Flavonoid profile and antioxidant properties of Algerian common yew (Taxus baccata L.)

Abstract

Background: In humans, various diseases are associated with the accumulation of free radicals. The antioxidants can scavenge free radicals and reduce their impact; thus, the search for effective natural antioxidants of plant origin is indispensable. The present study aims to determine, for the first time, the flavonoid compounds profile and to investigate the free radical scavenging and antioxidant properties of the methanolic extract of Taxus baccata L. from Algeria. Methods:The determination of the flavonoid compound profile of the methanolic extract of Taxus baccata L. was established using high-performance liquid chromatography with diode-array detection coupled to electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI-MS/MS). The total flavonoid content (TFC) was performed according to the aluminum chloride colorimetric method, while the free radical scavenging and antioxidant activities were carried out using three methods, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical assay, 2,2'-azino-bis3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical assay and ferric reducing antioxidant power (FRAP) Assay.Results: A total of 26 compounds including flavon-3-ols, flavanonols, flavones, flavonols and bioflavonoids were characterized and identifiedusing HPLC-DAD-ESI-MS/MS analysis, five were reported for the first time such as taxifolin, apigenin, apigenin 7-O-glucoside, isorhamnetin 3-O-rutinoside and robustaflavone. The plant extract exhibited high total flavonoid content (TFC = 204.26 ± 6.02 mg RE/g dry extract) which corresponded to its strong radical scavenging activities [(DPPH IC 50 = 35.31 ± 0.29 µg/ml and ABTS IC 50 = 8.27 ± 0.52 µg/ml)] as compared to the synthetic antioxidant BHT [(DPPH IC 50 = 78.96 ± 5.70 µg/ml and ABTS IC 50 = 13.56 ± 0.06 µg/ml)]. However, the methanolic extract of T. baccata showed the lowest ferric reducing ability as compared to the positive controls (BHT, BHA, ascorbic acid, trolox and quercetin). Conclusion:Our results imply that the Taxus Baccata L. might be a potential source for the isolation of natural antioxidant compounds.

Metabolomic Analysis and in Vitro Investigation of the Biological Properties of a By‐Product Derived from Vicia faba

“…Several flavonoids have been proven to be powerful antioxidants that efficiently scavenge DPPH radicals due to the presence of hydroxyl groups [81,3] . In concomitant with the current findings, [82,83] evaluated the antioxidant capacity of V. faba seeds by the DPPH radical scavenging capacity, the findings revealed that immature faba beans had much higher levels of phytochemicals and demonstrated stronger antioxidant activity than mature ones [12,13] …”

“…Several flavonoids have been proven to be powerful antioxidants that efficiently scavenge DPPH radicals due to the presence of hydroxyl groups. [81,3] In concomitant with the current findings, [82,83] evaluated the antioxidant capacity of V. faba seeds by the DPPH radical scavenging capacity, the findings revealed that immature faba beans had much higher levels of phytochemicals and demonstrated stronger antioxidant activity than mature ones. [12,13] In addition, it was discovered that the methanol extract and all the investigated compounds exhibited effective inhibition for acetylcholinesterase and all carbohydrate enzymes without affecting the cell (no cytotoxic activity where the cytotoxicity showed no significant effect compared to control.…”

An In vitro and in silico investigation of the antitrypanosomal activities of the stem bark extracts of Anopyxis klaineana (Pierre) Engl

“…Having an antitrypanosomal compounds with antioxidant properties will be beneficial in condition such as trypanosomiasis, which produces ROS in response to the parasite infection [ 108 ], hence the stem bark methanolic crude extracts of A. klaineana were assessed for their antioxidant properties using FRAP and DPPH assays. These assays are simple and widely used in the estimation of antioxidant activities [ 109 , 110 ]. The DPPH assay measures the free radical scavenging activity of antioxidants.…”

In Vitro Propagation of Algerian Lavandula Stoechas and Assessment of Biochemical Composition and Their Antioxidant Activity

“…Furthermore, using a water/ethanol extract from Thymus lotocephalus in vitro cultures resulted in more phenolic compounds than those found in the original plant's leaves 51 . Plant extracts with high levels of phenolic content have a high antioxidant capacity 52 . Our findings align with a previous finding that showed a positive relationship between total phenolic and flavonoid contents and antioxidant activity in Salvia chudaei 53 .…”