Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments

A novel moderately thermophilic, red-pigmented bacterium, designated strain AZM34c11 T , was isolated from the bottom of a 1000 m-deep drilled well located in a Japanese hot spring. Cells were Gram-negative and grew optimally at 50 6C, at pH 7.6 and with 0-0.3 % (w/v) NaCl. Analysis of the 16S rRNA gene sequence revealed that the isolate belonged to the genus Meiothermus. Levels of 16S rRNA gene sequence similarity between strain AZM34c11 T and the type strains of recognized Meiothermus species were 88.2-94.8 %. Strain AZM34c11 T was distinguished from recognized Meiothermus species by its cellular fatty acid profile: iso-C 16 : 0 was one of the predominant components and hydroxy fatty acids were present only in trace amounts. The strain could also be differentiated based on its growth substrate preferences and characteristic enzyme reactions. On the basis of these results, strain AZM34c11 T is considered to represent a novel species of the genus Meiothermus, for which the name Meiothermus hypogaeus sp. nov. is proposed. The type strain is AZM34c11 T (5NBRC 106114 T 5DSM 23238 T ).

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“…Spore formation and motility were not observed. Cells were catalasenegative and oxidase-positive (Tamaki et al, 2003).…”

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Meiothermus hypogaeus sp. nov., a moderately thermophilic bacterium isolated from a hot spring

Mori

Iino

Ishibashi

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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“…The physiological characteristics of members of the genus Flavobacterium are also diverse: they can be psychrophilic, psychrotolerant or mesophilic; they can be halotolerant, halophilic or sensitive to salts; and they produce a variety of enzymes (Humphry et al, 2001;Tamaki et al, 2003;Aslam et al, 2005). These findings suggest that the genus Flavobacterium may have important environmental roles.…”

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Flavobacterium croceum sp. nov., isolated from activated sludge

Park

Ryu

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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A Gram-negative, non-motile, rod-shaped bacterium, designated strain EMB47 T , was isolated from activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor. Growth was observed between 10 and 40 6C (optimum, 25-35 6C) and between pH 5?0 and 8?5 (optimum, pH 7?5-8?0). The predominant fatty acids of strain EMB47 T were iso-C 16 : 0 3-OH, iso-C 15 : 1 G, C 15 : 0 , iso-C 15 : 0 , iso-C 14 : 0 and iso-C 16 : 0 and it contained phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylcholine as polar lipids. The G+C content of the genomic DNA was 40?8 mol% and the major quinone was MK-6. Comparative 16S rRNA gene sequence analyses showed that strain EMB47 T formed a distinct phyletic line within the genus Flavobacterium. The levels of 16S rRNA gene sequence similarity with respect to Flavobacterium species were below 94?7 %. On the basis of the phenotypic, chemotaxonomic and molecular data, strain EMB47 T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium croceum sp. nov. is proposed. The type strain is EMB47 T (=KCTC 12611 T =DSM 17960 T ).Since the genus Flavobacterium, belonging to the phylum Bacteroidetes (formerly the Cytophaga-FlavobacteriumBacteroides group), was emended (Bernardet et al., 1996), several novel Flavobacterium species have been described: these have been isolated from diverse habitats such as micromats, fresh water, seawater, Antarctic lakes, soil, the gut of an earthworm and from sediments (McCammon & Bowman, 2000;Van Trappen et al., 2002, 2004Zhu et al., 2003; Horn et al., 2005). The physiological characteristics of members of the genus Flavobacterium are also diverse: they can be psychrophilic, psychrotolerant or mesophilic; they can be halotolerant, halophilic or sensitive to salts; and they produce a variety of enzymes (Humphry et al., 2001;Tamaki et al., 2003;Aslam et al., 2005). These findings suggest that the genus Flavobacterium may have important environmental roles. Activated-sludge processes with cyclic changes of anaerobic and aerobic conditions have been used to remove phosphate from wastewater and are increasingly used to reduce the eutrophication process in lakes (Mino et al., 1987;Jeon & Park, 2000). An insight into the bacterial community responsible for phosphorus removal is a prerequisite for understanding and controlling the enhanced biological phosphorus removal mechanism and its processes. Therefore, efforts have been made in our laboratory to isolate and characterize members of the bacterial community found in activated sludge that perform enhanced biological phosphorus removal. Here we describe the taxonomic characterization of a novel species belonging to the genus Flavobacterium.Strain EMB47 T was isolated from activated sludge that was performing enhanced biological phosphorus removal in a laboratory-scale sequencing batch reactor. Sodium acetate was supplied as a sole carbon source, and the operation of the sequencing batch reactor was as described elsewhere (Jeon et al., 2003)....

