The ichthyofauna of streams in the Neotropical region is not yet fully known. This study aims to investigate the ichthyofauna composition of six streams of the Ijuí River sub-basin, Rio Grande do Sul State, inserted in the Uruguay River basin, as to contribute to the knowledge of fishes species richness and distribution in the south of Brazil. Sampling was carried out between July 2015 and May 2016, bimonthly, using the technique of electric fishing to collect the fishes. Spatial variations (per sampled stream) in the ichthyofauna composition were tested with a permutational multivariate analysis of variance. In total, we collected 5,029 individuals from 55 species, 13 families and five orders. From these species, 17 are endemic to the Uruguay River basin. Five species alone represented approximately 70% of the ichthyofauna abundance sampled. Our hypothesis that the fish community composition is not homogeneous along the streams sampled was confirmed and we observed that species complexity increases from the upstream closest area to the downstream according to the river continuum concept.