Flagellin Glycoproteomics of the Periodontitis Associated Pathogen Selenomonas sputigena Reveals Previously Not Described O-glycans and Rhamnose Fragment Rearrangement Occurring on the Glycopeptides

Rath, Cornelia B.; Schirmeister, Falko; Figl, Rudolf; Seeberger, Peter H.; Schäffer, Christina; Kolarich, Daniel

doi:10.1074/mcp.ra117.000394

Cited by 20 publications

(17 citation statements)

References 70 publications

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“…The observed ion at m/z 129.0547 and the calculated m/z value of 129.0546 for C 6 H 9 O 3 + with the mass difference of 0.26 ppm (Figure 4) readily confirmed the structure of an oxonium ion of rhamnose. Normally, oxonium ions of sugar are observed in MS/MS spectra of glycosides [33] and they are useful ions for sugar identification in modern glycoproteomic research [34,35]. To the best of our knowledge, this is the first report on the oxonium ion of rhamnose, C 6 H 9 O 3 + at m/z ca 129.05 and it is possibly used as a characteristic fragment ion for rhamnose in mass spectrometry.…”

Section: Dereplication Of Compounds From Crude Extracts Of V Denticumentioning

confidence: 90%

UHPLC-ESI-QTOF-MS/MS-Based Molecular Networking Guided Isolation and Dereplication of Antibacterial and Antifungal Constituents of Ventilago denticulata

et al. 2020

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Ventilago denticulata is an herbal medicine for the treatment of wound infection; therefore this plant may rich in antibacterial agents. UHPLC-ESI-QTOF-MS/MS-Based molecular networking guided isolation and dereplication led to the identification of antibacterial and antifungal agents in V. denticulata. Nine antimicrobial agents in V. denticulata were isolated and characterized; they are divided into four groups including (I) flavonoid glycosides, rhamnazin 3-rhamninoside (7), catharticin or rhamnocitrin 3-rhamninoside (8), xanthorhamnin B or rhamnetin 3-rhamninoside (9), kaempferol 3-rhamninoside (10) and flavovilloside or quercetin 3-rhamninoside (11), (II) benzisochromanquinone, ventilatones B (12) and A (15), (III) a naphthopyrone ventilatone C (16) and (IV) a triterpene lupeol (13). Among the isolated compounds, ventilatone C (16) was a new compound. Moreover, kaempferol, chrysoeriol, isopimpinellin, rhamnetin, luteolin, emodin, rhamnocitrin, ventilagodenin A, rhamnazin and mukurozidiol, were tentatively identified as antimicrobial compounds in extracts of V. denticulata by a dereplication method. MS fragmentation of rhamnose-containing compounds gave an oxonium ion, C6H9O3+ at m/z 129, while that of galactose-containing glycosides provided the fragment ion at m/z 163 of C6H11O5+. These fragment ions may be used to confirm the presence of rhamnose or galactose in mass spectrometry-based analysis of natural glycosides or oligosaccharide attached to biomolecules, that is, glycoproteins.

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Section: Dereplication Of Compounds From Crude Extracts Of V Denticumentioning

confidence: 90%

UHPLC-ESI-QTOF-MS/MS-Based Molecular Networking Guided Isolation and Dereplication of Antibacterial and Antifungal Constituents of Ventilago denticulata

et al. 2020

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“…Shetty et al reported that Megasphaera has several mechanisms for protection against oxidative stress and harbor multidrug resistance efflux pumps and genes that confer resistance to specific antibiotics, which might account for the growth of Megasphaera [51]. Selenomonas species are colonizers of the digestive system where they act at the interface between health and disease [52]. In the current study, the genus Selenomonas was significantly more abundant in the Lp group compared with the other four groups.…”

Section: Discussionmentioning

confidence: 52%

The Facilitating Effect of Tartary Buckwheat Flavonoids and Lactobacillus plantarum on the Growth Performance, Nutrient Digestibility, Antioxidant Capacity, and Fecal Microbiota of Weaned Piglets

