Fitness costs in chlorfenapyr-resistant populations of the chive maggot, Bradysia odoriphaga

Ullah, Farman; Gül, Hina; Desneux, Nicolas; Said, Fazal; Gao, Xiwu; Song, Dunlun

doi:10.1007/s10646-020-02183-7

Cited by 30 publications

(25 citation statements)

References 73 publications

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“…Due to excellent insecticidal activity, clothianidin has been broadly used against many insect pests including hemipterans and many species of beetles [ 8 , 28 – 33 ]. However, the insecticidal actions have been dramatically affected by the development of insecticide resistance [ 15 , 43 , 45 ]. There have been numerous studies to investigate the development of insecticide resistance against various pests such as Chive maggot, Colorado potato beetle, small brown plant hopper, western flower thrips, peach aphid and tobacco aphid [ 15 , 45 , 59 – 62 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, the insecticidal actions have been dramatically affected by the development of insecticide resistance [ 15 , 43 , 45 ]. There have been numerous studies to investigate the development of insecticide resistance against various pests such as Chive maggot, Colorado potato beetle, small brown plant hopper, western flower thrips, peach aphid and tobacco aphid [ 15 , 45 , 59 – 62 ]. The resistance development accompanied by fitness costs significantly affect the evolution of insecticide resistance [ 43 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…There have been numerous studies to investigate the development of insecticide resistance against various pests such as Chive maggot, Colorado potato beetle, small brown plant hopper, western flower thrips, peach aphid and tobacco aphid [ 15 , 45 , 59 – 62 ]. The resistance development accompanied by fitness costs significantly affect the evolution of insecticide resistance [ 43 , 45 ]. To our knowledge, no previous research has investigated the development of clothianidin resistance and associated fitness costs in the melon aphid, A .…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies by Ullah et al and Gul et al concluded 43.32- and 76-fold resistance in B . odoriphaga following 10 consecutive generations selection to chlorfenapyr and clothianidin, respectively [ 15 , 45 ]. Beet armyworm, Spodoptera exigua Hübner (Lepidoptera: Noctuidae) showed 69.76- and 113.29-fold resistance against deltamethrin and gossypol, respectively following 10 generations selections [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

“…The life-history traits of B . odoriphaga have significantly been affected in clothianidin and chlorfenapyr resistant populations compared to the susceptible strain [ 15 , 45 ]. Several studies have reported the development of insecticide resistance accompanied by fitness costs in different insect pests [ 15 , 46 – 49 ].…”

Section: Introductionmentioning

confidence: 99%

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Fitness costs in clothianidin-resistant population of the melon aphid, Aphis gossypii

et al. 2020

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Clothianidin is a second-generation neonicotinoid insecticide, widely used against sap-sucking insect pest including melon aphid, Aphis gossypii Glover (Hemiptera: Aphididae). This pest causes severe economic damage to Cucurbitaceae plants worldwide. In this study, we investigated clothianidin resistance development under continuous selection pressure. Moreover, the age-stage, two-sex life table approach was used to evaluate the impact of clothianidin resistance on the fitness of A . gossypii . A clothianidin resistant strain (CT-R) with a 23.17-fold resistance level was developed from a susceptible strain (CT-S) after continuous selection for 24 generations. Life table results showed a significant reduction in the relative fitness (0.847) of CT-R strain compared to the CT-S strain of A . gossypii . The developmental duration, oviposition days, total pre-oviposition period (TPOP), longevity, and fecundity of CT-R strain were found to be significantly lower when compared to CT-S strain. The demographic parameters, including the intrinsic rate of increase ( r ), finite rate of increase ( λ ), net reproductive rate ( R 0 ), and mean generation time ( T ) were also significantly decreased in CT-R strain compared to the CT-S strain. Both the reproductive and survival rates were affected by clothianidin resistance in CT-R strain compared with the CT-S strain of A . gossypii . Overall, our results demonstrate that in-depth knowledge about the trade-off at play between resistance degree and fitness cost might be useful to design resistance management strategies against A . gossypii .

