Flagellin Redundancy in Caulobacter crescentus and Its Implications for Flagellar Filament Assembly

Faulds-Pain, Alexandra; Birchall, Christopher; Aldridge, Christine; Smith, William Thomas Lingwood; Grimaldi, Giulia; Nakamura, Shuichi; Miyata, Tomoko; Gray, Joe; Li, Guanglai; Tang, Jay X.; Namba, Keiichi; Minamino, Tetsuo; Aldridge, Phillip D.

doi:10.1128/jb.01172-10

Cited by 56 publications

(81 citation statements)

References 48 publications

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“…Intriguingly, only MO-1 and the closely related magnetotactic coccus MC-1 contain more than seven flagellin genes. This result is consistent with a similar observation recently reported by FauldsPain et al 16 We then addressed the question whether the 14 flagellin or putative flagellin genes were expressed. Their expression was examined by reverse transcription PCR (RT-PCR).…”

Section: Mo-1 Genome Contains 14 Flagellin Genessupporting

confidence: 92%

Complex Spatial Organization and Flagellin Composition of Flagellar Propeller from Marine Magnetotactic Ovoid Strain MO-1

Zhang

Santini

Bernadac

et al. 2012

Journal of Molecular Biology

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Section: Mo-1 Genome Contains 14 Flagellin Genessupporting

confidence: 92%

Complex Spatial Organization and Flagellin Composition of Flagellar Propeller from Marine Magnetotactic Ovoid Strain MO-1

Zhang

Santini

Bernadac

et al. 2012

Journal of Molecular Biology

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“…Thanks to the availability of a large number of bacterial genome sequences, we now know profound variations in flagellins from three aspects: (i) widespread (ϳ45% of bacteria host multiple copies of flagellins); (ii) gene copies (the maximum number of flagellin genes in one single genome is 15); and (iii) flagellin size (whereas the largest is 670 aa in length, 250 aa is the minimum required for functionality) (3,30). Given the high level of sequence similarity among multiple flagellins in any bacterial species, these subunits are undoubtedly originated from gene duplications (1).…”

Section: Discussionmentioning

confidence: 99%

Two Residues Predominantly Dictate Functional Difference in Motility between Shewanella oneidensis Flagellins FlaA and FlaB

Sun

Dong

Shi

et al. 2014

Journal of Biological Chemistry

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Background:Flagellins with little sequence variability in multiple-flagellin systems nonetheless differ substantially in function. Results: Motility differences resulting from S. oneidensis flagellin paralogs FlaA and FlaB are due to amino acid composition rather than regulation. Conclusion: Two residue positions predominantly dictate differences in motility mediated by the two flagellins. Significance: This work provides great insight into our understanding of functional difference between duplicated proteins.

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“…These data support our idea that the ability of bacteria to swim in structured environments is driven by flagellar polymorphism. However, Caulbacter cresentus and V. alginolyticus , form a swarm ring on a semi-solid agar plate without flagellar polymorphism [55, 57]. In these bacteria, the hydrodynamic load causes the buckling of the straight hook, upon the motor switching from CW to CCW rotation [8, 9, 58].…”

Section: Discussionmentioning

confidence: 99%

Distinct chemotactic behavior in the originalEscherichia coliK-12 depending on forward-and-backward swimming, not on run-tumble movements

Kinosita

Ishida

Yoshida

et al. 2020

Preprint

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Most motile bacteria are propelled by rigid, helical, flagellar filaments and display distinct swimming patterns to explore their favorable environments. Escherichia coli cells have a reversible rotary motor at the base of each filament. They exhibit a run-tumble swimming pattern, driven by switching of rotatory direction which causes polymorphic flagellar transformation. Here we report a novel swimming mode in E. coli ATCC10798, which is one of the original K-12 clones. High-speed tracking of single ATCC10798 cells showed forward and backward swimming with an average turning angle of 150°. The flagellar helicity remained right-handed with a 1.3 μm pitch and 0.14 μm helix radius, which is assumed to be a curly type, regardless of motor switching; the flagella of ATCC10798 did not show polymorphic transformation. The torque and rotational switching of the motor was almost identical to the E. coli W3110 strain, which is a derivative of K-12 and a wild-type for chemotaxis. The single point mutation of N87K in FliC, one of the filament subunits, is critical to the change in flagellar morphology and swimming pattern, and lack of flagellar polymorphism. E. coli cells expressing FliC(N87K) sensed ascending a chemotactic gradient in liquid but did not form rings on a semi-solid surface. Based on these findings, we propose a flagellar polymorphism-dependent migration mechanism in structured environments.

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Flagellin Redundancy in Caulobacter crescentus and Its Implications for Flagellar Filament Assembly

Cited by 56 publications

References 48 publications

Complex Spatial Organization and Flagellin Composition of Flagellar Propeller from Marine Magnetotactic Ovoid Strain MO-1

Complex Spatial Organization and Flagellin Composition of Flagellar Propeller from Marine Magnetotactic Ovoid Strain MO-1

Two Residues Predominantly Dictate Functional Difference in Motility between Shewanella oneidensis Flagellins FlaA and FlaB

Distinct chemotactic behavior in the originalEscherichia coliK-12 depending on forward-and-backward swimming, not on run-tumble movements

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