Cui

Wei

Wang

et al. 2019

Animals

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Simple SummaryThere has been a growing interest in the use of flavonoids and probiotics as alternatives of antibiotics in livestock production and as natural products for human health benefit. The effect of tartary buckwheat flavonoid supplementation in the diet has not been clearly investigated. The supplementation of tartary buckwheat flavonoids or Lactobacillus plantarum improved the growth performance, nutrient digestibility, and antioxidant capacity of weaned piglets. In combination, they exhibited a synergistic effect on nutrient digestibility.AbstractNatural plant extracts and probiotics has been proved as the most preferred and effective alternatives for antibiotics in animal feeding. The current study aimed to investigate the facilitating effect of tartary buckwheat flavonoids and Lactobacillus plantarum on the growth performance, nutrient digestibility, antioxidant capacity, and microbiota of weaned piglets. Fifty 35-day-old weaned piglets (7.85 ± 0.67 kg) were randomly divided into five treatments with 10 piglets per treatment. Piglets in the negative control (NC) group were fed a basal diet, and others were fed basal diets supplemented with 40 mg/kg of colistin sulfate (positive control, PC), 109 CFU/kg Lactobacillus plantarum (LP), 40 mg/kg of tartary buckwheat flavonoids (BF), and a combination of 109 CFU/kg Lactobacillus plantarum and 40 mg/kg of tartary buckwheat flavonoids (LB). Supplementation of BF increased the average daily gain of piglets in the BF group (p < 0.05). The nutrient digestibility of piglets in the NC group was lower than that in other groups, while the digestibility of gross energy, dry matter, organic matter, and phosphorus of piglets in the LB group was higher than the other four groups (p < 0.05). Compared with the NC and pC group, supplementation of Lp increased the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-px), and catalase (CAT), while the BF increased the content of IgA and IgM (p < 0.05). Supplementation of colistin sulfate decreased the alpha diversity index, including chao and observed species, while the addition of Lp or combination of Lp and BF increased the abundance of Selenomonas or Mitsuokella in fecal samples, respectively. The results indicated that supplementation of Lactobacillus plantarum can improve the antioxidant capacity, while tartary buckwheat flavones can increase the growth performance and immune ability of weaned piglets. Moreover, in combination, they promote nutrient digestibility.

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“…Furthermore, in a seminal study, Kolenbrander and colleagues (Kolenbrander et al, 1989 ) demonstrated that Selenomonas organisms ( S. sputigena, S. flueggei, S. infelix , and S. noxia ) co-aggregated with Fusobacterium nucleatum through cell-to-cell interactions; except for S.infelix , we found these species in the sputum of TB patients. Recently, it has been shown that S. sputigena has a heavily glycosylated flagella (Rath et al, 2018 ) which likely contributes to the capability of Selenomonas spp . to form multispecies aggregates (biofilms) with members of other genera in dental plaque.…”

Section: Discussionmentioning

confidence: 99%

The Sputum Microbiome in Pulmonary Tuberculosis and Its Association With Disease Manifestations: A Cross-Sectional Study

et al. 2021

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Each day, approximately 27,000 people become ill with tuberculosis (TB), and 4,000 die from this disease. Pulmonary TB is the main clinical form of TB, and affects the lungs with a considerably heterogeneous manifestation among patients. Immunomodulation by an interplay of host-, environment-, and pathogen-associated factors partially explains such heterogeneity. Microbial communities residing in the host's airways have immunomodulatory effects, but it is unclear if the inter-individual variability of these microbial communities is associated with the heterogeneity of pulmonary TB. Here, we investigated this possibility by characterizing the microbial composition in the sputum of 334 TB patients from Tanzania, and by assessing its association with three aspects of disease manifestations: sputum mycobacterial load, severe clinical findings, and chest x-ray (CXR) findings. Compositional data analysis of taxonomic profiles based on 16S-rRNA gene amplicon sequencing and on whole metagenome shotgun sequencing, and graph-based inference of microbial associations revealed that the airway microbiome of TB patients was shaped by inverse relationships between Streptococcus and two anaerobes: Selenomonas and Fusobacterium. Specifically, the strength of these microbial associations was negatively correlated with Faith's phylogenetic diversity (PD) and with the accumulation of transient genera. Furthermore, low body mass index (BMI) determined the association between abnormal CXRs and community diversity and composition. These associations were mediated by increased abundance of Selenomonas and Fusobacterium, relative to the abundance of Streptococcus, in underweight patients with lung parenchymal infiltrates and in comparison to those with normal chest x-rays. And last, the detection of herpesviruses and anelloviruses in sputum microbial assemblage was linked to co-infection with HIV. Given the anaerobic metabolism of Selenomonas and Fusobacterium, and the hypoxic environment of lung infiltrates, our results suggest that in underweight TB patients, lung tissue remodeling toward anaerobic conditions favors the growth of Selenomonas and Fusobacterium at the expense of Streptococcus. These new insights into the interplay among particular members of the airway microbiome, BMI, and lung parenchymal lesions in TB patients, add a new dimension to the long-known association between low BMI and pulmonary TB. Our results also drive attention to the airways virome in the context of HIV-TB coinfection.

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Flagellin Glycoproteomics of the Periodontitis Associated Pathogen Selenomonas sputigena Reveals Previously Not Described O-glycans and Rhamnose Fragment Rearrangement Occurring on the Glycopeptides

Cited by 20 publications

References 70 publications

UHPLC-ESI-QTOF-MS/MS-Based Molecular Networking Guided Isolation and Dereplication of Antibacterial and Antifungal Constituents of Ventilago denticulata

UHPLC-ESI-QTOF-MS/MS-Based Molecular Networking Guided Isolation and Dereplication of Antibacterial and Antifungal Constituents of Ventilago denticulata

The Facilitating Effect of Tartary Buckwheat Flavonoids and Lactobacillus plantarum on the Growth Performance, Nutrient Digestibility, Antioxidant Capacity, and Fecal Microbiota of Weaned Piglets

The Sputum Microbiome in Pulmonary Tuberculosis and Its Association With Disease Manifestations: A Cross-Sectional Study

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