Five novel Kitasatospora species from soil: Kitasatospora arboriphila sp. nov., K. gansuensis sp. nov., K. nipponensis sp. nov., K. paranensis sp. nov. and K. terrestris sp. nov.

Abstract

A polyphasic study was carried out to establish the taxonomic positions of six strains isolated from diverse soil samples and provisionally assigned to the genus Kitasatospora. The isolates were found to have chemical and morphological properties consistent with their classification as Kitasatospora strains. Direct 16S rRNA gene sequence data confirmed the taxonomic position of the strains following the generation of phylogenetic trees by using three tree-making algorithms. Five of the isolates were considered to merit species status using complementary genotypic and phenotypic data. These organisms were designated Kitasatospora arboriphila sp. nov. (HKI 0189(T)=2291-120(T)=DSM 44785(T)=NCIMB 13973(T)), Kitasatospora gansuensis sp. nov. (HKI 0314(T)=2050-015(T)=DSM 44786(T)=NCIMB 13974(T)), Kitasatospora nipponensis sp. nov. (HKI 0315(T)=2148-013(T)=DSM 44787(T)=NCIMB 13975(T)), Kitasatospora paranensis sp. nov. (HKI 0190(T)=2292-041(T)=DSM 44788(T)=NCIMB 13976(T)) and Kitasatospora terrestris sp. nov. (HKI 0186(T)=2293-012(T)=DSM 44789(T)=NCIMB 13977(T)). The remaining organism, isolate HKI 0316 (=2122-022=DSM 44790=NCIMB 13978), was considered to be a strain of Kitasatospora kifunensis on the basis of 16S rRNA gene sequence, DNA-DNA relatedness and phenotypic data.

Salinactinospora qingdaonensis gen. nov., sp. nov., a halophilic actinomycete isolated from a salt pond

“…Tests for hydrolysis of cellulose, gelatin, starch and Tweens 20, 40, 60 and 80, nitrate reduction, utilization of urea, milk coagulation and peptonization and production of H 2 S and melanin were performed with 10 % (w/v) NaCl as described by Gonzalez et al (1978). Antibiotic susceptibility was examined according to Groth et al (2004) using antibiotic discs on ISP 4 agar with 10 % (w/v) NaCl. Haloactinospora alba DSM 45015 T (the type species of the genus Haloactinospora) obtained from the DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany) was used as a reference strain and cultured under the same conditions.…”

Rhodococcus canchipurensis sp. nov., an actinomycete isolated from a limestone deposit site

“…Nitrate reduction was monitored as described by Lányí (1987). Antibiotic susceptibility test was performed as described by Groth et al (2004) using antibiotic discs (HiMedia). Growth at 5,15,28,37,42,50 and 60 u C,at pH 4,5,6,7,8,9 and 10, and with 0, 2, 5, 7 and 10 % NaCl was determined on TSA as described by Goodfellow (1986).…”

Sciscionella marina gen. nov., sp. nov., a marine actinomycete isolated from a sediment in the northern South China Sea

“…Physiological tests including hydrolysis of cellulose, gelatin, starch and Tweens 20, 40, 60 and 80, nitrate reduction, utilization of urea, milk coagulation and peptonization and H 2 S and melanin production were performed as described previously (Gonzalez et al, 1978;Smibert & Krieg, 1981). Antibiotic susceptibility was examined as described by Groth et al (2004) using antibiotic discs on modified ISP 2 medium.…”