Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis

Kanai, Masayuki; Kume, Kazunori; Miyahara, Katsunori; Sakai, Keisuke; Nakamura, Keigo; Leonhard, Klaus; Wiley, David J.; Verde, Fulvia; Toda, Takashi; Hirata, Dai

doi:10.1038/sj.emboj.7600782

Cited by 67 publications

(115 citation statements)

References 53 publications

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“…Pmo25 is essential for Orb6 kinase activity. The localization of Pmo25 at the SPBs and the kinase activities of Nak1-Orb6 during interphase are under the control of the Cdc7 and Sid1 SIN kinases, suggesting a functional linkage between SIN and the network for cell morphogenesis (Kanai et al 2005). The antagonistic role of Etd1 and PP2A-Pab1 in SIN regulation described in this study represents a new connection between cytokinesis and morphogenesis.…”

Section: Resultsmentioning

confidence: 83%

Antagonistic Roles of PP2A-Pab1 and Etd1 in the Control of Cytokinesis in Fission Yeast

et al. 2010

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In Schizosaccharomyces pombe, Etd1 is a positive regulator of the septation initiation network (SIN), a conserved GTPase-regulated kinase cascade that triggers cytokinesis. Here we show that a mutation in the pab1 gene, which encodes the B-regulatory subunit of the protein phosphatase 2A (PP2A), suppresses mutations in the etd1 gene. Etd1 is required for the function of the GTPase Spg1, a key regulator of SIN signaling. Interestingly, the loss of Pab1 function restored the activity of Spg1 in Etd1-deficient cells. This result suggests that PP2A-Pab1-mediated dephosphorylation inhibits Spg1, thus antagonizing Etd1 function. The loss of pab1 function also rescues the lethality of mutants of other genes in the SIN cascade such as mob1, sid1, and cdc11. Two-hybrid assays indicate that Pab1 physically interacts with Mob1, Sid1, Sid2, and Cdc11, suggesting that the phosphatase 2A B-subunit is a component of the SIN complex. Together, our results indicate that PP2A-Pab1 plays a novel role in cytokinesis, regulating SIN activity at different levels. Pab1 is also required to activate polarized cell growth. Thus, PP2A-Pab1 may be involved in coordinating polar growth and cytokinesis.

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Section: Resultsmentioning

confidence: 83%

Antagonistic Roles of PP2A-Pab1 and Etd1 in the Control of Cytokinesis in Fission Yeast

et al. 2010

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“…Pmo25p, Nak1p and Sog2p localise to the SPB and the division site, whereas Mob2p, Orb6p and Mor2p only localise to the cell periphery and the division site. Orb6p and Nak1p activity is high in interphase and decreases during mitosis, when tip growth ceases (Kanai et al, 2005;Mendoza et al, 2005;Ray et al, 2010).…”

Section: Targets Of Sid2p In the Car Checkpointmentioning

confidence: 99%

“…Its main components are two protein kinase modules, the protein kinase Nak1p (Huang et al, 2003;Leonhard and Nurse, 2005) and its regulators Sog2p (also known as Lrp1p) (Gupta et al, 2013;Kume et al, 2013) and Pmo25p (Kanai et al, 2005;Kume et al, 2007;Mendoza et al, 2005), which act upstream of the kinase Orb6p (Verde et al, 1998) and its regulator Mob2p (Hou et al, 2003). MOR signalling also requires the scaffold protein Mor2p (Hirata et al, 2002).…”

Section: Targets Of Sid2p In the Car Checkpointmentioning

confidence: 99%

Pombe's thirteen – control of fission yeast cell division by the septation initiation network

Simanis

2015

Journal of Cell Science

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The septation initiation network (SIN) regulates aspects of cell growth and division in Schizosaccharomyces pombe and is essential for cytokinesis. Insufficient signalling results in improper assembly of the contractile ring and failure of cytokinesis, generating multinucleated cells, whereas too much SIN signalling uncouples cytokinesis from the rest of the cell cycle. SIN signalling is therefore tightly controlled to coordinate cytokinesis with chromosome segregation. Signalling originates from the cytoplasmic face of the spindle pole body (SPB), and asymmetric localisation of some SIN proteins to one of the two SPBs during mitosis is important for regulation of the SIN. Recent studies have identified in vivo substrates of the SIN, which include components involved in mitotic control, those of the contractile ring and elements of the signalling pathway regulating polarised growth. The SIN is also required for spore formation following meiosis. This has provided insights into how the SIN performs its diverse functions in the cell cycle and shed new light on its regulation.

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“…The NDR-Mob2 complex is activated by a germinal center kinase called Kic1 in S. cerevisiae (Nelson et al, 2003), Nak1 in S. pombe (Huang et al, 2003), and Pod6 in N. crassa (Seiler et al, 2006). Additional elements are Hym1/Mo25-related (Karos and Fischer, 1999;Nelson et al, 2003) and Tao3/ Mor2/Fry-related scaffold-like proteins (Du and Novick, 2002;Kanai et al, 2005). What made this pathway extremely interesting is the fact that in spite of such a broad conservation in their elements, abrogation of this pathway has different morphological outcome depending on the fungi.…”

Section: Introductionmentioning

confidence: 99%

The distinct wiring between cell cycle regulation and the widely conserved Morphogenesis-Related (MOR) pathway in the fungus Ustilago maydis determines the morphological outcome

Sartorel

Pérez-Martı́n

2012

Journal of Cell Science

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SummaryThe morphogenesis-related NDR kinase (MOR) pathway regulates morphogenesis in fungi. In spite of the high conservation of its components, impairing their functions results in highly divergent cellular responses depending on the fungal species. The reasons for such differences are unclear. Here we propose that the species-specific connections between cell cycle regulation and the MOR pathway could be partly responsible for these divergences. We based our conclusion on the characterization of the MOR pathway in the fungus Ustilago maydis. Each gene that encodes proteins of this pathway in U. maydis was deleted. All mutants exhibited a constitutive hyperpolarized growth, contrasting with the loss of polarity observed in other fungi. Using a conditional allele of the central NDR kinase Ukc1, we found that impairing MOR function resulted in a prolonged G2 phase. This cell cycle delay appears to be the consequence of an increase in Cdk1 inhibitory phosphorylation. Strikingly, prevention of the inhibitory Cdk1 phosphorylation abolished the hyperpolarized growth associated with MOR pathway depletion. We found that the prolonged G2 phase resulted in higher levels of expression of crk1, a conserved kinase that promotes polar growth in U. maydis. Deletion of crk1 also abolished the dramatic activation of polar growth in cells lacking the MOR pathway. Taken together, our results suggest that Cdk1 inhibitory phosphorylation may act as an integrator of signaling cascades regulating fungal morphogenesis and that the distinct morphological response observed in U. maydis upon impairment of the MOR pathway could be due to a cell cycle deregulation.

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Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis

Cited by 67 publications

References 53 publications

Antagonistic Roles of PP2A-Pab1 and Etd1 in the Control of Cytokinesis in Fission Yeast

Antagonistic Roles of PP2A-Pab1 and Etd1 in the Control of Cytokinesis in Fission Yeast

Pombe's thirteen – control of fission yeast cell division by the septation initiation network

The distinct wiring between cell cycle regulation and the widely conserved Morphogenesis-Related (MOR) pathway in the fungus Ustilago maydis determines the morphological outcome

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