Flavonoid-derived anisotropic silver nanoparticles inhibit growth and change the expression of virulence genes in <i>Escherichia coli</i> SM10

Osonga, Francis J.; Akgül, Ali; Yazgan, İ̇dris; Akgul, Ayfer; Ontman, Renata; Kariuki, Victor M.; Eshun, Gaddi B.; Sadik, Omowunmi A.

doi:10.1039/c7ra13480k

Cited by 26 publications

(29 citation statements)

References 45 publications

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“…However, smaller and round-shaped nanoparticles (1–4 nm) were found to be in higher densities than the long sheets of Pep-AgNPs (40–80). A similar distribution was observed by the flavonoid-derived anisotropic silver nanoparticles [ 29 ]. The different shape and size of the Pep-AgNPs are mainly due to the peptone chemical composition.…”

Section: Resultssupporting

confidence: 79%

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Green-Synthesis of Anisotropic Peptone-Silver Nanoparticles and Its Potential Application as Anti-Bacterial Agent

et al. 2019

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This study demonstrates a green-route-based synthesis of high-concentration suspensions of anisotropic silver nanoparticles (AgNPs) by peptone (Pep), a soluble protein hydrolysate and an abundantly used nutrient source in microbial-media. The transformation of Ag ions from solution into a high-concentration suspension of anisotropic Pep-AgNPs, at an extremely low concentration of peptone (0.02%), indicates that the present green-route synthesis method follows “low volume high concentration nano-synthesis”, and, hence, enhances the economic significance of the process. Process optimization with different concentrations of AgNPs (1–5 mM), NaOH solution (5–40 mM), and peptone (0.004%–0.12%) gave the optimized Pep-AgNPs synthesis at 3 mM of AgNO3, 20 mM of NaOH, and 0.02% of the peptone concentrations. The green-route synthesized Pep-AgNPs were structurally characterized by the TEM, XPS, FT-IR, and XRD analyses. The Pep-AgNPs against the clinically relevant bacteria Escherichia coli and Staphylococcus aureus gave significant anti-bacterial properties, with a MIC (minimum inhibitory concentration) of 100 ppm. The colony counting and morphological observation of the bacterial cell under SEM corroborated an anti-bacterial potential of the Pep-AgNPs. Therefore, Pep-AgNPs are green-route synthesized, anisotropic, and have a significant anti-bacterial potential that can be used in many relevant applications.

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Section: Resultssupporting

confidence: 79%

“…The TEM image showed a combination of long sheets and very small round-shaped Pep-AgNPs nano-particles. The anisotropic morphology shows different sizes and various shapes of the nanoparticle [ 27 , 28 , 29 ]. Therefore, the obtained TEM image corroborated that Pep-AgNPs has anisotropic particle morphologies.…”

Section: Resultsmentioning

confidence: 99%

Green-Synthesis of Anisotropic Peptone-Silver Nanoparticles and Its Potential Application as Anti-Bacterial Agent

et al. 2019

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“…Previous studies have shown that AgNPs derived from phosphorylated flavonoids exhibited excellent antibacterial activity [25,51]. In addition to size, the shape also showed the importance of similar-sized LTP-AgNPs, where quasi-spherical LTP-AgNPs showed higher capability (LTP-AgNP5) than corresponding similar-sized spherical ones.…”

Section: Discussionmentioning

confidence: 92%

Size and Shape-Dependent Antimicrobial Activities of Silver and Gold Nanoparticles: A Model Study as Potential Fungicides

et al. 2020

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Plant-based pathogenic microbes hinder the yield and quality of food production. Plant diseases have caused an increase in food costs due to crop destruction. There is a need to develop novel methods that can target and mitigate pathogenic microbes. This study focuses on investigating the effects of luteolin tetraphosphate derived silver nanoparticles (LTP-AgNPs) and gold nanoparticles (LTP-AuNPs) as a therapeutic agent on the growth and expression of plant-based bacteria and fungi. In this study, the silver and gold nanoparticles were synthesized at room temperature using luteolin tetraphosphate (LTP) as the reducing and capping agents. The synthesis of LTP-AgNPs and LTP-AuNP was characterized by Transmission Electron Microscopy (TEM) and size distribution. The TEM images of both LTP-AgNPs and LTP-AuNPs showed different sizes and shapes (spherical, quasi-spherical, and cuboidal). The antimicrobial test was conducted using fungi: Aspergillus nidulans, Trichaptum biforme, Penicillium italicum, Fusarium oxysporum, and Colletotrichum gloeosporioides, while the class of bacteria employed include Pseudomonas aeruginosa, Aeromonas hydrophila, Escherichia coli, and Citrobacter freundii as Gram (−) bacteria, and Listeria monocytogenes and Staphylococcus epidermidis as Gram (+) bacterium. The antifungal study demonstrated the selective size and shape-dependent capabilities in which smaller sized spherical (9 nm) and quasi-spherical (21 nm) AgNPs exhibited 100% inhibition of the tested fungi and bacteria. The LTP-AgNPs exhibited a higher antimicrobial activity than LTP-AuNPs. We have demonstrated that smaller sized AgNPs showed excellent inhibition of A. nidulans growth compared to the larger size nanoparticles. These results suggest that LTP-AuNP and LTP-AgNPs could be used to address the detection and remediation of pathogenic fungi, respectively.

