Fixative and Alcohol-Induced Weight Change in Eggs from the Lizard, Sceloporus undulatus

Guillette, Louis J.; Rand, Matt S.; DeMarco, Vincent G.; Etheridge, Kay

doi:10.2307/1564364

Cited by 5 publications

(3 citation statements)

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“…However, these collections have been fixed and stored for various durations of time-some being stored in ethanol for over 100 years. While fluid preservation has been demonstrated to have some morphological effects on specimens (Berbel-Filho et al, 2013;Bininda-Emonds & Russell, 1994;Buchheister & Wilson, 2005;Cunningham et al, 2000;Deichmann et al, 2009;Gaston et al, 2013;Guillette et al, 1988;Hetherington et al, 2019;Larochelle et al, 2016;Lee, 1982;Moku et al, 2004;Sotola et al, 2019;Thacker & Gkenas, 2019;Vervust et al, 2009;Voight, 2001) these effects have not been systematically quantified in typical museum specimens, until now.…”

Section: Discussionmentioning

confidence: 99%

“…These changes are caused by the hydrophilic properties and fluidity potential of ethanol in water, which results in dehydration of several cell regions and their structures (Klemm, 1990(Klemm, , 1998. This dehydration produces gross morphological changes in preserved specimens (Berbel-Filho et al, 2013;Bininda-Emonds & Russell, 1994;Buchheister & Wilson, 2005;Cunningham et al, 2000;Deichmann et al, 2009;Gaston et al, 2013;Guillette et al, 1988;Hetherington et al, 2019;Larochelle et al, 2016;Lee, 1982;Moku et al, 2004;Sotola et al, 2019;Thacker & Gkenas, 2019;Vervust et al, 2009;Voight, 2001). For example, Vervust et al (2009) observe that after 68 days of being preserved in ethanol, the average snout-vent length (SVL) of 65 green iguanas (Iguana iguana) is significantly reduced.…”

Section: Known Effects Of Ethanol Preservation Of Specimensmentioning

confidence: 99%

“…Museums commonly use formalin fixation followed by ethanol storage as a means to preserve soft-tissue specimens (Simmons, 2014). Studies have shown that this ethanol storage leads to alterations in the gross morphology of whole specimens (Berbel-Filho et al, 2013;Bininda-Emonds & Russell, 1994;Buchheister & Wilson, 2005;Cunningham, Jr, & Pope, 2000;Deichmann, Boundy, & Williamson, 2009;Gaston, Jacquemin, & Lauer, 2013;Guillette, Rand, Demarco, & Etheridge, 1988;Hetherington, Kurle, Ohman, & Popp, 2019;Larochelle, Tinoco Pickens, Burns, & Sidlauskas, 2016;Lee, 1982;Moku, Mori, & Watanabe, 2004;Sotola et al, 2019;Thacker & Gkenas, 2019;Vervust, Van Dongen, & Van Damme, 2009;Voight, 2001;Ward & Lieber, 2005). Therefore, it is likely histological and architectural alterations are taking place within the musculature as well.…”

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confidence: 99%

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Effects of long‐term ethanol storage on muscle architecture

Leonard

Worden

Boettcher

et al. 2021

The Anatomical Record

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Muscle excursion and force potential can be estimated from architectural variables, including mass, volume, fascicle length, and density. These have been collected from fresh specimens, preserved specimens, and sometimes mixed samples of both. However, preservation alters the gross morphology of muscles. This study aims to quantify the effects of long-term storage on myological properties across a sample of fresh and ethanol preserved Mus musculus specimens ranging in storage time from 16 to 130 years. Masses, volumes, and densities of biceps femoris, quadriceps femoris, and triceps surae were measured, and histological cross-sections of some specimens were used to evaluate the microscale effects of long-term fluid preservation. For the remainder of the sample, chemically dissected fascicle lengths were measured to evaluate the fixation effects on the linear dimensions of muscle architecture. Relative muscle mass, volume, fascicle length, average fiber area, and density, and percent fiber area were regressed against years stored in ethanol. Muscle size dropped steeply between fresh and stored samples, ultimately decreasing by 62 and 60%, respectively. These losses correlate with histologically measured shrinking of average muscle fiber area. Density of stored specimens plateaued 5% below that of fresh ones. Although muscles lost mass and volume during ethanol storage, fascicle lengths did not shorten significantly (presumably because they were preserved attached on either end to bone). This study demonstrates that muscle mass, volume, and density of specimens stored long-term in ethanol should be corrected by factors of 2.64, 2.49, and 1.054 respectively for comparability to fresh specimens.

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Section: Discussionmentioning

confidence: 99%

Section: Known Effects Of Ethanol Preservation Of Specimensmentioning

confidence: 99%

mentioning

confidence: 99%

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Effects of long‐term ethanol storage on muscle architecture

Leonard

Worden

Boettcher

et al. 2021

The Anatomical Record

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Histology and functional morphology of the oviduct of an oviparous snake,Diadophis punctatus

Perkins

Palmer

1996

J. Morphol.

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Morphological changes in the oviductal endometrium during the reproductive cycle of the tortoise,Gopherus polyphemus

Palmer

Guillette

1990

Journal of Morphology

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The oviducts of 24 tortoises (Gopherus polyphemus) were examined using histological techniques and scanning electron microscopy to determine endometrial morphology. Measurements of endometrial characteristics (epithelial cell height, cilia length, thickness of endometrial glandular layer, and glandular diameter) in the uterus and tube (tuba uterina) were obtained to determine changes during the reproductive cycle. Epithelial cell height increases in both the uterus and the tube during vitellogenesis and remains hypertrophied during gravidity. Cilia length increases in the uterus during late vitellogenesis and gravidity, but the length of tubal cilia does not change during the reproductive cycle. The ratio of secretory to ciliated epithelial cells in the oviduct increases from quiescence to gravidity. The thickness of the glandular endometrial layer increases in both the uterus and tube during vitellogenesis. In the uterus, the glandular layer decreases in thickness during gravidity. The diameter of the uterine glands increases throughout vitellogenesis and gravidity; however, following ovulation glandular cells become depleted of secretory granules and cell height diminishes. The diameter of the tubal glands is unchanged during the reproductive cycle. Oviductal hypertrophy during vitellogenesis coincides with elevated circulating estradiol, whereas during gravidity progesterone concentrations peak (Taylor, '82, PhD Dissertation, University of Florida, Gainesville) and may induce secretion of albumen and eggshell components.

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Fixative and Alcohol-Induced Weight Change in Eggs from the Lizard, Sceloporus undulatus

Cited by 5 publications

References 0 publications

Effects of long‐term ethanol storage on muscle architecture

Effects of long‐term ethanol storage on muscle architecture

Histology and functional morphology of the oviduct of an oviparous snake,Diadophis punctatus

Morphological changes in the oviductal endometrium during the reproductive cycle of the tortoise,Gopherus polyphemus

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