Fission yeast MOZART1/Mzt1 is an essential γ-tubulin complex component required for complex recruitment to the microtubule organizing center, but not its assembly

Masuda, Hisashi; Mori, Risa; Yukawa, Masashi; Toda, Takashi

doi:10.1091/mbc.e13-05-0235

Cited by 52 publications

(81 citation statements)

References 95 publications

(167 reference statements)

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“…These functions are distinct from the previously described role of GIPs in the recruitment of γ-tubulin complexes (8). Interestingly, the recently characterized GIP homolog, MZT1 in S. pombe, was shown to have two different functions: one for the stabilization of mitotic spindle MTs and the other for the proper segregation of chromosomes without affecting spindle MTs (11). Our data are consistent with these observations because centromeric cohesion was strongly perturbed in gip mutants, whereas centromeres remained cohesive in mad mutants affected in the spindle assembly checkpoint.…”

Section: Gips As a Cornerstone Of Centromere Regulation At The Nuclearmentioning

confidence: 63%

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Arabidopsis MZT1 homologs GIP1 and GIP2 are essential for centromere architecture

Batzenschlager

Lermontová

Schubert

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Centromeres play a pivotal role in maintaining genome integrity by facilitating the recruitment of kinetochore and sister-chromatid cohesion proteins, both required for correct chromosome segregation. Centromeres are epigenetically specified by the presence of the histone H3 variant (CENH3). In this study, we investigate the role of the highly conserved γ-tubulin complex protein 3-interacting proteins (GIPs) in Arabidopsis centromere regulation. We show that GIPs form a complex with CENH3 in cycling cells. GIP depletion in the gip1gip2 knockdown mutant leads to a decreased CENH3 level at centromeres, despite a higher level of Mis18BP1/KNL2 present at both centromeric and ectopic sites. We thus postulate that GIPs are required to ensure CENH3 deposition and/or maintenance at centromeres. In addition, the recruitment at the centromere of other proteins such as the CENP-C kinetochore component and the cohesin subunit SMC3 is impaired in gip1gip2. These defects in centromere architecture result in aneuploidy due to severely altered centromeric cohesion. Altogether, we ascribe a central function to GIPs for the proper recruitment and/or stabilization of centromeric proteins essential in the specification of the centromere identity, as well as for centromeric cohesion in somatic cells.centromere assembly | centromeric cohesion | ploidy stability | Arabidopsis | MZT1

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Section: Gips As a Cornerstone Of Centromere Regulation At The Nuclearmentioning

confidence: 63%

“…This function seems conserved in the human and Schizosaccharomyces pombe GIP homologs named mitotic spindle organizing protein 1 (MZT1) (9)(10)(11). More recently, we localized GIPs at the nucleoplasm periphery, close to chromocenters, where they modulate the nuclear architecture (12,13).…”

mentioning

confidence: 99%

Arabidopsis MZT1 homologs GIP1 and GIP2 are essential for centromere architecture

Batzenschlager

Lermontová

Schubert

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…In S. cerevisiae, only two proteins, Spc97 (GCP2) and Spc98 (GCP3), assemble with ␥-tubulin and form the ␥-tubulin small complex (␥-TuSC). The ␥-tubulin complex in S. pombe, A. nidulans, and higher eukaryotes more resembles the human protein complex, with GCP4-6 and Mzt1 as additional proteins (118)(119)(120)(121). The larger ␥-tubulin protein complex is called the ␥-tubulin ring complex (␥-TuRC).…”

Section: The Fungal Cytoskeleton the Microtubule Cytoskeletonmentioning

confidence: 99%

Fungal Morphogenesis, from the Polarized Growth of Hyphae to Complex Reproduction and Infection Structures

Riquelme

Aguirre

Bartnicki-García

et al. 2018

Microbiol Mol Biol Rev

288

246

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SUMMARYFilamentous fungi constitute a large group of eukaryotic microorganisms that grow by forming simple tube-like hyphae that are capable of differentiating into more-complex morphological structures and distinct cell types. Hyphae form filamentous networks by extending at their tips while branching in subapical regions. Rapid tip elongation requires massive membrane insertion and extension of the rigid chitin-containing cell wall. This process is sustained by a continuous flow of secretory vesicles that depends on the coordinated action of the microtubule and actin cytoskeletons and the corresponding motors and associated proteins. Vesicles transport cell wall-synthesizing enzymes and accumulate in a special structure, the Spitzenkörper, before traveling further and fusing with the tip membrane. The place of vesicle fusion and growth direction are enabled and defined by the position of the Spitzenkörper, the so-called cell end markers, and other proteins involved in the exocytic process. Also important for tip extension is membrane recycling by endocytosis via early endosomes, which function as multipurpose transport vehicles for mRNA, septins, ribosomes, and peroxisomes. Cell integrity, hyphal branching, and morphogenesis are all processes that are largely dependent on vesicle and cytoskeleton dynamics. When hyphae differentiate structures for asexual or sexual reproduction or to mediate interspecies interactions, the hyphal basic cellular machinery may be reprogrammed through the synthesis of new proteins and/or the modification of protein activity. Although some transcriptional networks involved in such reprogramming of hyphae are well studied in several model filamentous fungi, clear connections between these networks and known determinants of hyphal morphogenesis are yet to be established.

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“…A conserved small polypeptide (approximately 8 kD), dubbed the Mitotic-spindle organizing protein associated with a ring of gtubulin 1 (MOZART1; At4g09550 and At1g73790), interacts with the gTuRC via GCP3 (At5g06680) and is required for MT nucleation in fission yeast (Masuda et al, 2013;Dhani et al, 2013), human (Hutchins et al, 2010), and Arabidopsis (Janski et al, 2012;Nakamura et al, 2012). In fission yeast, the core gTuRC still assembles in the absence of MOZART1, indicating that it is a peripheral regulatory component of gTuRC.…”

Section: Mt Nucleationmentioning

confidence: 99%

Microtubules in Plants

Hashimoto

2015

The Arabidopsis Book

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Microtubules (MTs) are highly conserved polar polymers that are key elements of the eukaryotic cytoskeleton and are essential for various cell functions. αβ-tubulin, a heterodimer containing one structural GTP and one hydrolysable and exchangeable GTP, is the building block of MTs and is formed by the sequential action of several molecular chaperones. GTP hydrolysis in the MT lattice is mechanistically coupled with MT growth, thus giving MTs a metastable and dynamic nature. MTs adopt several distinct higher-order organizations that function in cell division and cell morphogenesis. Small molecular weight compounds that bind tubulin are used as herbicides and as research tools to investigate MT functions in plant cells. The de novo formation of MTs in cells requires conserved γ-tubulin-containing complexes and targeting/activating regulatory proteins that contribute to the geometry of MT arrays. Various MT regulators and tubulin modifications control the dynamics and organization of MTs throughout the cell cycle and in response to developmental and environmental cues. Signaling pathways that converge on the regulation of versatile MT functions are being characterized.

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Fission yeast MOZART1/Mzt1 is an essential γ-tubulin complex component required for complex recruitment to the microtubule organizing center, but not its assembly

Cited by 52 publications

References 95 publications

Arabidopsis MZT1 homologs GIP1 and GIP2 are essential for centromere architecture

Arabidopsis MZT1 homologs GIP1 and GIP2 are essential for centromere architecture

Fungal Morphogenesis, from the Polarized Growth of Hyphae to Complex Reproduction and Infection Structures

Microtubules in Plants

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