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“…The synthesized Ag NPs demonstrated a reducing effect on the swarming behaviour of E. coli, P. aeruginosa PAO1, and K. pneumoniae [80]. Also, Ag NPs showed to have reduced about 98% activity of the swarming motility associated with the arresting effect in the expression of fliL flagellar gene in E. coli [81]. The swarming movement was the reason of combination sensory transduction and universal control operations.…”

Section: The Impact Of Nanoparticles On Swarming Phenomenonmentioning

confidence: 91%

Down-Regulation of fliL Gene Expression by Ag Nanoparticles and TiO2 Nanoparticles in Pragmatic Clinical Isolates of Proteus mirabilis and Proteus vulgaris from Urinary Tract Infection

Saleh¹,

Malik²,

Al-Rubaii³

2019

Nano BioMed ENG

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Ten isolates belonging to Proteus spp. were collected and obtained from Department of Biology, College of Science, the University of Baghdad. The diagnosis was done by polymerase chain reaction (PCR) technique using 16S rRNA gene and urease C gene. All isolates (100%) were sensitive to meropenem, imipenem, ciprofloxacin, gentamycin, amoxicillin, clavulanic acid and levofloxacin. These isolates also showed 60% sensitivity to cefixime and nitrofurantoin. However, both species of P. mirabilis and P. vulgaris showed the lowest sensitivity when treated with tetracycline (60%) and amikacin (20%). Cephalothin had a variable effect on the species under study as P. mirabilis isolates were 100% sensitive in comparison with the 80% sensitivity of P. vulgaris isolates. The antibacterial activities of Ag and TiO 2 nanoparticles (NPs) were investigated. The minimum inhibitory concentration (MIC) value of Ag NPs against both species isolates was 10 mg/mL, while the MIC value of TiO 2 NPs was 14 mg/mL against P. mirabilis and 15 mg/mL against P. vulgaris isolates. P. mirabilis isolates showed larger swarming diameter than P. vulgaris, but this motility phenomenon of P. vulgaris was arrested rapidly after incubation with sub-MIC of TiO 2 NPs and Ag NPs comparatively with control. All isolates showed shifting to down-regulation in the fliL gene expression under the effect of the NPs using TiO 2 NPs and Ag NPs. In conclusion, down-regulation of the fliL gene expression is directly linked to the inhibition of swarming movement of Proteus species. We encourage using these inhibitors (after tests to ensure minimal toxicity to human) in combination with antibiotics to ensure bactericidal /bacteriostatic effect to treat Proteus infections.

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Flavonoid-derived anisotropic silver nanoparticles inhibit growth and change the expression of virulence genes in Escherichia coli SM10

Abstract: We hereby present a novel greener and ecofriendly synthesis of anisotropic silver nanoparticles (AgNPs) using water soluble quercetin diphosphate (QDP). QDP was employed as a reducing, capping and stabilizing agent at room temperature without any extraneous reagents.

Cited by 26 publications

References 45 publications

Green-Synthesis of Anisotropic Peptone-Silver Nanoparticles and Its Potential Application as Anti-Bacterial Agent

Green-Synthesis of Anisotropic Peptone-Silver Nanoparticles and Its Potential Application as Anti-Bacterial Agent

Size and Shape-Dependent Antimicrobial Activities of Silver and Gold Nanoparticles: A Model Study as Potential Fungicides

Down-Regulation of fliL Gene Expression by Ag Nanoparticles and TiO2 Nanoparticles in Pragmatic Clinical Isolates of Proteus mirabilis and Proteus vulgaris from Urinary Tract Infection

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