Fish species identification in seafood products

Sotelo, Carmen G.; Piñeiro, Carmen; Gallardo, José Manuel; Pérez‐Martín, Ricardo I.

doi:10.1016/0924-2244(93)90043-a

Cited by 102 publications

(67 citation statements)

References 27 publications

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“…Several methods mainly based on protein separation by electrophoresis or high-performance liquid chromatography have been developed for species identification (Sotelo et al, 1993). In recent years, new DNA analyses have been finding their way into the identification of species, subspecies, populations, strains, hybrids and individuals.…”

mentioning

confidence: 99%

Discrimination of Shark species by simple PCR of 5S rDNA repeats

et al. 2008

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Sharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to discriminate elasmobranch species. The described 5S rDNA PCR approach generated species-specific genetic markers that should find broad application in fishery management and trade of sharks and their subproducts.

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mentioning

confidence: 99%

Discrimination of Shark species by simple PCR of 5S rDNA repeats

et al. 2008

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“…Various qualitative methods can be used for the detection of antigen-antibody reactions such as precipitation, immunodiffusion, immunoelectrophoresis or ELISA, which can also be used for quantitative assessment. Due to the fact that heat treatment may affect protein immunogenity, antibodies have been developed that can be used for fish species identification in cooked products (Sotelo et al, 1993). Using immunological methods, fish species identification in cooked and dried fish products including among others Alaska pollack (Theragra chalcogramma) was investigated (Ochiai and Watabe, 2003).…”

Section: Immunological Methodsmentioning

confidence: 99%

“…Their use is limited to fresh (unprocessed) fish. Chromatographic methods have been suggested for species quantification, particularly with regard to a high number of detector types developed for HPLC (Sotelo et al, 1993).…”

Section: Chromatographic Methodsmentioning

confidence: 99%

“…Species identification becomes much more difficult in fish that have undergone processing by the food industry and morphological criteria cannot be used (Sotelo et al, 1993;Aranishi, 2005;Dooley et al, 2005).…”

Section: Morphological Identificationmentioning

confidence: 99%

“…Heat resistant sarcoplasmic proteins (parvalbumins) and some myofibrillar proteins (Sotelo et al, 1993;Rehbein et al, 1999;Pineiro et al, 2001) are significant markers for electrophoretic discrimination between heat treated fish species. Several isoforms of parvalbumins -acidic, heat stable, calcium binding and low Mw protein -exist.…”

Section: Ief and Detection Of Heat Treated Fish Musculature Proteinsmentioning

confidence: 99%

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Methods of gadoid fish species identification in food and their economic impact in the Czech Republic: a review

Hubálková¹,

Králík²,

Tremlová³

et al. 2007

Vet. Med. - Czech

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ABSTRACT:The purpose of the present study was to give an overview of imported and traded gadoid fish species (Gadiformes) in the Czech Republic and to describe available methods for their authentication. Due to the increasing interest of customers in the purchase of buy fish meat and other seafood animals, it is necessary to have available analytical methods with discriminating power of respective fish species. With regard to different values and prices of various fish species, these may be adultered. Until recently, electrophoretic, chromatographic and immunological methods based on the analysis of proteins extracted from fish musculature seemed to be promising. Using these methods, various fish species can be identified in fresh, chilled and frozen products. However, they often fail in heat treated products. Molecular biology methods based on DNA analysis are more reliable and suitable for the analysis of fish products that have been heat treated during the production process.Keywords: cod fish; fish species identification; electrophoresis; PCR-RFLP; cytochrome b gene; food adulteration List of abbreviations AK = adenylate kinase; ARGK = arginine kinase; ATP = adenosine triphosphate; CE = capillary electrophoresis; CK = creatine kinase; CR = Czech Republic; DGGE = denaturating gradient gel electrophoresis; DHA = docosahexaen acid; DNA = deoxyribonucleotide acid; EPA = eicosapentaen acid; EU = European Union; FINS = forensically informative nucleotide sequencing; G 3-PD = glycerol 3-phosphate dehydrogenase; HPLC = high performance liquid chromatography; IEF = isoelectric focusing; LDH = lactate dehydrogenase; MDH = malate dehydrogenase; MS = mass spectrometry; mt cyt b = mitochondrial cytochrome b gene; Mw = molecular weight; NDKA = nucleoside diphosphate kinase A; NTSs = nontranscribed spacers; PCR = polymerase chain reaction; PCR-RAPD = polymerase chain reaction -random amplified polymorphic DNA; PCR-RFLP = polymerase chain reaction -restriction fragment length polymorphism; PCR-SSCP = polymerase chain reaction -single strand conformation polymorphism; pI = isoelectric point; RP-HPLC = reversed phased -high performance liquid chromatography; SDS-PAGE = sodium dodecyl sulphate -polyacrylamide gel electrophoresis; urea-IEF = urea isoelectric focusing; 2DE = two dimensional electrophoresis

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