Flavonoid biosynthesis in Dianthus caryophyllus L. is early regulated during interaction with Fusarium oxysporum f. sp. dianthi

Romero-Rincón, Ana; Martínez, Sixta Tulia; Higuera, Blanca Ligia; Coy-Barrera, Ericsson; Ardila, Harold

doi:10.1016/j.phytochem.2021.112933

Cited by 11 publications

(6 citation statements)

References 86 publications

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“…Phenylpropanoids are known phytoalexins reported across different plant families, whose biosynthesis is induced by fungal infection . In grapes, nitrous oxide-induced accumulation of phenylpropanoids has been reported to enhance resistance to Botrytis cinerea, significantly reducing the diameter and incidence of lesions during post-harvest .…”

Section: Discussionmentioning

confidence: 99%

“…Phenylpropanoids are known phytoalexins reported across different plant families, 47 whose biosynthesis is induced by fungal infection. 48 In grapes, nitrous oxide-induced accumulation of phenylpropanoids has been reported to enhance resistance to Botrytis cinerea , significantly reducing the diameter and incidence of lesions during post-harvest. 49 With direct antifungal activity, phenylpropanoids may also regulate ROS levels during infection by necrotrophic pathogens 50 rather than the modulation of hormonal profile to support a more effective response to the pathogen.…”

Section: Discussionmentioning

confidence: 99%

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Metabolomics Insight into the Variety-Mediated Responses to Aspergillus carbonarius Infection in Grapevine Berries

Giorni,

Zhang,

Bavaresco

et al. 2023

ACS Omega

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Limited knowledge regarding the susceptibility of grape varieties to ochratoxin A (OTA)-producing fungi is available to date. This study aimed to investigate the susceptibility of different grape varieties to Aspergillus carbonarius concerning OTA contamination and modulation at the metabolome level. Six grape varieties were selected, sampled at early veraison and ripening, artificially inoculated with A. carbonarius, and incubated at two temperature regimes. Significant differences were observed across cultivars, with Barbera showing the highest incidence of moldy berries (around 30%), while Malvasia and Ortrugo showed the lowest incidence (about 2%). OTA contamination was the lowest in Ortrugo and Malvasia, and the highest in Croatina, although it was not significantly different from Barbera, Merlot, and Sauvignon Blanc. Fungal development and mycotoxin production changed with grape variety; the sugar content in berries could also have played a role. Unsupervised multivariate statistical analysis from metabolomic fingerprints highlighted cultivar-specific responses, although a more generalized response was observed by supervised OPLS-DA modeling. An accumulation of nitrogen-containing compounds (alkaloids and glucosinolates), phenylpropanoids, and terpenoids, in addition to phytoalexins, was observed in all samples. A broader modulation of the metabolome was observed in white grapes, which were less contaminated by OTA. Jasmonates and oxylipins were identified as critical upstream modulators in metabolomic profiles. A direct correlation between the plant defense machinery and OTA was not observed, but the information was acquired and can contribute to optimizing preventive actions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Metabolomics Insight into the Variety-Mediated Responses to Aspergillus carbonarius Infection in Grapevine Berries

Giorni,

Zhang,

Bavaresco

et al. 2023

ACS Omega

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“…The inoculation of carnation cuttings with Fod was carried out in accordance with the conditions reported by (Romero-Rincón et al, 2021;Santos-Rodríguez et al, 2021;Vanegas-Cano et al, 2022). All the cuttings (with and without the addition of the inducer) were kept in optimal conditions of humidity (average 59.6%), temperature (average 18.6°C), light, and nutrition.…”

Section: Effect Of Thiamine Concentration As An Inducer Of Resistancementioning

confidence: 99%

Response of susceptible carnation cultivars during thiamine application as inducer of resistance to vascular wilting caused by Fusarium oxysporum f. sp. dianthi

Mora,

Melgarejo,

Ardila

2024

Ornam. Hortic.

