Flavanols and Flavanes from Crinum asiaticum and Their Effects on LPS Signaling Pathway Through the Inhibition of NF-κB Activation

Abstract

Three new flavanols, (2R,3S)-7-methoxy-flavan-3-ol (1), (2R,3S)-7-hydroxy-flavan-3-ol (2), and (2R,3S)-2′-hydroxy-7-methoxy-flavan-3-ol (3), together with two known flavans (4 and 5), were isolated from the chloroform extract of Crinum asiaticum. Their structures were elucidated by various spectroscopic methods, including 1D and 2D NMR, HR-ESI-MS, and CD data. The isolated compounds 1 and 3−5 showed inhibitory activity toward LPS-induced nitric oxide (NO) production. Further investigation of the NF-κB pathway mechanisms indicated that 1 and 3−5 inhibited the LPS-induced IL-6 production and p65 subunit phosphorylation of NF-κB in RAW264.7 cells, with an effective dose of 10 µM.

Effects of Sophora alopecuroides in a High-Concentrate Diet on the Liver Immunity and Antioxidant Function of Lambs According to Transcriptome Analysis

“…A portion of free LPS enters the bloodstream from the gastrointestinal tract and then enters the liver through the portal vein, whereas uncleared LPS in the liver induces structural and functional changes in the liver [28] and stimulates the secretion of proinflammatory cytokines by hepatocytes and lymphocytes, which in turn activates the inflammatory response [29]. The other part of the LPS complex binds to LBP (LBP is a glycoprotein synthesized in hepatocytes and secreted into the serum) on the cell membrane to form a complex that acts on cell membrane receptors to promote the release of inflammatory mediators such as TNFα, NO and IL-6 through MAPK, NF-κB and other signaling pathways, thereby triggering inflammatory responses in the liver and causing indirect damage to the liver [30,31]. In the present study, the LPS and LBP contents in the blood and liver were markedly higher in the H2 group than in the C1 and S3 groups, indicating that LPS-induced production by high-concentrate diets causes a systemic inflammatory response.…”

The Phytochemistry and Pharmacology of Tulbaghia, Allium, Crinum and Cyrtanthus: ‘Talented’ Taxa from the Amaryllidaceae

“…This activity whi1ch was later attributed to lycorine, an active compound in C. asiaticum [ 297 ]. Some alkaloids isolated from the bulbs of C. asiaticum have been reported to show remarkable inhibition against tumor cell lines A549, LOVO, HL-60, and 6T-CEM [ 261 ].…”