Flavobacterium humidisoli sp. nov., isolated from riverside soil

Abstract

A novel Gram-stain-negative, yellow-pigmented, non-motile and rod-shaped bacterial strain designated MMS21-Er5T was isolated and subjected to polyphasic taxonomic characterization. MMS21- Er5T could grow at 4–34 °C (optimum, 30 °C), at pH 6–8 (optimum, pH 7) and in the presence of 0–2% NaCl (optimum, 1 %). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that MMS21- Er5T showed low levels of sequence similarities with other species, as the highest similarity of 97.83 % was observed with Flavobacterium tyrosinilyticum THG DN8.8T, then 97.68 % with ‘Flavobacterium ginsengiterrae’ DCY 55 and 97.63 % with Flavobacterium banpakuense 15F3T, which were well below the suggested cutoff for species distinction. The whole genome sequence of MMS21-Er5T consisted of a single contig of 5.63 Mbp, and the DNA G+C content was 34.06 mol%. The in-silico DNA–DNA hybridization and orthologous average nucleotide identity values were highest with Flavobacterium tyrosinilyticum KCTC 42726T (45.7 and 91.92% respectively). The predominant respiratory quinone for the strain was menaquinone-6 (MK-6), the major cellular fatty acid was iso-C15 : 0, and the diagnostic polar lipids were phosphatidylethanolamine and phosphatidyldiethanolamine. The combination of physiological and biochemical tests clearly distinguished the strain from related species of the genus Flavobacterium . On the basis of these results, strain MMS21-Er5T evidently represents a novel species of the genus Flavobacterium, for which the name Flavobacterium humidisoli sp. nov. is proposed (type strain=MMS21-Er5T=KCTC 92256T =LMG 32524T).

Paraburkholderia translucens sp. nov. and Paraburkholderia sejongensis sp. nov., isolated from grassland soil and showing plant growth promoting potential

“…Extraction of genomic DNA and amplification of 16S rRNA gene were performed as previously described [12], and almost-complete sequences of MMS20-SJTN17 T (1399 bases) and MMS20-SJTR3 T (1433 bases) were obtained. Taxonomic identification was carried out using EzBioCloud [13], and sequence alignment using EzEditor [14], respectively.…”

Description of Microcella humidisoli sp. nov. and Microcella daejeonensis sp. nov., isolated from riverside soil, reclassification of Marinisubtilis pacificus as Microcella pacifica comb. nov., and emended description of the genus Microcella

“…Riverside soil environments harbour a great deal of prokaryotic diversity with various metabolic potential, from which we isolated and described a number of novel taxa in recent years [4][5][6][7][8][9][10]. In this study, a polyphasic taxonomic approach including phylogenetic, phenotypic and chemotaxonomic characterization was employed to describe two new species of Microcella isolated from riverside soil.…”