Flavonoid ofDrynaria fortunei protects against acute renal failure

Abstract

The flavonoid fraction (FF) from Drynaria fortunei was investigated to determine its biological activity expression in three acute renal failure animal models. Guinea pigs received 100 mg/kg of gentamicin (GM group), 100 mg/kg of GM plus 10 mg/kg of FF (GMFF group), 10 mg/kg of FF (FF group) or saline (saline group) intramuscularly for 14 days. The blood urea nitrogen (BUN) and creatinine levels were found to be significantly higher in the GM group (22.70+/-3.84 mg/dL, 0.68+/-0.05 mg/dL) than in the GMFF group (17.10+/-1.04 mg/dL, 0.58+/-0.09 mg/dL), the FF group (17.40+/-1.01 mg/dL, 0.49+/-0.20 mg/dL) and the saline group (17.50+/-1.22 mg/dL, 0.50+/-0.02 mg/dL). Mice were treated once with 6 mg/kg of mercuric chloride, followed by 10 mg/kg of FF or saline. On days 3, 4 and 5, BUN and creatinine levels were found to be significantly higher in the HgCl2-saline group (74.00+/-39.20 mg/dL, 59.30+/-31.20 mg/dL, 74.00+/-37.30 mg/dL and 0.53+/-0.17 mg/dL, 0.48+/-0.15 mg/dL 0.33+/-0.15 mg/dL) than in the HgCl2-FF group (19.50+/-4.90 mg/dL, 43.00+/-26.30 mg/dL, 38.50+/-13.80 mg/dL and 0.23+/-0.05 mg/dL, 0.30+/-0.12 mg/dL, 0.15+/-0.06 mg/dL).After surgery for 5/6-nephrectomy, ten mice received FF at a dose of 10 mg/kg/day and eight received saline for 42 days. The saline group survived for 12-62 days and the FF group survived for 20-320 days. The FF group had a significantly longer survival time than the saline group (p<0.05). Regeneration of kidney tubular cells and significantly enlarged convoluted tubules were noted in the pathology study of the FF group. In conclusion, the present study suggests that FF prevents nephrotoxicity, improves kidney function and promotes kidney primary epithelial tubular cell regeneration.

Antimicrobial Activity of Extract and Fractions from Drynaria fortunei Against Oral Bacteria

“…Some of the polyphenols isolated from plants exhibit anticaries activity either due to growth inhibition against mutans streptococci or due to the inhibition of glucosyltransferases (3,13). D. fortunei contains several flavonoids of which biological activities are reported as antinephrotoxicity, kidney primary epithelial tubular cell regeneration, and inhibition of gentamicin ototoxicity and bone resorption (6,9,10). Previously, researchers reported that several essential oils from plants had antibacterial activity against oral bacteria (1).…”

Antimicrobial activity of the chloroform fraction of <i>Drynaria <i>fortunei</i></i> against oral pathogens

“…The CHCl 3 extract contains naringin, hesperidin, caffeic acid, and procyanidin, but flavonoids was detected only in the n-BuOH extract for presence of organic acid (21,22,25,28,43). Some of the polyphenols isolated from plants exhibit anti-carious effects such as growth inhibition and inhibition of glucosyltransferases against mutans streptococci (44)(45)(46).…”

“…Drynaria fortunei (D. fortunei; Gol-Se-Bo in Korean and Gu-Sui-Bu in Chinese), a traditional Korean medicine, has been known to be effective in treating inflammation, hyperlipidemia, arteriosclerosis, rheumatism, gynecological diseases, osteoporosis, and bone resorption (21)(22)(23)(24)(25). It is also used for treatment and prevention of orthopedic diseases, and is known to promote bone healing (22,26).…”

New vitality of ancient ethnomedicine in China: Review of Li ethnomedicine

“…Drynaria fortunei (Kunze) J. Smith. (Long et al, 2005)); 4 are used for inflammation (e.g. Ficus pumila L.) (Junqing et al, 2009).…”