Flavescence Dorée-Derived Leaf Yellowing in Grapevine (Vitis vinifera L.) Is Associated to a General Repression of Isoprenoid Biosynthetic Pathways

Teixeira, António; Martins, Viviana; Frusciante, Sarah; Cruz, T. Sta.; Noronha, Henrique; Diretto, Gianfranco; Gerós, Hernâni

doi:10.3389/fpls.2020.00896

Cited by 11 publications

(19 citation statements)

References 79 publications

(101 reference statements)

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“…Pearson correlations were calculated using MetScape plugin from Cytoscape version 3.8.2 () from a matrix of metabolite and transcript values of grape berries and leaves normalized on green stage values (Supplementary Table 2), as reported by Diretto et al (2010) and Teixeira et al. (2020) …”

Section: Methodsmentioning

confidence: 99%

“…Pearson correlations were calculated using MetScape plugin from Cytoscape version 3.8.2 (www.cytoscape.org) from a matrix of metabolite and transcript values of grape berries and leaves normalized on green stage values (Supplementary Table 2), as reported by Diretto et al (2010) 37 and Teixeira et al (2020). 38 The network diagram was generated with Pearson correlation values (ρ ≥ 0.90 and ρ ≤ −0.90) using a compound profuse force directed layout algorithm. Node strength (ns) calculation, intended as the average of all of the |ρs| yielded by each node, was used to identify the main elements ("hubs") of the network (Supplementary Table 2).…”

Section: Metabolomics Analysismentioning

confidence: 99%

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Biosynthesis of Chlorophyll and Other Isoprenoids in the Plastid of Red Grape Berry Skins

Teixeira

Noronha

Frusciante

et al. 2023

J. Agric. Food Chem.

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Despite current knowledge showing that fruits like tomato and grape berries accumulate different components of the light reactions and Calvin cycle, the role of green tissues in fruits is not yet fully understood. In mature tomato fruits, chlorophylls are degraded and replaced by carotenoids through the conversion of chloroplasts in chromoplasts, while in red grape berries, chloroplasts persist at maturity and chlorophylls are masked by anthocyanins. To study isoprenoid and lipid metabolism in grape skin chloroplasts, metabolites of enriched organelle fractions were analyzed by high-performance liquid chromatography-high-resolution mass spectrometry (HPLC-HRMS) and the expression of key genes was evaluated by real-time polymerase chain reaction (PCR) in berry skins and leaves. Overall, the results indicated that chloroplasts of the grape berry skins, as with leaf chloroplasts, share conserved mechanisms of synthesis (and degradation) of important components of the photosynthetic machinery. Some of these components, such as chlorophylls and their precursors, and catabolites, carotenoids, quinones, and lipids have important roles in grape and wine sensory characteristics.

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Section: Methodsmentioning

confidence: 99%

Section: Metabolomics Analysismentioning

confidence: 99%

Biosynthesis of Chlorophyll and Other Isoprenoids in the Plastid of Red Grape Berry Skins

Teixeira

Noronha

Frusciante

et al. 2023

J. Agric. Food Chem.

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“…Correlations between yeast species were assembled using the MetScape plugin from Cytoscape version 3.8.1 (www. cytoscape.org; Cline et al, 2007), according to their overall response to all tested elicitors, normalised to the respective control without treatment (Teixeira et al, 2020). In the network diagram, generated using a perfuse force direct layout algorithm, node size is proportional to the correlation node strength.…”

Section: Network Analysismentioning

confidence: 99%

“…Positive (ρ > 0.10) and negative (ρ < -0.10) correlations were coloured in red and blue, respectively. MCODE plugin was used to obtain a series of metrical topological parameters of the network, allowing, on a mathematical basis, to unravel the denser areas and distinguish the subnetworks with strong cross-links (Teixeira et al, 2020).…”

Section: Network Analysismentioning

confidence: 99%

A catalogue of cultivable yeasts from the microbiota of grape berries cv. Vinhão and Loureiro

