported in a large number of studies applied to food inspection 8,[36][37][38] . A wide variety of universal primers is now available for the amplification of the three genes reported above.…”

Section: Universal Primers Analysismentioning

confidence: 99%

Seafood Identification in Multispecies Products: Assessment of 16SrRNA, cytb, and COI Universal Primers’ Efficiency as a Preliminary Analytical Step for Setting up Metabarcoding Next-Generation Sequencing Techniques

Giusti

Tinacci

Sotelo

et al. 2017

J. Agric. Food Chem.

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Few studies applying NGS have been conducted in the food inspection field, particularly on multispecies seafood products. A preliminary study screening the performance and the potential application in NGS analysis of 14 "universal primers" amplifying 16SrRNA, cytb, and COI genes in fish and cephalopods was performed. Species used in surimi preparation were chosen as target. An in silico analysis was conducted to test primers' coverage capacity by assessing mismatches (number and position) with the target sequences. The 9 pairs showing the best coverage capacity were tested in PCR on DNA samples of 53 collected species to assess their amplification performance (amplification rate and amplicon concentration). The results confirm that primers designed for the 16SrRNA gene amplification are the most suitable for NGS analysis also for identification of multispecies seafood products. In particular, the primer pair of Chapela et al. (2002) is the best candidate.

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Fish species identification in canned pet food by BLAST and Forensically Informative Nucleotide Sequencing (FINS) analysis of short fragments of the mitochondrial 16s ribosomal RNA gene (16S rRNA)

Cited by 45 publications

References 35 publications

NGS-based barcoding with mini-COI gene target is useful for pet food market surveys aimed at mislabelling detection

NGS-based barcoding with mini-COI gene target is useful for pet food market surveys aimed at mislabelling detection

Assessment of the labelling accuracy of spanish semipreserved anchovies products by FINS (forensically informative nucleotide sequencing)

Seafood Identification in Multispecies Products: Assessment of 16SrRNA, cytb, and COI Universal Primers’ Efficiency as a Preliminary Analytical Step for Setting up Metabarcoding Next-Generation Sequencing Techniques

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