Fixation of horseradish peroxidase reaction products with ammonium molybdate

Fujii, Masako; Kusama, Tadashi

doi:10.1016/s0168-0102(84)80012-7

Cited by 68 publications

(15 citation statements)

References 5 publications

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“…Three series of every five sections were collected; one series was processed with tetramethylbenzidine (TMB; Mesulam, 1978) for anterograde and retrograde labeling, the next series was processed with benzidine dihydrochloride (BDHC; Mesulam, 1976) for determining the injection sites, and the other series was processed with Nissl staining for detailed cytoarchitectonic analysis. The reaction products of HRP histochemistry were stabilized with 5% ammonium molybdate for 20 minutes at room temperature (Fujii and Kusama, 1984). After sections were mounted on gelatinized slides and air dried, the first and second series processed with HRP histochemistry were counterstained with neutral red; the third series of unprocessed sections was stained with cresyl violet for Nissl staining.…”

Section: Methodsmentioning

confidence: 99%

Cortical projections of the parvocellular laminae C of the dorsal lateral geniculate nucleus in the cat: An anterograde wheat germ agglutinin conjugated to horseradish peroxidase study

Kawano

1998

J. Comp. Neurol.

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The areal and laminar distributions of the projection from the parvocellular part of laminae C of the dorsal lateral geniculate nucleus (Cparv) were studied in visual cortical areas of the cat with the anterograde tracing method by using wheat germ agglutinin conjugated to horseradish peroxidase. A particular objective of this study was to examine the central visual pathways of the W-cell system, the precise organization of which is still unknown. Because the Cparv in the cat is said to receive W-cell information exclusively from the retina and the superior colliculus, the results obtained would provide an anatomical substrate for the W-cell system organization in mammals. The results show that the cortical targets of the Cparv are areas 17, 18, 19, 20a, and 21a and the posteromedial lateral suprasylvian (PMLS) and ventral lateral suprasylvian(VLS) areas. In area 17, the projection fibers terminate in the superficial half of layer I; the lower two-thirds of layer III, extending to the superficial part of layer IV; and the deep part of layer IV, involving layer Va. These terminations form triple bands in area 17. The projection terminals in layer I are continuous, whereas those in layers III, IV, and Va distribute periodically, exhibiting a patchy appearance. In areas 18 and 19, the projection fibers terminate in the superficial half of layer I and in the full portions of layers III and IV, forming double bands. In these areas, the terminals in layer I are continuous, whereas those in layers III and IV distribute periodically, exhibiting a patchy appearance. In area 20a, area 21a, PMLS, and VLS, projection fibers terminate in the superficial part of layer I, in part of layer III, and in the full portion of layer IV, although they are far fewer in number than those seen in areas 17, 18, and 19. The present results demonstrate that the Cparv fibers terminate in a localized fashion in both the striate and the extrastriate cortical areas and that these W-cell projections are quite unique in their areal and laminar organization compared with the X- and Y-cell systems.

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Section: Methodsmentioning

confidence: 99%

Cortical projections of the parvocellular laminae C of the dorsal lateral geniculate nucleus in the cat: An anterograde wheat germ agglutinin conjugated to horseradish peroxidase study

Kawano

1998

J. Comp. Neurol.

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“…Every alternate section was left unstained for examination with a fluorescence microscope, and was later Nissl-stained with cresyl violet for cytoarchitectonic examination. The remaining half of the sections were processed for HRP staining with tetramethylbenzidine (Mesulam, 1978;Gibson et al, 1984;Fujii and Kusama, 1984).…”

Section: Methodsmentioning

confidence: 99%

Spatial distribution of thalamic projections to the supplementary motor area and the primary motor cortex: A retrograde multiple labeling study in the macaque monkey