et al. 2022

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The relevance of non-Saccharomyces native yeasts for improving the complexity of wine aroma is increasingly recognised, besides their use in a range of biotechnological applications. Previously, we used a metagenomic approach to assess the effects of vineyard calcium sprays on the microbiota of grape berries cv. Vinhão and Loureiro. In this study, we aimed at assembling a catalogue of the cultivable yeasts from grape berries of these cultivars and to investigate the direct effect of calcium and other elicitors on the isolated taxa. Seventeen unique colony morphologies were identified, and the sequencing of the region spanning the rDNA internal transcribed spacers or the rDNA D1/D2 domain revealed that they are distributed into 13 different genera. Although the cultivar and vintage influenced the taxa abundance and diversity, Aureobasidium pullulans and Hanseniaspora uvarum were the most abundant species, ubiquitously populating the fruits together with Pichia terricola, Rhodotorula glutinis, Starmerella bacillaris and Vishniacozyma heimaeyensis. The rarest genera comprised Candida, Trichosporon and Zygoascus. Analysis of yeast growth in the presence of specific elicitors revealed that Pichia spp. are the most sensitive to exogenous calcium, while Sporobolomyces roseus is the most sensitive to copper exposure. Moreover, A. pullulans, H. uvarum and S. bacillaris displayed the highest sensitivity to osmotic stress. In turn, Wickerhamomyces anomalus, Metschnikowia pulcherrima, P. fermentans, P. kluyveri and P. kudriavzevii showed mild resistance to ethanol, unlike the remaining non-Saccharomyces yeasts. Correlation networks expressing the overall yeast performance showed that H. uvarum and S. bacillaris share the strongest positive interspecies correlation, followed by the pairs P. fermentans–P. kluyveri, B. albus–R. glutinis and A. pullulans–V. heimaeyensis. The strongest negative correlations were found for the pairs P. kluyveri–S. roseus, P. terricola–W. anomalus, P. kudriavzevii–V. heimayensis, P. kluyveri–V. heimayensis, and A. pullulans–P. kluyveri. This study exposed microbial niches with a predictably common response to abiotic stress.

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“…Different studies have reported harmful effects of the infection at both physiological and molecular levels, revealing modifications in the primary and secondary metabolism. This is the case of the isoprenoid biosynthetic pathways, which are repressed in response to infection, accounting for leaf yellowing, which is a hallmark of the disease (Teixeira et al, 2020). Other studies have revealed that the accumulation of sucrose and starch in the leaf mesophyll is accompanied by the up-regulation of key genes involved in their synthesis, including Sucrose synthase 4 (VvSusy4) (Hren et al, 2009;Margaria et al, 2014;Prezelj et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The grapevine metabolite profile of phloem sap is modified by flavescence dorée

et al. 2023

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Flavescence dorée (FD) has been recorded in French vineyards since the mid-1950s; it has rapidly become a major threat to vineyard survival in different European grape-growing areas. Infection by the phytoplasma ‘Candidatus Phytoplasma vitis’ causes leaf chlorosis and disturbs primary and secondary core metabolic pathways; it can also occlude sieve plates, therefore affecting normal solute transport through the phloem. Thus, in the present study we hypothesised that the metabolomic fingerprint of the phloem sap changes in FDp-infected vines. The metabolome of the phloem sap collected by centrifugation from the bark of the stem of healthy and infected vines (cv. ‘Loureiro’) was profiled by ESI-HRMS at two snapshot time points: E-L 33 (grape berry still hard and green) and E-L 38 (grape berry at maturity). An untargeted analysis of the phloem sap enabled the identification of 476 metabolites, whereas the targeted analysis focused on eight main classes, namely acids, sugar and polyols, amino acids, phenolic acids, hormones, vitamins and N compounds, as well as on several minor classes like fatty acids, phenols or diols. Depending on the developmental stage of the vine, major differences were observed in the composition of cv. ‘Loureiro’ phloem sap in response to FD infection in terms of sugar, organic acid and amino acid content; furthermore, compounds involved in plant defense, such as salicylic acid, ABA, vitamin C and gallic acid also significantly changed. Overall, these results have contributed towards increasing knowledge about the complex interactions between vine and FD phytoplasma.

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Flavescence Dorée-Derived Leaf Yellowing in Grapevine (Vitis vinifera L.) Is Associated to a General Repression of Isoprenoid Biosynthetic Pathways

Abstract: Overall, results indicated that the FD infection impairs the synthesis of isoprenoids, through the repression of key genes involved in the biosynthesis of chlorophylls, carotenoids, quinones and tocopherols.

Cited by 11 publications

References 79 publications

Biosynthesis of Chlorophyll and Other Isoprenoids in the Plastid of Red Grape Berry Skins

Biosynthesis of Chlorophyll and Other Isoprenoids in the Plastid of Red Grape Berry Skins

A catalogue of cultivable yeasts from the microbiota of grape berries cv. Vinhão and Loureiro

The grapevine metabolite profile of phloem sap is modified by flavescence dorée

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