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“…Physiological characteristics of Flavobacterium strains are also very diverse: they can be psychrophilic, psychrotolerant or mesophilic, they can be halotolerant, halophilic or sensitive to salts and they produce a variety of enzymes (Humphry et al, 2001;Tamaki et al, 2003;Aslam et al, 2005;Zhang et al, 2006), suggesting that they may have important roles in environmental habitats. Activated sludge processes have been used to remove organic compounds as well as nutrients from wastewater and insights regarding their bacterial communities are a prerequisite for understanding the mechanisms involved.…”

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Flavobacterium filum sp. nov., isolated from a wastewater treatment plant in Korea

Ryu

Park

Jeon

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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A Gram-negative bacterium, designated strain EMB34 T , was isolated from a wastewater treatment plant in Korea. Growth was observed between 10 and 40 6C (optimum, 25-35 6C) and between pH 6.0 and 9.5 (optimum, pH 7.5-8.0). The cells were non-motile rods, linked with extracellular fibrils. The predominant fatty acids of strain EMB34 T were iso-C 15 : 0 , C 15 : 0 , iso-C 15 : 1 G, iso-C 16 : 0 3-OH and iso-C 15 : 0 3-OH and the strain contained phosphatidylethanolamine and phosphatidylinositol as the polar lipids. The G+C content of the genomic DNA was 34.2 mol% and the major quinone was menaquinone-6. Comparative 16S rRNA gene sequence analyses showed that strain EMB34 T formed a distinct phyletic line within the genus Flavobacterium. The levels of 16S rRNA gene sequence similarity with other Flavobacterium species were less than 94.5 %. On the basis of phenotypic, chemotaxonomic and molecular data, it is clear that strain EMB34 T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium filum sp. nov. is proposed. The type strain is EMB34 T (5KCTC 12610 T 5DSM 17961 T ).Since the genus Flavobacterium, which belongs to the phylum Bacteroidetes, was established (Bergey et al., 1923), its description has been emended repeatedly and many novel Flavobacterium species have been described from diverse habitats such as micromats, fresh water and seawater, Antarctic lakes, soil, gut of an earthworm and sediments (Bernardet et al., 1996;McCammon & Bowman, 2000;Van Trappen et al., 2004; Kämpfer et al., 2006;Cousin et al., 2007). Physiological characteristics of Flavobacterium strains are also very diverse: they can be psychrophilic, psychrotolerant or mesophilic, they can be halotolerant, halophilic or sensitive to salts and they produce a variety of enzymes (Humphry et al., 2001;Tamaki et al., 2003;Aslam et al., 2005;Zhang et al., 2006), suggesting that they may have important roles in environmental habitats. Activated sludge processes have been used to remove organic compounds as well as nutrients from wastewater and insights regarding their bacterial communities are a prerequisite for understanding the mechanisms involved. Therefore, efforts have been made in our laboratory to isolate and characterize members of the bacterial community in activated sludge (Park et al., 2006(Park et al., , 2007. Here we describe the taxonomic characterization of a novel species belonging to the genus Flavobacterium.Strain EMB34 T was isolated from a wastewater treatment plant in Korea that performed enhanced biological phosphorus removal. The sludge sample was serially diluted with 1 % (w/v) saline solution, spread on R2A agar (Difco) and incubated at 20 u C for 5 days. The strain was grown routinely aerobically on R2A agar at 30 u C for 3 days.Sequencing of the 16S rRNA gene of strain EMB34 T was carried out as described previously by Lane (1991). The resulting 16S rRNA gene sequence (1436 nucleotides) was compared with available 16S rRNA gene sequences from GenBank using the BLAST program (http://...

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Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments

Abstract: NoteFlavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments

Cited by 102 publications

References 31 publications

Meiothermus hypogaeus sp. nov., a moderately thermophilic bacterium isolated from a hot spring

Meiothermus hypogaeus sp. nov., a moderately thermophilic bacterium isolated from a hot spring

Flavobacterium croceum sp. nov., isolated from activated sludge

Flavobacterium filum sp. nov., isolated from a wastewater treatment plant in Korea

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