Fission yeast Ags1 confers the essential septum strength needed for safe gradual cell abscission

Cortés, Juan Carlos G.; Sato, Mamiko; Muñoz, Javier; Moreno, M. Belén; Clemente‐Ramos, José Ángel; Ramos, Mariona; Okada, Hitoshi; Osumi, Masako; Durán, Abraham Madroñal; Ribas, Juan Carlos

doi:10.1083/jcb.201202015

Cited by 86 publications

(201 citation statements)

References 56 publications

(97 reference statements)

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“…Consistent with this interpretation, Δrga7 cells and bgs4 + mutants cwg1-1 and cwg1-2 have similar septal defects and a similar tendency to lyse (Cortes et al, 2012;Martin-Garcia et al, 2014;Munoz et al, 2013).…”

Section: Role Of Rga7p In Post-golgi Transport Of Bgs4psupporting

confidence: 70%

“…The enzyme α-glucan synthetase 1, Ags1p, is the major contributor of α-D-glucans in the primary and the secondary septum in fission yeast (Cortes et al, 2012). Cells with mutations in mok1 + /ags1 + lyse during cell separation at the end of cytokinesis.…”

Section: Introductionmentioning

confidence: 99%

“…When cells have defects in secretion of ECM, contractile rings can retract before completion, resulting in multinucleate cells (Xu and Vogel, 2011). Yeast cells assemble an extracellular septum that primarily comprises β-Dglucan and α-D-glucan polymers (Cortes et al, 2012;Humbel et al, 2001) that is essential for the contractile ring constriction (Arasada and Pollard, 2014;Liu et al, 1999;Proctor et al, 2012;Sipiczki and Bozsik, 2000). A signaling pathway called the septation initiation network (SIN) couples contractile ring constriction with septum assembly (Simanis, 2015).…”

Section: Introductionmentioning

confidence: 99%

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A role for F-BAR protein Rga7p during cytokinesis in S. pombe

Arasada

Pollard

2015

Journal of Cell Science

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F-BAR proteins are known to participate in cytokinesis, but their mechanisms are not well understood. Here we investigated Rga7p an S. pombe F-BAR protein with a RhoGAP domain. Localization of Rga7p to the cytokinetic cleavage furrow depends on its F-BAR domain, actin filaments, formins Cdc12p and For3p, and the presence of a contractile ring. Rga7p is not required for the constriction of the contractile ring but does participate in the transport of a β-glucan synthetase Bgs4p from the late Golgi compartments to the plasma membrane adjacent to the contractile ring. Cells without Rga7p moved Bgs4p normally from the poles to the Golgi apparatus near the cell center, but Bgs4p then moved slowly from the late Golgi compartments to the cleavage site. The late arrival and lower than normal numbers of Bgs4p result in septal defects late in cytokinesis and lysis of separating cells similar to cells with the mutations in the cwg1+ gene encoding Bgs4p.

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Section: Role Of Rga7p In Post-golgi Transport Of Bgs4psupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A role for F-BAR protein Rga7p during cytokinesis in S. pombe

Arasada

Pollard

2015

Journal of Cell Science

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“…Death of rho1-596 is quite similar to that caused by decreasing the amount of Rho1 in a "switch-off" strain (Arellano et al 1997) or by reducing Rho1 activity through overexpression of a rho1T20N dominant negative allele (Arellano et al 1997). These strains do not die by rapidly losing the intracellular content, as occurs to mutants with defects in cell wall components (Cortes et al 2005(Cortes et al , 2012 or cell wall organization (De Medina-Redondo et al 2010). While overexpression of rho1 + completely rescued rho1-596 growth at 36°, overexpression of other Rho GTPases coding genes like rho2 + or rho3 + could not rescue its thermosensitive growth and was deleterious, suggesting that both GTPases play different cellular roles than Rho1.…”

Section: Discussionmentioning

confidence: 99%

Negative Functional Interaction Between Cell Integrity MAPK Pathway and Rho1 GTPase in Fission Yeast

