Flavonoid Accumulation Is Correlated with Adventitious Roots Formation in Eucalyptus gunnii Hook Micropropagated through Axillary Bud Stimulation

Abstract

Eucalyptus gunnii Hook microcuttings, obtained in vitro through axillary bud stimulation, show different rooting responses on the same rooting medium depending on the physiological state induced by cytokinins used in the previous multiplication medium. 6-Furfurylamino purine and 6-(4-hydroxy-3-methylbut-2-enylamino)purine induced a physiological state characterized by high sensitivity of microcuttings to the rooting stimulus exerted by the auxin 3-indolebutyric acid, but N6-benzyladenine did not produce the same effect. The former physiological state was characterized by an increased accumulation of two endogenous flavonoids (identified as quercetin glycosides) which may be markers of a well defined physiological state. They could have some direct influence on the rooting processes of the explants cultivated in vitro.

Quantitative trait loci mapping for vegetative propagation in pedunculate oak

“…It is a complex process, which is affected by multiple factors including levels of growth regulators [23] and of phenolic compounds [12], nutritional status, associated stress responses such as wounding, and genetic characteristics. In oak the ability for vegetative propagation was found to be controlled by at least 6 unique QTLs, collectively explaining 53.9% of the phenotypic variation; two individual QTLs explaining more than 10% of the phenotypic variance were found over consecutive years.…”

Review of forests, wood products and wood biotechnology of Iran and Germany - Part III

“…The rooting response were correlated with the production of particular flavonoids under the influence of the cytokinin in the shoot multiplication medium (Curir, 1990). Endogenous cytokinins apper to have a role in adventitious root production.…”

“…Roots could be induced on shoots taken from multiplication media containing Kin or Zeatin but not BAP. Curir et al (1990) reported for E.gunnii Hook. …”

Developmental role of phenylalanine-ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) genes during adventitious rooting of Juglans regia L. microshoots

“…Our finding is consistent with those of Jay-Allemand et al [26] who reported that the promoting role of quercitrin (a glycone form of quercetin) during the root induction of Juglan regia microcuttings. Curir et al [11] also suggested that quercetin derivatives in the living tissue of Eucalypts gunnii, take part in developmental processes of microcutting rooting. Encouraging affect of quercetin on the formation of adventitious roots of Ilex paraguariensis cuttings was stated by Tarrago et al [46].…”