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Colombia is one of the main producers of carnations worldwide. However, its production has been affected by vascular wilting caused by the pathogen Fusarium oxysporum f. sp. dianthi (Fod). In previous studies, thiamine has been proposed as a potential resistance inducer for this disease, under greenhouse growth conditions. However, the specific conditions for its application must be studied when applying it under commercial conditions. In this sense, this study aimed to evaluate the effect of the thiamine foliar spray in different concentrations among some susceptible carnation cultivars in response to Fod-inoculation. For this, two in-vivo trials were carried out, using foliar spraying of thiamine as a treatment before inoculation with the pathogen Fod, then measurements of incidence, severity, and the content of phenolic and flavonoid compounds were made. It was found that thiamine application, in the concentrations evaluated, shows a statistically similar decrease in the disease incidence and severity. These results were verified using multivariate statistical analysis. In addition, it was found that the application of thiamine 1 mmol L-1 presents a positive response in reducing the effects of the disease in three susceptible cultivars. The results suggest that thiamine has the potential to be applied as a technique in the integrated management of disease in the productive sector.

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“…Hence, further studies are required to determine whether flavonol acylation might be related to carnation resistance. Indeed, previous studies described that Fod-resistant carnation cultivars can accumulate flavonoids generally glycosylated [29], [42], [52] or acylglycosylated [53], [54]. Certainly, flavonoids can have structural variations (e.g., methylation by methyltransferases, glycosylation by glycosyltransferases or acylation by specific acyltransferases), which modify flavonoid stability, solubility, and reactivity.…”

Section: Metabolic Profiling Of the Apoplastic Fluid Of Two Cultivars...mentioning

confidence: 99%

“…Contrarily, the susceptible cultivar was the glycosylated dihydroflavonol 4, which seems to be an intermediate that did not reach the flavonol end-product, possibly due to a reduced flavonol synthase (FLS) activity. Transcriptional studies have shown that the FLS exhibited lower levels in Fod-susceptible carnation cultivars than in those resistant cultivars [53]. Partial least squares-discriminant analysis (PLS-DA) favored extending the previous comparisons.…”

Section: Metabolic Profiling Of the Apoplastic Fluid Of Two Cultivars...mentioning

confidence: 99%

Extracción y análisis de metabolitos fenólicos apoplásticos en raíz y tallo de clavel (Dianthus caryophyllus L)

2022

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En el presente estudio se describe el acondicionamiento de algunos parámetros con fines de obtención eficiente de extractos apoplásticos enriquecidos en compuestos polares, principalmente fenólicos. Este flujo de trabajo descrito, incluso, puede ser aplicado a diferentes especies vegetales para ser empleado en el análisis particular o global de metabolitos en este espacio extracelular periférico. Para ello, usando raíces y tallos de clavel (Dianthus cariophyllus L), se evaluaron diferentes soluciones de infiltración para la extracción de los metabolitos apoplásticos. El mejor resultado se logró con la disolución amortiguadora NaH2PO4-Na2HPO4 0,1 M pH 6,5/NaCl 50 mM, porque se obtiene la mayor cantidad de metabolitos fenólicos apoplásticos, con la menor contaminación de compuestos intracelulares. Los metabolitos se separaron mediante HPLC-DAD-ESI-MS, obteniendo perfiles cromatográficos con parámetros de calidad razonables basados en resolución, selectividad y número de platos teóricos. Con estas condiciones, fue posible identificar ocho compuestos diferenciales (una flavona y siete flavonoles), cuyas estructuras básicas comprendían flavonoides del tipo (iso)pratol, kaempférido, (dihidro)kaempferol, quercetina y miricetina, según el órgano de prueba y la variedad. Los flavonoides identificados están relacionados con metabolitos de tipo fitoanticipina en el clavel, como hidroxi-metoxiflavona, di-o-benzoilquercetina y kaempférido disaliciloilrhamnósido, abundantemente presentes en la variedad resistente. Las condiciones descritas en este trabajo son fundamentales para profundizar en el papel de los metabolitos fenólicos apoplásticos relacionados con los mecanismos de defensa de esta planta ornamental.

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Flavonoid biosynthesis in Dianthus caryophyllus L. is early regulated during interaction with Fusarium oxysporum f. sp. dianthi

Cited by 11 publications

References 86 publications

Metabolomics Insight into the Variety-Mediated Responses to Aspergillus carbonarius Infection in Grapevine Berries

Metabolomics Insight into the Variety-Mediated Responses to Aspergillus carbonarius Infection in Grapevine Berries

Response of susceptible carnation cultivars during thiamine application as inducer of resistance to vascular wilting caused by Fusarium oxysporum f. sp. dianthi

Extracción y análisis de metabolitos fenólicos apoplásticos en raíz y tallo de clavel (Dianthus caryophyllus L)

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