Calibrated miniature pulse fission chambers (MPFCs) can be used for absolute measurement of the fission reaction rate in neutronic experiments. Absolute measurements require the effective number of fissionable atoms (ENFAs) in the coating of the chambers. An elimination method was proposed to calibrate the MPFCs embodying highly enriched and depleted uranium, using 14.75 MeV fast neutrons from the D–T reaction. The ENFAs of 235U in enriched uranium MPFC and 238U in depleted uranium MPFC are 8.39 × 1018 and 9.55 × 1018, with the uncertainties of 5.27% and 4.76%, respectively.