Flavobacterium selenitireducens sp. nov., isolated from rhizosphere soil of ancient mulberry

Abstract

A Gram-stain-negative, non-motile, aerobic, yellow, convex, rod-shaped mesophilic bacterial strain, designated strain D33T, was isolated from rhizosphere soil of ancient mulberry in Dezhou city, Shandong province, PR China. The strain grew at 8–37 °C (optimum, 30 °C), pH 4–9 (optimum, pH 7) and growth occurred at 0.5–5.5 % (w/v) NaCl (optimally at 1 %). The results of the phylogenetic analyses of 16S rRNA gene and whole genome sequences indicated that D33T was closely related to members of the genus Flavobacterium and had the highest 16S rRNA gene sequence similarity with ‘Flavobacterium agri’ KACC 19300 (95.4 %), Flavobacterium ichthyis NST-5T (94.6 %), Flavobacterium ahnfeltiae KCTC 32467T (93.6 %) and Flavobacterium longum JCM 19141T (93.6 %). The genome size of D33T was 3.8 Mb and the DNA G+C content was 48.0 mol%. The average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH) and average amino acid identity (AAI) values among D33T and reference strains were lower than the threshold values for species delineation. The only respiratory quinone of D33T was menaquinone 6 (MK-6). The predominant fatty acids (>5 %) were C15:0, C16 : 0, C18 : 0, iso-C15:0, iso–C17 : 0 3-OH, anteiso-C15 : 0 and summed feature 9 . The polar lipid profile contained phosphatidylethanolamine, two unidentified aminophospholipids, three unidentified aminolipids and two unidentified lipids. Combined data from phenotypic, phylogenetic and chemotaxonomic studies indicated that D33T is a representative of a novel species of the genus Flavobacterium, for which the name Flavobacterium selenitireducens sp. nov. is proposed. The type strain is D33T (=GDMCC 1.1946T=KACC 22131T).

Flavobacterium okayamense sp. nov. isolated from surface seawater

“…This genus contains at least 314 species with legitimately published names at the time of writing ( https://lpsn.dsmz.de/ , April 2023). Flavobacterium species have been recognized in a wide variety of biotopes, including soil (Máté et al 2022 ; Hou et al 2022 ), river water (Watanabe et al 2022 ), plants (Seo et al 2022 ), seawater (Sun et al 2022 ), sites in Antarctica (Králová et al 2021 ), and chinstrap penguins (Irgang et al 2023 ). Physiologically, cells of the genus Flavobacterium are typically Gram-stain-negative, rod-shaped, aerobic, and heterotrophic, and they yield yellow or orange colonies (McBride 2014 ).…”