This paper describes simulation of combustion characteristics under high ambient and high injection of biodiesel combustion by using CFD simulation. Diesel engine performance and emissions is strongly couple with fuel atomization and spray processes, which in turn are strongly influenced by injector flow dynamics. The principal objective of this research is to seek the effect of temperature and pressure on the spray characteristics, as well as fuel-air mixing characteristics. Experiments were performed in a constant volume chamber at specified ambient gas temperature and pressure. This research was continued with injecting diesel fuel into the chamber using a Bosch common rail system. Direct photography technique with a digital camera was used to clarify the real images of spray pattern, liquid length and vapor penetration. The method of the simulation of real phenomenon of diesel combustion with optical access rapid compression machine is also reviewed and experimental results are presented. The liquid phase of the spray reaches a maximum penetration distance soon after the start of injection, while the vapor phase of the spray continues to penetrate downstream. The condition to which the fuel is affected was estimated by combining information on the block temperature, ambient temperature and photographs of the spray. The increases in ambient pressure inside the chamber resulting in gain of spray area and wider spray angle. Thus predominantly promotes for a better fuel-air mixing. All of the experiments will be conducted and run by using CFD. The simulation will show in the form of images.