FITC Labeled Silica Nanoparticles as Efficient Cell Tags: Uptake and Photostability Study in Endothelial Cells

Veeranarayanan, Srivani; Poulose, Aby Cheruvathoor; Mohamed, M. Sheikh; Aravind, Athulya; Nagaoka, Yutaka; Yoshida, Yasuhiko; Maekawa, Toru; Kumar, D. Sakthi

doi:10.1007/s10895-011-0991-3

Cited by 44 publications

(37 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…b Cell lines treated with non-targeted Si-PTX NPs-all cell lines were dead proving non-specificity of nanomaterials devoid of targeting scheme. c Cell lines treated with Apt-Si-PTX NPsnormal L929 cells were spared and cancer cell lines were 90% dead microscopy showed the internalization of Si-FITC nanoparticles by both the plants cells as well as animal cells in our previous reports [13,14]. Our earlier report showed that 2 h incubation was quite enough for internalization of silica nanoparticles inside live cells.…”

Section: Resultsmentioning

confidence: 80%

“…Silica nanoparticles were prepared using template-directed synthesis method that has been reported elsewhere [14]. CTAB was used as template for pore formation, TEOS as silica source, ammonia as catalyst along with ethanol, and dd.H 2 O as solvent.…”

Section: Preparation Of Porous Silicamentioning

confidence: 99%

“…Functionalizing these silica particles with biomolecules activates the molecular recognition property of the particles enabling targeted drug delivery [12]. Our previous study reported on the efficiency of FITC-loaded silica nanoparticles in maintaining their photo stability in plants and animal cells [13,14].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Aptamer-Functionalized Silica Nanoparticles for Targeted Cancer Therapy

et al. 2011

Self Cite

View full text Add to dashboard Cite

Target-specific drug delivery system that can transport an effective dosage of anti-cancer drugs to the targeted tumor cells can significantly reduce drug toxicity to the normal cells and increase the therapeutic effect of the drug. In our work, we have evaluated the cytotoxic potential of paclitaxel-loaded silica nanoparticles (Si-PTX NPs) prepared by template-directed stöber synthesis technique. The particles were characterized using transmission electron microscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy. We have modified the surface of the drug-loaded particles chemically and conjugated a tumor-specific aptamer (Apt-Si-PTX NPs) to facilitate targeted drug delivery to the cancer cells. In vitro studies carried out demonstrated that the aptamerconjugated paclitaxel-loaded silica nanoparticles could target the cancer cells with high specificity and destroy them effectively, while sparing the normal cells. This work concludes that the aptamer-tagged paclitaxel-loaded silica nanoparticles are excellent targeting moieties for targeted drug delivery to tumor cells.

show abstract

Section: Resultsmentioning

confidence: 80%

Section: Preparation Of Porous Silicamentioning

confidence: 99%

See 1 more Smart Citation

Aptamer-Functionalized Silica Nanoparticles for Targeted Cancer Therapy

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, silica is an inert layer that confers water solubility and shields the optical property of the core. [34][35][36][37][38] Cadmium selenide and cadmium telluride QDs have been widely synthesized and studied as cyto-labels. [39][40][41] Cadmium sulfide (CdS) QDs have, as of yet, not created their own niche in the area of biological imaging due to their weak emissions in comparison with their selenide and telluride counterparts.…”

Section: Preparation Of Bare Cds and Silica-coated Cdsmentioning

confidence: 99%

Synthesis and application of luminescent single CdS quantum dot encapsulated silica nanoparticles directed for precision optical bioimaging

Veeranarayanan¹,

Poulose²,

Mohamed³

et al. 2012

IJN

Self Cite

View full text Add to dashboard Cite

This paper presents the synthesis of aqueous cadmium sulfide (CdS) quantum dots (QDs) and silica-encapsulated CdS QDs by reverse microemulsion method and utilized as targeted bio-optical probes. We report the role of CdS as an efficient cell tag with fluorescence on par with previously documented cadmium telluride and cadmium selenide QDs, which have been considered to impart high levels of toxicity. In this study, the toxicity of bare QDs was efficiently quenched by encapsulating them in a biocompatible coat of silica. The toxicity profile and uptake of bare CdS QDs and silica-coated QDs, along with the CD31-labeled, silica-coated CdS QDs on human umbilical vein endothelial cells and glioma cells, were investigated. The effect of size, along with the time-dependent cellular uptake of the nanomaterials, has also been emphasized. Enhanced, high-specificity imaging toward endothelial cell lines in comparison with glioma cells was achieved with CD31 antibody-conjugated nanoparticles. The silica-coated nanomaterials exhibited excellent biocompatibility and greater photostability inside live cells, in addition to possessing an extended shelf life. In vivo biocompatibility and localization study of silica-coated CdS QDs in medaka fish embryos, following direct nanoparticle exposure for 24 hours, authenticated the nanomaterials' high potential for in vivo imaging, augmented with superior biocompatibility. As expected, CdS QD-treated embryos showed 100% mortality, whereas the silica-coated QD-treated embryos stayed viable and healthy throughout and after the experiments, devoid of any deformities. We provide highly cogent and convincing evidence for such silica-coated QDs as a model nanoparticle in practice, to achieve in vitro and in vivo precision targeted imaging.

show abstract

“…Cancer nanotechnology has become a well researched interdisciplinary field enabling fabrication of nanotools to yield high image contrast, sensitivity and specificity [5][6][7]. Recent research has witnessed wide range of fluorescent nanomaterials applied for biological imaging [8]. Fluorescent markers at single molecular level are the need of the hour in particular for imaging molecular events inside live cells, such as signaling pathways for the study of cellular metabolism [9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

PEG Coated Biocompatible Cadmium Chalcogenide Quantum Dots for Targeted Imaging of Cancer Cells

et al. 2012

Self Cite

View full text Add to dashboard Cite

Cancer stands as a leading cause of mortality worldwide and diagnostics of cancer still faces drawbacks. Optical imaging of cancer would allow early diagnosis, evaluation of disease progression and therapy efficiency. To that aim, we have developed highly biocompatible PEG functionalized cadmium chalcogenide based three differently luminescent quantum dots (QDs) (CdS, CdSe and CdTe). Folate targeting scheme was utilized for targeting cancer cell line, MCF-7. We demonstrate the biocompatibility, specificity and efficiency of our nanotool in detection of cancer cells sparing normal cell lines with retained fluorescence of functionalized QDs as parental counterpart. This is the first time report of utilizing three differently fluorescent QDs and we have detailed about the internalization of these materials and time dependent saturation of targeting schemes. We present here the success of utilizing our biocompatible imaging tool for early diagnosis of cancer.

show abstract

FITC Labeled Silica Nanoparticles as Efficient Cell Tags: Uptake and Photostability Study in Endothelial Cells

Cited by 44 publications

References 25 publications

Aptamer-Functionalized Silica Nanoparticles for Targeted Cancer Therapy

Aptamer-Functionalized Silica Nanoparticles for Targeted Cancer Therapy

Synthesis and application of luminescent single CdS quantum dot encapsulated silica nanoparticles directed for precision optical bioimaging

PEG Coated Biocompatible Cadmium Chalcogenide Quantum Dots for Targeted Imaging of Cancer Cells

Contact Info

Product

Resources

About