Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA’s Profile and Cell Morphology in Human Colon Cancer Cell Lines

Tutino, Valeria; Gigante, Isabella; Milella, Rosa Anna; Nunzio, Valentina De; Flamini, Riccardo; Rosso, Mirko De; Scavo, Maria Principia; Depalo, Nicoletta; Fanizza, Elisabetta; Caruso, Maria Gabriella; Notarnicola, Maria

doi:10.3390/molecules25153352

Cited by 9 publications

(13 citation statements)

References 52 publications

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“…Polyphenolic profiles were determined by Ultra‐High‐Performance Liquid Chromatography/Quadrupole‐Time of Flight mass spectrometry (UHPLC/QTOF) analysis as previously the study reported. [ 49 ]…”

Section: Methodsmentioning

confidence: 99%

Impact of Fresh Table Grape Intake on Circulating microRNAs Levels in Healthy Subjects: A Significant Modulation of Gastrointestinal Cancer‐Related Pathways

Tutino

Nunzio

Milella

et al. 2021

Molecular Nutrition Food Res

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Scope The study aims to investigate the effects of fresh table grape consumption in healthy subjects on circulating levels of the most common human microRNAs (miRNAs). The regulatory network governed by these modulated miRNAs is also investigated. Methods and Results Autumn Royal table grape, used in this study, is chosen for its high polyphenolic content and antioxidant properties. The study is a randomized controlled trial, in which 40 consecutive subjects are recruited on a voluntary basis and randomly assigned to two groups of the study, the control group, receiving only dietary recommendations and a grape group receiving a daily dose of 5 g of fresh table grape per kg of body weight for 21 days. All analyses are performed at baseline and after 21 days of dietary treatment. Circulating miRNAs levels are detected by Real‐Time quantitative PCR (RT‐qPCR) followed by bioinformatic functional analysis. The study identifies 20 circulating miRNAs differentially expressed in healthy subjects after grape intake, and in particular, 18 of 20 are down‐regulated and 2 are up‐regulated. Conclusion The dietary intake of table grape affects circulating miRNAs levels in healthy subjects, particularly the miRNAs related to pathways involved in counteracting cancer development, including gastrointestinal cancers.

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Section: Methodsmentioning

confidence: 99%

Impact of Fresh Table Grape Intake on Circulating microRNAs Levels in Healthy Subjects: A Significant Modulation of Gastrointestinal Cancer‐Related Pathways

Tutino

Nunzio

Milella

et al. 2021

Molecular Nutrition Food Res

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“…The polyphenolic composition of grapes, which depends on genotype, environmental factors and agronomic practices [ 104 ], affects the structural components of the cytoskeleton, cellular adhesions, and the cell membrane fatty acids profile [ 104 ]. Briefly, Figure 4 shows the main results obtained by our studies on grape skin and grape pomace extracts, as below.…”

Section: Fruit Componentsmentioning

confidence: 99%

“…The results of in vitro studies highlighted that Autumn Royal and Egnatia grape skin extracts influenced the gene expression of 15-LOX-1 and PPAR-γ. In particular, grape skin extracts polyphenols induced a significant upregulation of both 15-LOX-1 and PPAR-γ [ 104 ]. Together, these activated factors are required for the inhibition of the colorectal tumorigenesis process.…”

Section: Fruit Componentsmentioning

confidence: 99%

Nutraceuticals: Focus on Anti-Inflammatory, Anti-Cancer, Antioxidant Properties in Gastrointestinal Tract

et al. 2022

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In recent years, nutraceuticals have gained great popularity, owing to their physiological and potential health effects, such as anti-inflammatory, anti-cancer, antioxidant, and prebiotic effects, and their regulation of lipid metabolism. Since the Mediterranean diet is a nutritionally recommended dietary pattern including high-level consumption of nutraceuticals, this review aimed to summarize the main results obtained by our in vitro and in vivo studies on the effects of the major constituents of the Mediterranean diet (i.e., extra virgin olive oil compounds, polyunsaturated fatty acids, and fruit components). Based on experimental studies, the therapeutic purpose of nutraceuticals depends on their bioavailability, solubility, toxicity, and delivery system. This review provides more in-depth knowledge on the effects linked to nutraceuticals administration on human health, focusing the gastrointestinal tract and suggesting specific dietary components for personalized adjuvant therapies.

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“…Experimental in vitro studies have shown an increase in total polyunsaturated fatty acids (PUFAs) levels in cells treated with Autumn Royal table grape extracts [ 11 , 12 ]. In particular, compared to untreated control cells, the grape extracts caused an increase in essential fatty acids (EFAs), namely linoleic acid (LA) and α-linolenic acid (ALA).…”

Section: Introductionmentioning

confidence: 99%

“…In particular, compared to untreated control cells, the grape extracts caused an increase in essential fatty acids (EFAs), namely linoleic acid (LA) and α-linolenic acid (ALA). In addition, in cell membranes, the grape treatment exerted a significant increase in total PUFAs, associated with changes in their downstream metabolic pathways [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Beneficial Effects of Table Grape Use on Serum Levels of Omega-3 Index and Liver Function: A Randomized Controlled Clinical Trial

et al. 2022

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This clinical trial was aimed to investigate the effects of fresh table grape intake on the serum levels of the Omega-3 index, defined as the sum of eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA) levels. Forty consecutive healthy subjects were randomly assigned to the control group, receiving only dietary recommendations, and the grape group receiving a daily dose of 5 g of fresh table grape per kg of body weight, for 21 days. Compared with baseline, the grape treatment produced no significant difference in the serum levels of glucose, liver transaminase, and triglycerides, with the exception of cholesterol value, which was significantly reduced in both control and grape group (180.5 ± 20.32 vs. 196.1 ± 30.0 and 181.4 ± 21.9 vs. 194.3 ± 37.5, respectively). After 4 weeks from the end of grape treatment, the analysis of single fatty acids showed a significant increase in oleic acid content (14.15 ± 1.8 vs. 12.85± 1.6, p < 0.05) and a significant induction of the Omega-3 index (8.23 ± 1.9 vs. 6.09 ± 1.2, p < 0.05), associated with increased serum levels of adiponectin (24.09 ± 1.08 vs. 8.8 ± 0.7, p < 0.001). In contrast, the expression of fibroblast growth factor 21 (FGF21), a molecule associated with metabolic syndrome and liver disease, was significantly reduced (37.9 ± 6.8 vs. 107.8 ± 10.1, p < 0.001). The data suggest that the intake of fresh grape improves the Omega-3 index in the serum and exerts beneficial effects on liver function through the overexpression of adiponectin and the reduction in FGF21 levels.

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Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA’s Profile and Cell Morphology in Human Colon Cancer Cell Lines

Cited by 9 publications

References 52 publications

Impact of Fresh Table Grape Intake on Circulating microRNAs Levels in Healthy Subjects: A Significant Modulation of Gastrointestinal Cancer‐Related Pathways

Impact of Fresh Table Grape Intake on Circulating microRNAs Levels in Healthy Subjects: A Significant Modulation of Gastrointestinal Cancer‐Related Pathways

Nutraceuticals: Focus on Anti-Inflammatory, Anti-Cancer, Antioxidant Properties in Gastrointestinal Tract

Beneficial Effects of Table Grape Use on Serum Levels of Omega-3 Index and Liver Function: A Randomized Controlled Clinical Trial

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