Flanking SSR markers for alleles involved in the necrosis of hybrids between hexaploid bread wheat and synthetic hexaploid wheat

Kandel, Devi R.; Glover, Karl D.; Berzonsky, William A.; Gonzalez-Hernandez, Jose L.; Ali, Shahjahan; Chao, Shiaoman; Bhusal, Siddhi J.

doi:10.1080/15427528.2017.1365794

Cited by 1 publication

(3 citation statements)

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“…Previously, Ne1 was considered as a dominant gene based on the segregation ratio of necrotic phenotype in populations derived from the cross between two parents carrying Ne1Ne1ne2ne2 and ne1ne1Ne2Ne2 (Bizimungu et al 1998;Hermsen 1963a;Kandel et al 2017). The Ne1 had the dosage effect and carrier of Ne1Ne1Ne2Ne2 showed heavier necrotic symptoms, compared to Ne1ne1Ne2Ne2 (Hermsen 1963b).…”

Section: Discussionmentioning

confidence: 99%

“…Hybrid necrosis is controlled by the interaction of two complementary dominant genes Ne1 and Ne2, located in chromosome arms 5BL and 2BS, respectively (Chu et al 2006; Kandel et al 2017;Nishikawa et al 1974;Pukhalskiy et al 2018;Zeven 1972). Both Ne1 and Ne2 genes are widely distributed among tetraploid and hexaploid wheat (Takumi et al 2013;Tsunewaki 1992;Vikas et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Chu et al (2006) mapped the hybrid necrosis genes Ne1 and Ne2 using SSR markers and demonstrated that markers Xbarc74-5B and Xbarc55-2B were closely linked to Ne1 and Ne2 at genetic distances of 2.0 cM and 3.2 cM, respectively. Due to the genetic distances between the markers and Ne genes and the lack of coordination of alleles of markers with alleles of Ne genes, these two markers were only adopted by several studies in assessing the Ne genes in wheat cultivars (Galaiev 2016;Kandel et al 2017). Therefore, it is necessary to develop a more convenient and e cient diagnostic marker for assessing the Ne genes in wheat germplasm.…”

Section: Introductionmentioning

confidence: 99%

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Fine mapping of hybrid necrosis gene Ne1 in common wheat (Triticum aestivum L.)

Zheng

Niu

et al. 2021

Theor Appl Genet

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Hybrid necrosis is characterized as progressive chlorosis and necrosis of plant leaves, tillers or whole plants premature death in certain wheat hybrids, which is caused by the combination of two complementary loci Ne1 and Ne2 located on chromosome arms 5BL and 2BS, respectively. Hybrid necrosis s is a serious barrier for combining desirable traits from various genotypes of wheat and the full utilization of heterosis in wheat. In this study, we ne mapped Ne1 on chromosome 5BL, and nally delimited Ne1 to a 4.06-Mb region with large heterogeneous inbred families (HIFs) from the RILs of 'Zhengnong 17' × 'Yangbaimai'. Further characterization of the near isogenic lines derived from HIFs con rmed that Ne1 was tightly linked with a 2.89-Mb fragment that is deleted in Zhengnong 17. Additionally, a diagnostic InDel marker 5B-InDel385 for Ne1 was developed and was used to assess the existence of Ne1 in a diverse panel of 501 wheat accessions. Among them, 122 (61%) out of 200 landraces showed the presence of Ne1 gene, whereas only 79 (26%) out of 301 modern cultivars carried the Ne1 gene. The drastic decrease of Ne1 frequency in modern cultivars indicates that the Ne1 has been subjected to heavily selection pressure. This study provides a good foundation for the marker-assisted selection, gene cloning and functional studies of Ne1 in wheat. Key MessageHybrid necrosis gene Ne1 was delimited into an approximately 4.06-Mb region on chromosome 5BL using heterogeneous inbred families, and a diagnostic marker for Ne1 was developed.

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Section: Discussionmentioning

confidence: 99%