1998
DOI: 10.1083/jcb.143.7.1775
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Fission Yeast Bub1 Is a Mitotic Centromere Protein Essential for the Spindle Checkpoint and the Preservation of Correct Ploidy through Mitosis

Abstract: The spindle checkpoint ensures proper chromosome segregation by delaying anaphase until all chromosomes are correctly attached to the mitotic spindle. We investigated the role of the fission yeast bub1 gene in spindle checkpoint function and in unperturbed mitoses. We find that bub1 + is essential for the fission yeast spindle checkpoint response to spindle damage and to defects in centromere function. Activation of the checkpoint results in the recruitment of Bub1 to centromeres and a delay in the completion … Show more

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Cited by 152 publications
(131 citation statements)
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“…Fission yeast Mad2p was also independently identified based on its interaction with an activator of the Anaphase Promoting Complex/Cyclosome (APC/C), Slp1p [Kim et al, 1998], which is called Cdc20p in budding yeast and animal cells. S. pombe Mad1p, Mad3p, Bub1p, and Bub3p were identified based on their amino acid sequence similarity to S. cerevisiae proteins [Bernard et al, 1998;Ikui et al, 2002;Kim et al, 2003;. The S. pombe Aurora kinase, Ark1p [Petersen and Hagan, 2003], is required for microtubule-kinetochore attachment and for SAC activation in response to microtubule destabilization.…”
Section: Fission Yeastmentioning
confidence: 99%
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“…Fission yeast Mad2p was also independently identified based on its interaction with an activator of the Anaphase Promoting Complex/Cyclosome (APC/C), Slp1p [Kim et al, 1998], which is called Cdc20p in budding yeast and animal cells. S. pombe Mad1p, Mad3p, Bub1p, and Bub3p were identified based on their amino acid sequence similarity to S. cerevisiae proteins [Bernard et al, 1998;Ikui et al, 2002;Kim et al, 2003;. The S. pombe Aurora kinase, Ark1p [Petersen and Hagan, 2003], is required for microtubule-kinetochore attachment and for SAC activation in response to microtubule destabilization.…”
Section: Fission Yeastmentioning
confidence: 99%
“…However, budding yeast Bub1p and Bub3p [Gillett et al, 2004] and fission yeast Bub1p [Bernard et al, 1998], Bub3p [Vanoosthuyse et al, 2004], and Mad3p localize to kinetochores during every mitosis, and S. cerevisiae bub1 and bub3 mutants [Pangilinan and Spencer 1996;Warren et al, 2002] and S. pombe bub1D mutants [Bernard et al, 1998] undergo chromosome mis-segregation and genetic loss at high frequency during mitosis. In addition, budding yeast Mad1p and Mad2p [Shonn et al, 2000] and fission yeast Mad2p and Bub1p [Bernard et al, 2001] are required for accurate chromosome segregation during meiosis and for spore viability.…”
Section: The Spindle Checkpoint Is Required For Accurate Chromosome Smentioning
confidence: 99%
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“…Spindle checkpoint defects are associated with genetic instability, and some human cancers contain mutant spindle checkpoint genes (Cahill et al 1998;Takahashi et al 1999) The spindle checkpoint monitors the interaction between kinetochores and microtubules. Spindle checkpoint proteins localize to kinetochores (Chen et al 1996;Taylor and McKeon 1997;Bernard et al 1998), and all known kinetochore and spindle defects that activate the checkpoint affect the interaction between kinetochores and microtubules (Wang and Burke 1995;Pangilinan and Spencer 1996;Wells and Murray 1996;Hardwick et al 2000). How does the checkpoint monitor kinetochore alignment?…”
mentioning
confidence: 99%
“…8). Bub1 is a spindle checkpoint protein kinase, which binds to kinetochores every prometaphase in fission yeast 9,10 and is necessary for the localisation of several other checkpoint components on kinetochores in fission yeast and Xenopus, 11,12 and Hardwick unpublished observations). Bub1 clearly performs other functions and among them is the maintenance of sister-chromatid cohesion in meiosis I to prevent equational segregation.…”
mentioning
confidence: 99%