Shindo

Shima

Tanji

1995

J of Comparative Neurology

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The exact knowledge on spatial organization of information sources from the thalamus to the supplementary motor area (SMA) and to the primary motor cortex (MI) has not been established. We investigated the distribution of thalamocortical neurons projecting to forelimb representations of the SMA and the MI using a multiple retrograde labeling technique in the monkey. The forelimb area of the SMA, and the distal and proximal forelimb areas of the MI were identified by electrophysiological techniques of intracortical microstimulation and single neuron recording. Injections were made into these three representations with three different dyes in the same animal (horseradish peroxidase conjugated to wheat germ agglutinin, diamidino yellow, and fast blue), and the thalamic neurons were retrogradely labeled. Injections into the SMA densely labeled thalamic neurons in nuclei ventralis lateralis pars oralis (VLo), ventralis lateralis pars medialis (VLm) and ventralis lateralis pars caudalis (VLc), but not in nucleus ventralis posterior lateralis pars oralis (VPLo). Injections into the MI labeled thalamic neurons primarily in VLo, VLc, and VPLo. We found that the distribution of projection neurons to the three areas was largely separate in the thalamus. However, in the middle part of VLo, and in a limited portion of VLc, thalamic neurons projecting to the SMA partially overlapped with those to the distal forelimb area of the MI. They overlapped little with those to the proximal forelimb area of the MI. We noted no overlap between the distributions of thalamic projection neurons to the distal and proximal forelimb areas of the MI. These findings suggest that the SMA and MI receive separate information from the thalamus, while sharing minor sources of common inputs.

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“…One-in-five serial sections were reacted with 3, 3Ј, 5, 5Ј-tetramethylbenzidine (Merck, Darmstadt, Germany) to visualize WGA-HRP labeling (Mesulam, 1978). After the reaction, the sections were processed with 5% ammonium molybdate for 30 minutes to prevent the reaction product from fading (Fujii and Kusama, 1984). These labeled sections were observed, plotted, and photographed under brightfield and polarizing light with a microscope fitted with a camera lucida (Nikon, Tokyo, Japan).…”

Section: Tracer Injectionsmentioning

confidence: 99%

Cytoarchitectonic and connectional organization of the ventral lateral geniculate nucleus in the cat

Nakamura

Itoh

2004

J of Comparative Neurology

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The ventral lateral geniculate nucleus is a small extrageniculate visual structure that has a complex cytoarchitecture and diverse connections. In addition to small-celled medial and lateral divisions, we cytoarchitectonically defined a small-celled dorsal division. A large-celled intermediate division intercalated between the three small-celled divisions, which we divided into medial and lateral intermediate subdivisions. In WGA-HRP injection experiments, the different cytoarchitectonic divisions were shown to have connections with different nuclei. The medial division was reciprocally connected to the pretectum and projected to the superficial layers of the superior colliculus and the intralaminar nuclei. The medial intermediate division received projections from the intermediate layer of the superior colliculus and the lateral and interpositus posterior cerebellar nuclei, and projected to the intermediate layer of the superior colliculus, the periaqueductal gray of midbrain, and the intralaminar nuclei. The lateral intermediate divisions received projections from the pretectum, the intermediate layer of the superior colliculus, and the lateral and interpositus posterior cerebellar nuclei, and projected to the pretectum, superficial layers of the superior colliculus, and the pulvinar. The lateral division received projections from superficial layers of the superior colliculus and had reciprocal connections with the pretectum. The dorsal division received projections from the pretectum and had reciprocal connections with the periaqueductal gray of midbrain. The different cytoarchitectonic divisions of the ventral lateral geniculate nucleus are thus suggested to play different functional roles related to vision, eye and head movements, attention, and defensive reactions.

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Fixation of horseradish peroxidase reaction products with ammonium molybdate

Cited by 68 publications

References 5 publications

Cortical projections of the parvocellular laminae C of the dorsal lateral geniculate nucleus in the cat: An anterograde wheat germ agglutinin conjugated to horseradish peroxidase study

Cortical projections of the parvocellular laminae C of the dorsal lateral geniculate nucleus in the cat: An anterograde wheat germ agglutinin conjugated to horseradish peroxidase study

Spatial distribution of thalamic projections to the supplementary motor area and the primary motor cortex: A retrograde multiple labeling study in the macaque monkey

Cytoarchitectonic and connectional organization of the ventral lateral geniculate nucleus in the cat

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