et al. 2013

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Rho1 GTPase is the main activator of cell wall glucan biosynthesis and regulates actin cytoskeleton in fungi, including Schizosaccharomyces pombe. We have obtained a fission yeast thermosensitive mutant strain carrying the rho1-596 allele, which displays reduced Rho1 GTPase activity. This strain has severe cell wall defects and a thermosensitive growth, which is partially suppressed by osmotic stabilization. In a global screening for rho1-596 multicopy suppresors the pmp1 + gene was identified. Pmp1 is a dual specificity phosphatase that negatively regulates the Pmk1 mitogen-activated protein kinase (MAPK) cell integrity pathway. Accordingly, elimination of Pmk1 MAPK partially rescued rho1-596 thermosensitivity, corroborating the unexpected antagonistic functional relationship of these genes. We found that rho1-596 cells displayed increased basal activation of the cell integrity MAPK pathway and therefore were hypersensitive to MgCl 2 and FK506. Moreover, the absence of calcineurin was lethal for rho1-596. We found a higher level of calcineurin activity in rho1-596 than in wild-type cells, and overexpression of constitutively active calcineurin partially rescued rho1-596 thermosensitivity. All together our results suggest that loss of Rho1 function causes an increase in the cell integrity MAPK activity, which is detrimental to the cells and turns calcineurin activity essential. RHO GTPases are conserved proteins that regulate the actin cytoskeleton organization in all eukaryots (Heasman and Ridley 2008). Additionally, in yeast and other fungi, these GTPases provide the coordinated regulation of cell wall biosynthesis and actin organization, which is required to maintain cell integrity and polarized growth (Levin 2005;Park and Bi 2007;Perez and Cansado 2010). The Schizosaccharomyces pombe genome contains six genes coding Rho GTPases. Among them, rho1 + is essential (Arellano et al. 1996). Rho1 function is mediated by its interaction with at least three different targets: the b(1,3)-glucan synthase (Arellano et al. 1996), which is responsible for the synthesis of the major cell wall component, and the kinases Pck1 and Pck2 Sayers et al. 2000). Through both kinases, Rho1 also regulates the cell wall synthesis. Rho2 also interacts with Pck2 and, therefore, both GTPases regulate the a-D-glucan synthesis through Pck2 (Katayama et al. 1999;Calonge et al. 2000). Lack of Rho1 is lethal, and this phenotype is not suppressed by osmotic stabilization (Arellano et al. 1997), suggesting that defective biosynthesis of the cell wall is not the unique cause of death. On the contrary, Rho2-less cells are viable, although they become slightly rounded and more sensitive to treatment with glucanases (Hirata et al. 1998). Rho2 and Pck2 participate in the activation of the cell integrity mitogen-activated protein kinase (MAPK) signaling pathway (Ma et al. 2006). This signaling cascade responds to different extracellular stress stimuli such as hyper-or hypotonic conditions, oxidative stress, cell wall damaging compounds, and g...

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“…The septum staining by calcofluor was conducted based on the Dr. Paul Nurse’s Lab Fission Yeast Handbook (Cortés et al 2012). In brief, the yeast cells from late log phase culture (OD 600 = 2.0–3.0) was diluted to OD 600 = 0.05, 3 μg/ml honokiol was next added to the culture, and finally 10 7 cells was collected at different time points by centrifugation at 2500 rpm for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Antifungal compound honokiol triggers oxidative stress responsive signalling pathway and modulates central carbon metabolism

Wang

Yan

2016

Mycology

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The fast growing evidences have shown that the plant-derived compound honokiol is a promising candidate for treating multiple human diseases, such as inflammation and cancer. However, the mode-of-action (MoA) of honokiol remains largely unclear. Here, we studied the antifungal activity of honokiol in fission yeast model, with the goal of understanding the honokiol’s mechanism of action from the molecular level. We found that honokiol can inhibit the yeast growth at a dose-dependent way. Microarray analysis showed that honokiol has wide impacts on the fission yeast transcription levels (in total, 512 genes are up-regulated, and 42 genes are down-regulated). Gene set enrichment analysis indicated that over 45% up-regulated genes belong to the core environmental stress responses category. Moreover, network analysis suggested that there are extensive gene–gene interactions amongst the co-expression gene lists, which can assemble several biofunctionally important modules. It is noteworthy that several key components of central carbon metabolism, such as glucose transporters and metabolic enzymes of glycolysis, are involved in honokiol’s MoA. The complexity of the honokiol’s MoA displayed in previous studies and this work demonstrates that multiple omics approaches and bioinformatics tools should be applied together to achieve the complete scenario of honokiol’s antifungal function.

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Fission yeast Ags1 confers the essential septum strength needed for safe gradual cell abscission

Abstract: The α(1-3)glucan synthase Ags1 is essential for both secondary septum formation and the primary septum structural strength needed to counter cell turgor pressure during cell separation.

Cited by 86 publications

References 56 publications

A role for F-BAR protein Rga7p during cytokinesis in S. pombe

A role for F-BAR protein Rga7p during cytokinesis in S. pombe

Negative Functional Interaction Between Cell Integrity MAPK Pathway and Rho1 GTPase in Fission Yeast

Antifungal compound honokiol triggers oxidative stress responsive signalling pathway and modulates central carbon metabolism

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