Flavobacterium procerum sp. nov., isolated from freshwater

Abstract

A Gram-reaction-negative, strictly aerobic, yellow-pigmented, rod-shaped bacterium, designated strain T3T, was isolated from freshwater of Chishui River flowing through Maotai town, Guizhou, south-west China. Analysis of the16S rRNA gene sequence indicated that strain T3T was a member of the genus Flavobacterium and closely related to Flavobacterium resistens DSM 19382T (96.8 %). The novel strain was able to grow at 10-34 °C (optimum 28 °C), pH 6.0-11.0 (optimum pH 8.0-9.0) and with 0-2.0 % (w/v) NaCl (optimum 0 %). The major polar lipids were phosphatidylethanolamine, two unknown glycolipids, five unknown aminolipids and four unidentified lipids, and the major respiratory quinone was MK-6. The predominant fatty acids were C16 : 1ω7c and/or C16 : 1ω6c and iso-C15 : 0. The DNA G+C content of the strain was 36 mol %. Based on these data, strain T3T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium procerum sp. nov. is proposed. The type strain is T3T ( = CGMCC 1.12926T = JCM 30113T).

Flavobacterium lutivivi sp. nov., isolated from activated sludge

“…Cultural, morphological, physiological and biochemical tests were performed as described previously (Feng et al, 2015). Hydrolysis of casein, starch, gelatin, urea, CM-cellulose, tyrosine, lysine and ornithine, production of H 2 S and indole, and the Voges-Proskauer (VP) test were investigated according to previously described methods (Krieg & Padgett, 2011).…”

“…Chemotaxonomic analyses were performed as described previously (Feng et al, 2015). For cellular fatty acid and polar lipid analyses, cells of strain HQQ T and the four reference strains were grown in the same conditions on R2A agar at 25 8C for two days and were collected as described previously (Feng et al 2015).…”

“…For cellular fatty acid and polar lipid analyses, cells of strain HQQ T and the four reference strains were grown in the same conditions on R2A agar at 25 8C for two days and were collected as described previously (Feng et al 2015). Fatty acids were saponified, methylated and extracted from 40 mg of freeze-dried cells using the standard protocol of the MIDI system (Sherlock Microbial Identification System, version 6.1).…”

“…The number of species in the genus Flavobacterium is growing rapidly; at the time of writing, the genus comprised at least 150 species with validly published names (http://www.bacterio.cict.fr/). Species of the genus Flavobacterium have been isolated from a number of diverse habitats, such as freshwater (Kim et al, 2009;Lee et al, 2011;Kacagan et al, 2013;Chen et al, 2013;Feng et al, 2015), sediments (Qu et al, 2009;Fu et al, 2011;Kaur et al, 2012), plants (Kuo et al, 2013), animals (Glaeser et al, 2013) and Antarctic lakes (McCammon et al, 1998;Humphry et al, 2001;Yi et al, 2005). Activated sludge is rich in micro-organisms and two novel species, Flavobacterium croceum and Flavobacterium defluvii (Park et al, 2006(Park et al, , 2007, have been isolated from activated sludge in recent years.…”