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fitness costs in clothianidin-resistant population of the melon aphid, Aphis gossypii

et al. 2020

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Functional investigation of lncRNAs and target cytochrome P450 genes related to spirotetramat resistance in Aphis gossypii Glover

Peng

Liu

Tian

et al. 2022

Pest Management Science

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BACKGROUND Spirotetramat is a tetramic acid derivative insecticide with novel modes of action for controlling Aphis gossypii Glover in the field. Previous studies have shown that long noncoding RNAs (lncRNAs) and cytochrome P450 monooxygenases (P450s) are involved in the detoxification process. However, the functions of lncRNAs in regulating P450 gene expression in spirotetramat resistance in A. gossypii are unknown. RESULTS In this study, we found CYP4CJ1, CYP6CY7 and CYP6CY21 expression levels to be significantly upregulated in a spirotetramat‐resistant (SR) strain compared with a susceptible (SS) strain. Furthermore, knockdown of CYP4CJ1, CYP6CY7 and CYP6CY21 increased nymph and adult mortality in the SR strain following exposure to spirotetramat. Drosophila ectopically expressing CYP380C6, CYP4CJ1, CYP6DA2, CYP6CY7 and CYP6CY21 showed significantly decreased mortality after spirotetramat exposure, and CYP380C6, CYP4CJ1 and CYP6CY21 are putative targets of six lncRNAs. Silencing of lncRNAs MSTRG.36649.2/5 and MSTRG.71880.1 changed CYP6CY21 and CYP380C6 expression, altering the sensitivity of the SR strain to spirotetramat. Moreover, MSTRG.36649.2/5 did not compete for microRNA (miRNA) binding to regulate CYP6CY21 expression. CONCLUSION Our results confirm that CYP380C6, CYP4CJ1, CYP6DA2, CYP6CY7 and CYP6CY21 are potentially involved in the development of spirotetramat resistance in A. gossypii, and MSTRG.36649.2/5 and MSTRG.71880.1 probably regulate CYP6CY21 and CYP380C6 expression other than through the “sponge effect” of competing for miRNA binding. Our results provide a favorable molecular basis for studying cotton aphid P450 genes and lncRNA functions in spirotetramat resistance development.

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Characterization of two glutathione S‐transferase genes involved in clothianidin resistance in Bradysia odoriphaga

Ma,

Zeng,

Zhang

et al. 2024

Pest Management Science

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BACKGROUNDGlutathione S‐transferase (GST) is a key phase II detoxification enzyme involved in xenobiotics metabolism, and plays a pivotal role in the evolution of resistance to various types of insecticides. However, the specific functions of GST genes in clothianidin resistance remain obscure in Bradysia odoriphaga.RESULTSHere, a specific GST inhibitor, diethyl maleate (DEM), significantly increased the mortality of Bradysia odoriphaga larvae following exposure to clothianidin, and the activity of GST enzyme in clothianidin‐resistant (CL‐R) strain of Bradysia odoriphaga was markedly greater than that in the SS strain. Two sigma BoGSTs (BoGSTs1 and BoGSTs2) were markedly overexpressed in the CL‐R strain and exhibited a higher abundance in the Malpighian tubules or midgut. Exposure to clothianidin resulted in a significant increased expression of BoGSTs1 and BoGSTs2. The knockdown of BoGSTs1 and BoGSTs2 increased sensitivity of larvae to clothianidin in the resistant strain. Furthermore, overexpression of BoGSTs1 and BoGSTs2 led to a significant increase in Escherichia coli cells tolerance to clothianidin. In vitro metabolic assays indicate that these two GSTs cannot directly metabolize clothianidin and its secondary metabolite desmethyl‐clothianidin. Disk diffusion assays and fluorescence competitive binding assays indicated that BoGSTs1 and BoGSTs2 play a critical role in clothianidin resistance by antioxidant activity and non‐catalytic binding activity. The docking results showed that BoGSTs1 and BoGSTs2 have strong binding affinity toward clothianidin.CONCLUSIONCollectively, these findings pinpoint the potential role of BoGSTs1 and BoGSTs2 in conferring insecticide resistance in Bradysia odoriphaga and contribute to our understanding of the underlying mechanisms of insecticide resistance. © 2024 Society of Chemical Industry.

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Fitness costs in chlorfenapyr-resistant populations of the chive maggot, Bradysia odoriphaga

Cited by 30 publications

References 73 publications

Fitness costs in clothianidin-resistant population of the melon aphid, Aphis gossypii

Fitness costs in clothianidin-resistant population of the melon aphid, Aphis gossypii

Functional investigation of lncRNAs and target cytochrome P450 genes related to spirotetramat resistance in Aphis gossypii Glover

Characterization of two glutathione S‐transferase genes involved in clothianidin resistance in Bradysia odoriphaga

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