Five miRNAs as Novel Diagnostic Biomarker Candidates for Primary Nasopharyngeal Carcinoma

Tang, Jingfeng; Zhong, Yu; Li, Shipeng; Lin, Ziying; Wang, Yahong; Yang, Liting; He, Huijuan; Cao, Jun; Huang, Haili; Liu, Gang

doi:10.7314/apjcp.2014.15.18.7575

Cited by 31 publications

(21 citation statements)

References 38 publications

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“…Family members of the miR-183 are proposed as promising biomarkers for early cancer detection, prognosis, and as therapeutic targets in several cancers [ 65 - 67 ]. However, the results of their expression profiling in cancer tissues are inconsistent and controversial (Reviewed in [ 68 ]).…”

Section: Discussionmentioning

confidence: 99%

Circulating small non coding RNA signature in head and neck squamous cell carcinoma

et al. 2015

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The Head and Neck Squamous Cell Carcinoma (HNSCC) is the sixth most common human cancer, causing 350,000 individuals die worldwide each year. The overall prognosis in HNSCC patients has not significantly changed for the last decade. Complete understanding of the molecular mechanisms in HNSCC carcinogenesis could allow an earlier diagnosis and the use of more specific and effective therapies. In the present study we used deep sequencing to characterize small non-coding RNAs (sncRNAs) in serum from HNSCC patients and healthy donors. We identified, for the first time, a multi-marker signature of 3 major classes of circulating sncRNAs in HNSCC, revealing the presence of circulating novel and known miRNAs, and tRNA- and YRNA-derived small RNAs that were significantly deregulated in the sera of HNSCC patients compared to healthy controls. By implementing a triple-filtering approach we identified a subset of highly biologically relevant miRNA-mRNA interactions and we demonstrated that the same genes/pathways affected by somatic mutations in cancer are affected by changes in the abundance of miRNAs. Therefore, one important conclusion from our work is that during cancer development, there seems to be a convergence of oncogenic processes driven by somatic mutations and/or miRNA regulation affecting key cellular pathways.

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Section: Discussionmentioning

confidence: 99%

Circulating small non coding RNA signature in head and neck squamous cell carcinoma

et al. 2015

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“…miRNAs constitute a family of small non-coding RNAs that are approximately 22 nucleotides in length [5], and these molecules can play oncogenic or tumor suppressor roles during tumorigenesis by influencing malignant biological behaviors such as proliferation, apoptosis, radioresistance, chemoresistance, and metastasis [6][7][8][9]. miR-93-5p, an miRNA within the miR-106b- 25 cluster, has been reported to be dysregulated in various types of cancer such as cervical, breast, and bladder cancers, as well as nasopharyngeal carcinoma [10][11][12][13]. Our previous study demonstrated that miR-93-5p is upregulated in SCCHN tissues, and this upregulation is closely related to the clinicopathological parameters of T status, lymph node metastasis, and clinical stage [14].…”

Section: Ivyspringmentioning

confidence: 99%

miR-93-5p enhances migration and invasion by targeting RGMB in squamous cell carcinoma of the head and neck

Zhang

Liu

et al. 2020

J. Cancer

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Invasion and metastasis represent the primary causes of therapeutic failure in patients diagnosed with squamous cell carcinoma of the head and neck (SCCHN). Therefore, disease prediction and inhibition of invasion and metastasis are critical for enhancing the survival of patients with SCCHN. Our previous study revealed that increased expression of miR-93-5p is associated with poor prognosis in SCCHN; however, the mechanism underlying the oncogenic functions of miR-93-5p in SCCHN migration and invasion remains unclear. Using qPCR analyses, transwell assays, and scratch tests, we demonstrated that expression of ectopic miR-93-5p induced the migration and invasion of SCCHN, and this was accompanied by corresponding alterations in biomarkers and transcription factors specific for epithelial-mesenchymal transition (EMT). Luciferase reporter assays were used to demonstrate that miR-93-5p directly targeted the 3' UTR of RGMB, and we further found that the tumor-promoting functions of miR-93-5p were partly mediated by targeting RGMB, whose downregulation also promoted the migration and invasion of SCCHN. Overall, our results indicate that miR-93-5p acts as an oncogene in the regulation of migration and invasion by suppressing RGMB in SCCHN. These findings provide novel evidence that miR-93-5p may serve as a valuable predictive biomarker and potential intervention target in patients with SCCHN.Key words: miR-93-5p, squamous cell carcinoma of the head and neck, RGMB, epithelial-mesenchymal transition

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“…Two of the upregulated NW miRNAs, hsa-miR-93 and hsa-miR-205 concurred with the findings of other studies. 19,20 Both hsa-miR-93 and hsa-miR-205 were shown to be upregulated in NPC tissue samples 21,22 and could lead to enhance cell growth, migration and invasion in NPC cell lines. 21,23 Meanwhile, hsa-miR-21 and hsa-miR-421 that were shown to be upregulated in NW were consistent with the trend reported by 1 to 2 NPC tissue profiling studies (Supporting Information Table S1).…”

Section: Nw Ebv Dna Load As Biomarker For Npc Detection and Monitoringmentioning

confidence: 99%

A novel and non‐invasive approach utilising nasal washings for the detection of nasopharyngeal carcinoma

Tan

Sivanesan

Rahman

et al. 2019

Intl Journal of Cancer

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Nasopharyngeal carcinoma (NPC) is an epithelial cancer of the nasopharynx which is highly associated with Epstein–Barr virus (EBV). Worldwide, most of the top 20 countries with the highest incidence and mortality rates of NPC are low‐ and middle‐income countries. Many studies had demonstrated that EBV could be detected in the tissue, serum and plasma of NPC patients. In this study, we explored the potential of assays based on non‐invasive nasal washings (NW) as a diagnostic and prognostic tool for NPC. A total of 128 patients were evaluated for NW EBV DNA loads and a subset of these samples were also tested for 27 EBV and human miRNAs shortlisted from literature. EBV DNA and seven miRNAs showed area under the receiver operating characteristic curve (AUC) values of more than 0.7, suggestive of their potential utility to detect NPC. Logistic regression analyses suggested that combination of two NW assays that test for EBNA‐1 and hsa‐miR‐21 had the best performance in detecting NPC. The trend of NW EBV DNA load matched with clinical outcome of 71.4% (10 out of 14) NPC patients being followed‐up. In summary, the non‐invasive NW testing panel may be particularly useful for NPC screening in remote areas where healthcare facilities and otolaryngologists are lacking, and may encourage frequent testing of individuals in the high risk groups who are reluctant to have their blood tested. However, further validation in an independent cohort is required to strengthen the utility of this testing panel as a non‐invasive detection tool for NPC.

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Five miRNAs as Novel Diagnostic Biomarker Candidates for Primary Nasopharyngeal Carcinoma

Cited by 31 publications

References 38 publications

Circulating small non coding RNA signature in head and neck squamous cell carcinoma

Circulating small non coding RNA signature in head and neck squamous cell carcinoma

miR-93-5p enhances migration and invasion by targeting RGMB in squamous cell carcinoma of the head and neck

A novel and non‐invasive approach utilising nasal washings for the detection of nasopharyngeal carcinoma

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