Flagella-Driven Chemotaxis Towards Exudate Components Is an Important Trait for Tomato Root Colonization by <i>Pseudomonas fluorescens</i>

Weert, Sandra de; Vermeiren, Hans; Mulders, Ine H. M.; Kuiper, Irene; Hendrickx, Nico W.; Bloemberg, Guido V.; Vanderleyden, Jos; Mot, René De; Lugtenberg, Ben

doi:10.1094/mpmi.2002.15.11.1173

Cited by 513 publications

(348 citation statements)

References 38 publications

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“…1). Again, this finding could reflect differences in levels of key attractants in the two exudates that could conceivably play a role in host selection because chemotaxis plays an important role in successful colonization of the rhizosphere (21,(24)(25)(26)(27)(28). Some genes encoding proteins with functions previously implicated in microbial-plant interactions were down-regulated in response to both root exudates (Fig.…”

Section: Resultsmentioning

confidence: 93%

Transcriptome profiling of bacterial responses to root exudates identifies genes involved in microbe-plant interactions

Mark

Dow

Kiely

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

226

166

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Molecules exuded by plant roots are thought to act as signals to influence the ability of microbial strains to colonize the roots and to survive in the rhizosphere. Differential bacterial responses to signals from different plant species may mediate the selection of specific rhizosphere populations. Very little, however, is known about the effects of plant exudates on patterns of bacterial gene expression. Here, we have tested the concept that plant root exudates modulate expression of bacterial genes involved in establishing microbe-plant interactions. We have examined the influence on the Pseudomonas aeruginosa PA01 transcriptome of exudates from two varieties of sugarbeet that select for genetically distinct pseudomonad populations in the rhizosphere. The response to the two exudates showed only a partial overlap; the majority of those genes with altered expression was regulated in response to only one of the two exudates. Genes with altered expression included those with functions previously implicated in microbe-plant interactions, such as aspects of metabolism, chemotaxis and type III secretion, and a subset with putative or unknown function. Use of a panel of mutants with targeted disruptions allowed us to identify previously uncharacterized genes with roles in the competitive ability of P. aeruginosa in the rhizosphere within this subset. No genes with host-specific effects were identified. Homologues of the genes identified occur in the genomes of both beneficial and pathogenic root-associated bacteria, suggesting that this strategy may help to elucidate molecular interactions that are important for biocontrol, plant growth promotion, and plant pathogenesis.Pseudomonas ͉ Rhizosphere colonization

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Section: Resultsmentioning

confidence: 93%

Transcriptome profiling of bacterial responses to root exudates identifies genes involved in microbe-plant interactions

Mark

Dow

Kiely

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

226

166

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“…Interestingly, we see that the two-component system, chemotaxis family, response regulator involved in bacterial chemotaxis is significantly expressed late in plant development and its expression positively correlates with the root exudates glycine and xylitol (Supplementary Tables S3 and S4). Interestingly, PGPRs and endophytic bacteria show chemotaxis towards glycine (Gaworzewska and Carlile, 1982;de Weert et al, 2002;Gupta Sood, 2003), while the non-symbiotic nitrogen-fixing bacteria Azotobacter vinelandii shows chemotactic behavior towards xylitol (Haneline et al, 1991). This provides an additional mechanism for the plants ability to manipulate and orchestrate the rhizosphere microbiome.…”

Section: Discussionmentioning

confidence: 99%

“…Evidence demonstrating the close ties root exudates have on the microbial composition of the rhizosphere is mounting (Broeckling et al, 2008;Badri et al, , 2013aMicallef et al, 2009b;Chaparro et al, 2012Chaparro et al, , 2013, whereby many chemicals present in root exudates act as substrates, chemotactic or signaling molecules to orchestrate changes in microbial composition (Shaw, 1991;de Weert et al, 2002;Jain and Nainawatee, 2002;Horiuchi et al, 2005;Bais et al, 2006;Badri and Vivanco, 2009;Neal et al, 2012;Badri et al, 2013a). Recently, it was reported that the composition of Arabidopsis root exudates change following a plant developmental gradient (Chaparro et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Rhizosphere microbiome assemblage is affected by plant development

2013

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There is a concerted understanding of the ability of root exudates to influence the structure of rhizosphere microbial communities. However, our knowledge of the connection between plant development, root exudation and microbiome assemblage is limited. Here, we analyzed the structure of the rhizospheric bacterial community associated with Arabidopsis at four time points corresponding to distinct stages of plant development: seedling, vegetative, bolting and flowering. Overall, there were no significant differences in bacterial community structure, but we observed that the microbial community at the seedling stage was distinct from the other developmental time points. At a closer level, phylum such as Acidobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria and specific genera within those phyla followed distinct patterns associated with plant development and root exudation. These results suggested that the plant can select a subset of microbes at different stages of development, presumably for specific functions. Accordingly, metatranscriptomics analysis of the rhizosphere microbiome revealed that 81 unique transcripts were significantly (Po0.05) expressed at different stages of plant development. For instance, genes involved in streptomycin synthesis were significantly induced at bolting and flowering stages, presumably for disease suppression. We surmise that plants secrete blends of compounds and specific phytochemicals in the root exudates that are differentially produced at distinct stages of development to help orchestrate rhizosphere microbiome assemblage.

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“…de Weert et al (2002) established that motility in Pseudomonas is a major trait for root colonization, and the organic acids such as malic acid and citric acid are among major chemo-attractants for P. fluorescens in the tomato rhizosphere. This study is supported by Choi et al (2006) that biofilm formation and swarming motility may contribute to bacterial colonization and stable maintenance of antifungal compounds on plant surface.…”

Section: Resultsmentioning

confidence: 99%

Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping

Subramanian

Satyan

2014

Chilean J. Agric. Res.

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Fluorescent pseudomonads, acclaimed plant associated bacterial group, are well-known plant growth promoting-biocontrol agents in rhizosphere arena. In this study, 144 fluorescent pseudomonad isolates from rhizosphere soil samples were screened with King's medium B supplemented with 8-hydroxyquinoline (8-HQ) chelator and comprehensively profiled for plant growth promotion viz., production of indole acetic acid (IAA), siderophore, ammonia, hydrogen cyanide, motility, phosphate solubilization, root growth promotion, and biofilm forming ability, along with two known control strains of pseudomonads. Iron and IAA regulated secondary metabolite siderophore production were investigated quantitatively. All isolates were positive for ammonia production and motility; 46% isolates were positive for hydrogen cyanide, 44% shown positivity for phosphate solubilization, and 40% isolates for siderophore production. Siderotyping showed production of hydroxamate type of siderophores which are known to be more efficient biocontrol agents. All isolates stimulated root growth to varying extent and had potentiality to form biofilms, a critical constituent for survival on different environments. Forty-two isolates of pseudomonads showed antagonistic behavior against the deleterious fungal pathogen Fusarium oxysporum (MTCC1755). Based on the above observations and statistical analysis, 11 isolates were shortlisted for further scrutiny. The study of biogeographic correlation and secondary metabolite profiling in association with plant growth promotion focalizes significant assessment on the behavior and antagonistic action, which probably brings out a competent biocontrol agent in a sustainable eco-friendly dimension.

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Flagella-Driven Chemotaxis Towards Exudate Components Is an Important Trait for Tomato Root Colonization by Pseudomonas fluorescens

Cited by 513 publications

References 38 publications

Transcriptome profiling of bacterial responses to root exudates identifies genes involved in microbe-plant interactions

Transcriptome profiling of bacterial responses to root exudates identifies genes involved in microbe-plant interactions

Rhizosphere microbiome assemblage is affected by plant development

Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping

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