Flame photometric detector for thin-layer chromatography

Ogasawara, Minoru; Tsuruta, Kyoko; Arao, Shinsuke

doi:10.1016/s0021-9673(02)01117-2

Cited by 12 publications

(7 citation statements)

References 28 publications

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“…The distribution of each subcellular fraction was judged by standard enzymatic measurements, cytochrome c oxidase (mitochondrial marker), NADPH‐cytochrome c reductase (microsomal marker), and lactate dehydrogenase (cytosolic marker) as reported previously 11. The cell constituents were extracted with chloroform and methanol (2:1 by volume), and lipids in the chloroform layer were analyzed by using a thin‐layer chromatography equipped with a flame ionization detection system 12. The total molar lipid content of each cell fraction was determined using these authentic samples and average molecular weight as follows: for phosphatidylcholine, 1,2‐dimyristoyl‐rac‐glycero‐3‐phosphocholine (Sigma, St. Louis, MO) and 677.9; for phosphatidylethanolamine, l‐α‐phosphatidylethanolamine from egg yolk (Sigma) and 746.1; for triacylglycerol, triolein (Nacalai, Kyoto, Japan) and 885.4; for cholesterol, cholesterol (Sigma) and 386.7.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Cellular Uptake and Distribution of Vitamin E

Saito

Yoshida

Nishio

et al. 2004

Annals of the New York Academy of Sciences

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We previously reported that tocotrienols acted as more potent inhibitors against selenium deficiency-induced cell death than the corresponding tocopherol isoforms (J. Biol. Chem. 2003;278:39428-39434). In the present study, we first compared the differences in the cellular uptake between alpha-tocopherol (alpha-Toc) and alpha-tocotrienol (alpha-Toc-3). The initial rate of cellular uptake of alpha-Toc-3 was 70-fold higher than that of alpha-Toc. Subcellular fractionation analysis of alpha-Toc-3 and alpha-Toc-fortified cells showed similar cellular distribution of these antioxidants, which was directly proportional to the lipid distribution. The cells containing similar amounts of alpha-Toc-3 and alpha-Toc showed similar resistance against the oxidative stress caused by peroxides. These results suggest that the apparent higher cytoprotective effect of alpha-Toc-3 than alpha-Toc is primarily ascribed to its higher cellular uptake.

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Section: Methodsmentioning

confidence: 99%

Characterization of Cellular Uptake and Distribution of Vitamin E

Saito

Yoshida

Nishio

et al. 2004

Annals of the New York Academy of Sciences

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“…The cell constituents were extracted with chloroform and methanol (1:5:10, v/v), and lipids in the chloroform layer were analyzed by using a thin-layer chromatography equipped with a flame ionization detection system [24]. The total molar lipid content of each cell fraction was determined using these authentic samples and average molecular weight as follows: for phosphatidylcholine, 1,2-dimyristoyl-rac-glycero-3-phosphocholine (Sigma) and 677.9; for phosphatidylethanolamine, L-α-phosphatidylethanolamine from egg yolk (Sigma) and 746.1; for triacylglycerol, triolein (Nacalai) and 885.4; for cholesterol, cholesterol (Sigma) and 386.7.…”

Section: Determination Of Intracellular Lipidsmentioning

confidence: 99%

Characterization of cellular uptake and distribution of coenzyme Q10 and vitamin E in PC12 cells☆

Saito

Fukuhara

Nishio

et al. 2009

The Journal of Nutritional Biochemistry

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“…The equipment typically used in the bituminous materials industry to perform the TLC is IATROSCAN (Ogasawa et al 2002). It can be equipped in the flame ionization detector (FID) or with Flame Photometric Detector (FPD).…”

Section: Introductionmentioning

confidence: 99%

“…It can be equipped in the flame ionization detector (FID) or with Flame Photometric Detector (FPD). The FPD is in principle an FID, extended by a filter and a photomultiplier tube (Ogasawa et al 2002). Due to the increasing interest in sulfur (S) distribution in bituminous fractions and their effect on bitumen properties (Makowska et al 2017;McKenna et al 2013) the FPD with a filter at 394 nm wavelength is explored (S-FPD).…”

Section: Introductionmentioning

confidence: 99%

Qualitative Detection of the Presence of Gilsonite in the Bituminous Blends Based on Thin Layer Chromatography

Makowska

Pellinen

2018

RILEM Bookseries

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The natural asphalts, of which Gilsonite is a representative, are modifiers used to increase the stiffness modulus of the asphalt concrete. For quality control purpose, recognizing if such material is present in the final blend is of an interest. The thin layer chromatography using a flame photometric detector (FPD), in addition to the typical flame ionization detector, was demonstrated hereby as a viable analytical tool for this problem. Gilsonite also contains the material soluble in solvent used in the development of the fraction referred to as aromatics. However, for straight run bitumen and Gilsonite the color of that fraction is different as well as their mobility. Due to the lower mobility of Gilsonite molecules on the stationary phase, the elution stops at different position than for fresh bitumen, convoluting the signal in the chromatogram region typically assigned for resins. The presence of Gilsonite is identifiable visually after the second development bath, but also with FPD from the final chromatogram.

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Flame photometric detector for thin-layer chromatography

Cited by 12 publications

References 28 publications

Characterization of Cellular Uptake and Distribution of Vitamin E

Characterization of Cellular Uptake and Distribution of Vitamin E

Characterization of cellular uptake and distribution of coenzyme Q10 and vitamin E in PC12 cells☆

Qualitative Detection of the Presence of Gilsonite in the Bituminous Blends Based on Thin Layer Chromatography

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