Flavocoxid attenuates airway inflammation in ovalbumin-induced mouse asthma model

Abdеlaziz, Rania R.; ElMahdy, Mohammed Kh.; Suddеk, Ghada M.

doi:10.1016/j.cbi.2018.07.001

Cited by 25 publications

(24 citation statements)

References 53 publications

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“…OVA challenge led to a significant elevation in the number of total white blood cells, lymphocytes, monocytes, and eosinophils in BALF, confirming the previous study in which OVA induced significant elevation of cell counts in BALF mice [20,27]. e current results showed that OVAinduced increases of BALF total cells, total leukocytes, lymphocytes, eosinophils, and monocytes were significantly and dose-dependently inhibited by oral treatment with PS + CR (300 and 1,000 mg/kg), as being comparable to the effects of DEXA (3 mg/kg), as antiinflammatory activity that mediates antiasthmatic effects.…”

Section: Discussionsupporting

confidence: 88%

“…e main pathological changes that highlight asthma are mucus overproduction, airway obstruction, and increased infiltration of inflammatory cells, including mast cells, eosinophils, and lymphocytes [48]. Histopathologically, marked sarcomatous changes, hyperplasia of mucous producing cells in bronchioles, thickening of alveolar septum, and infiltration of inflammatory cells, mast cells, and eosinophils, were observed in OVA-challenged mouse lung tissues [20,21,27] and also in our OVA control mice. e ASA (%/mm 2 ) reflected the pulmonary functions, gas exchange capacities, and decreases of ASA indicated a reduction of the lung gas exchange surface, that is, the decreases of lung capacities in various lung diseases, including asthma [20,28].…”

Section: Discussionmentioning

confidence: 64%

“…Evidence-Based Complementary and Alternative Medicine mechanical studies (i.e., transforming growth factors and mitogen-activated protein kinases) and more plentiful in vivo studies should be tested in future [40,41]. Challenge with OVA induced significant airways architectural and physiological changes [27]. Biochemically, lung weight index, that is, relative weight to body weight, is considered a good marker of vascular permeability and fdfma [42].…”

Section: Discussionmentioning

confidence: 99%

“…Approximately equal regions of the left lateral lobe of an individual lung were cross-trimmed according to previously established methods [25]. Representative sections were stained with hematoxylin and eosin (H&E) for general histopathology, Congo red for eosinophils, or toluidine blue for mast cells, according to previously established methods [20,26,27]. After that, the histological profiles of individual cross-trimmed left lateral lobes of lung tissue were observed by using a light microscope (Model Eclipse 80i, Nikon, Tokyo, Japan) while being blinded to group distribution.…”

Section: Lung Samplingmentioning

confidence: 99%

“…After that, the histological profiles of individual cross-trimmed left lateral lobes of lung tissue were observed by using a light microscope (Model Eclipse 80i, Nikon, Tokyo, Japan) while being blinded to group distribution. To observe more details of any changes, the mean ASA (%/mm 2 ) reflected the pulmonary functions, gas exchange capacities [20,28], and mean alveolar septal thickness (μm) and numbers of inflammatory cells, eosinophils, and mast cells infiltrated in the alveolar regions (cells/mm 2 ) [20,21,27] were calculated for histomorphometrical analysis using a computerassisted image analysis program (iSolution FL ver 9.1, IMT i-Solution Inc., Vancouver, Quebec, Canada).…”

Section: Lung Samplingmentioning

confidence: 99%

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Effects of Pelargonium sidoides and Coptis Rhizoma 2 : 1 Mixed Formula (PS + CR) on Ovalbumin‐Induced Asthma in Mice

Min

Park

Choi

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Pelargonium sidoides (PS) is traditionally used to treat respiratory and gastrointestinal infections, dysmenorrhea, and hepatic disorders in South Africa. Coptis Rhizoma (CR) is used to treat gastroenteric disorders, cardiovascular diseases, and cancer in East Asia. In the present study, we intended to observe the possible beneficial antiasthma effects of PS and CR on the ovalbumin- (OVA-) induced asthma C57BL/6J mice. Asthma in mice was induced by OVA sensitization and subsequent boosting. PS + CR (300 and 1,000 mg/kg; PO) or dexamethasone (IP) was administered once a day for 16 days. The changes in the body weight and gains, lung weights and gross inspections, total and differential cell counts of leukocytes in bronchoalveolar lavage fluid (BALF), serum OVA-specific immunoglobulin E (OVA-sIgE) levels, interleukin-4 (IL-4) and IL-5 levels in BALF and lung tissue homogenate, and IL-4 and IL-5 mRNA levels in lung tissue homogenates were analyzed with lung histopathology: mean alveolar surface area (ASA), alveolar septal thickness, numbers of inflammatory cells, mast cells, and eosinophils infiltrated in the alveolar regions, respectively. Significant increases in lung weights, total and differential cell counts of leukocytes in BALF, serum OVA-sIgE levels, and IL-4 and IL-5 levels in BALF and lung tissue homogenate were observed in OVA control as compared to those of intact control. In addition, OVA control showed a significant decrease in mean ASA and increases in alveolar septal thickness, numbers of inflammatory cells, mast cells, and eosinophils infiltrated in alveolar regions. However, these allergic and inflammatory asthmatic changes were significantly inhibited by PS + CR in a dose-dependent manner. In this study, PS + CR showed dose-dependent beneficial effects on OVA-induced asthma in mice through anti-inflammatory and antiallergic activities. Therefore, it is expected that PS + CR have enough potential as a new therapeutic agent or as an ingredient of a medicinal agent for various allergic and inflammatory respiratory diseases including asthma.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Lung Samplingmentioning

confidence: 99%

Section: Lung Samplingmentioning

confidence: 99%

See 3 more Smart Citations

Effects of Pelargonium sidoides and Coptis Rhizoma 2 : 1 Mixed Formula (PS + CR) on Ovalbumin‐Induced Asthma in Mice

Min

Park

Choi

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Therapeutic efficacy of spirulina against ovalbumin and cigarette smoke‐induced asthma‐specific stress biomarkers in Sprague–Dawley rats

Riaz

Butt

Sharif

et al. 2022

Food Science & Nutrition

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Due to the high prevalence of allergies and asthma, awareness about allergens and therapeutic use of functional foods and nutraceuticals have gained immense attention. Spirulina powder is being used as health‐boosting and antioxidant agent against several ailments owing to its unique nutritional profile. Considering its antioxidant role, the current study was focused on exploring therapeutic role of spirulina against stress biomarkers in asthmatic model. To assess the therapeutic efficacy of spirulina against allergic asthma‐specific oxidative stress biomarkers, a model feed trial was conducted and rats were divided into four groups ( n = 10). G 0–I (negative control), G 0–II (positive control), whereas G I (spirulina) and G 2 (salbutamol) served as treatment groups. Salbutamol is a chemical compound which is used in several antiallergic medicines because it works as bronchodilator. G 2 group was given salbutamol for comparison of results. For asthma induction, rats were given intraperitoneal injection of ovalbumin on 7th, 14th, and 21st day. Treatment groups were given spirulina powder (500 mg/kg body weight) and salbutamol (1 mg/kg), respectively, after the induction of asthma. All three asthmatic groups were also exposed to cigarette smoke daily along with respective treatment for 4 weeks. Asthma induction caused an increase in total cell count in bronchioalveolar fluid (BALF), while spirulina treatment reduced total cells in BALF by 33.50% and salbutamol by 41.7%. Level of interleukins (IL) like IL‐4 decreased by 33.32% & 48.56% in G 1 and G 2 . Similarly, IL‐5 and IL‐13 levels reduced by 40.9% & 49.9% and 18.62% & 38.02%, respectively, in G 1 and G 2 . Serum levels of Immunoglobin‐E (Ig‐E) declined by 29.70% and 52.82%, while histamine levels were 26.23% & 45.58% less at the end of study in comparison to positive control. Moreover, histological analysis of lung tissue revealed that both spirulina and salbutamol effectively reduced ovalbumin and cigarette smoke‐induced moderate to severe necrosis, architectural changes, and congestion. It was concluded that salbutamol showed better results however, spirulina also effectively reduced mild to moderate allergic symptoms in dose‐dependent manner. Nutraceutical and functional foods are considered helpful in mitigating oxidative stress‐mediated health problems. Spirulina has its unique nutritional profile including phycobiliproteins, phytochemicals, and antioxidant vitamins which make it useful against several ailments. Considering its antioxidant role, current study was focused on exploring therapeutic efficacy of spirulina against stress biomarkers in asthmatic model. Outcomes of present research also demonstrated beneficial effect of spirulina in modulating allergic symptoms. In this regard, ancient concept of ...

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Fingolimod attenuates ovalbumin‐induced airway inflammation via inhibiting MAPK/ERK signaling in mice

Makled

El‐Sheakh

2022

J Biochem & Molecular Tox

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The current study was designed to investigate the potential anti-inflammatory and antioxidant effects of fingolimod against Ovalbumin (Ova)-induced allergic airway inflammation compared to dexamethasone. Fingolimod was given (0.5 mg/kg/day, p.o.) for sensitized mice 1 h before Ova challenge from Days 19 to 24. Fingolimod significantly inhibited Ova-induced elevation of inflammatory cells and eosinophils numbers in bronchoalveolar lavage fluid (BALF) and reduced concentrations of immunoglobulin E in serum and of sphingosine-1-phosphate, interleukin (IL)-4, and IL-13 in BALF. Fingolimod inhibited microvascular leakage and edema as reflected by the decreased lung/body weight index. These findings were supported by histopathological examination results showing that fingolimod substantially decreased perivascular edema and inflammatory cell infiltration. Fingolimod also attenuated Ova-induced oxidative stress as evidenced by decreased malondialdehyde concentration along with increasing concentrations of reduced glutathione and superoxide dismutase in lung tissues. Fingolimod also significantly decreased monocyte chemoattractant protein-1 (MCP-1), p-ERK, and p-P38 in lung tissues of Ova-challenged mice. In conclusion, the current study demonstrated the anti-inflammatory and antioxidant effects of fingolimod in allergic airway inflammation that might be associated with the downregulation of mitogen activated kinases signaling to decrease T helper 2 cytokine secretion (IL-4 and IL-13) and MCP-1 expression, along with the inhibition of oxidative stress.

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Flavocoxid attenuates airway inflammation in ovalbumin-induced mouse asthma model

Cited by 25 publications

References 53 publications

Effects of Pelargonium sidoides and Coptis Rhizoma 2 : 1 Mixed Formula (PS + CR) on Ovalbumin‐Induced Asthma in Mice

Effects of Pelargonium sidoides and Coptis Rhizoma 2 : 1 Mixed Formula (PS + CR) on Ovalbumin‐Induced Asthma in Mice

Therapeutic efficacy of spirulina against ovalbumin and cigarette smoke‐induced asthma‐specific stress biomarkers in Sprague–Dawley rats

Fingolimod attenuates ovalbumin‐induced airway inflammation via inhibiting MAPK/ERK signaling in mice

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