Flavonoid rich extract of Murraya Koenigii alleviates in-vitro LDL oxidation and oxidized LDL induced apoptosis in raw 264.7 Murine macrophage cells

Gajaria, Tejal K.; Patel, Disha; Devkar, Ranjitsinh V.; Ramachandran, A. V.

doi:10.1007/s13197-014-1399-2

Cited by 17 publications

(19 citation statements)

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“…Cytotoxicity (MTT) assay of cells treated with cyanobacterial phycoerythrin indicated a dosedependent impact on cell viability wherein, higher dose (200 g/mL) was most potent and accounted for maximum cell growth inhibition (>80%). Transmembrane potential of mitochondria is an important parameter to assess mitochondrial function and overall health of cell Gajaria et al, 2015). In the present study, a dose-dependent decrement in mitochondrial membrane potential was recorded following cyanobacterial phycoerythrin treatment suggesting possible mitochondrial dysfunction.…”

Section: Discussionmentioning

confidence: 54%

Cyanobacterial phycoerythrin purified from marine <i>Lyngbya</i> sp. induces apoptosis in lung carcinoma cells

Madamwar

Kaushal

Patel

et al. 2015

Bangladesh J Pharmacol

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Phycoerythrins from various cyanobacterial sources have been extensively studied globally for their therapeutic potentials. This study shows the apoptotic potential of cyanobacterial phycoerythrin isolated, purified and characterized from marine Lyngbya sp. A09DM on human lung carcinoma cells (A549 cell). Results indicated that purified cyanobacterial phycoerythrin accounted for a dose-dependent decrement in cell viability, mitochondrial membrane potential and an increment in lactate dehydrogenase release. Higher dose of cyanobacterial phycoerythrin caused loss of cell viability and nuclear condensation/fragmentation. Flow cytometry accounted for more number of cells in G0/G1 phase and an increase in total number of early apoptotic cells (39.5%). Overall, this study is the first to report on apoptotic property of cyanobacterial phycoerythrin from marine Lyngbya sp. A09DM against A549 human lung carcinoma cells and requires a detailed scrutiny to establish its anti-cancer potential. Article Info

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Section: Discussionmentioning

confidence: 54%

Cyanobacterial phycoerythrin purified from marine <i>Lyngbya</i> sp. induces apoptosis in lung carcinoma cells

Madamwar

Kaushal

Patel

et al. 2015

Bangladesh J Pharmacol

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“…Gajaria et al demonstrated the protective effect of curry leaves against the in-vitro LDL-C oxidation and oxidized LDL-Cinduced apoptosis in macrophages due to the antioxidant potentials of flavonoids. 30 Birari et al reported the potent antilipase activity of curry leaves which might be involved in the decreased digestion and thus the absorption of lipids from intestine. 31 Decreased absorption of cholesterol found to increase the LDL receptor on cell surface to uptake the LDL which will lead to decrease blood cholesterol.…”

Section: Discussionmentioning

confidence: 99%

Effect of curry leaves and cucumber fruit on lipid profile in menopausal women with hyperlipidaemia: a randomized controlled pilot study

Molly

Edison²,

Vijajaraghavan

et al. 2017

Int J Clin Trials

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INTRODUCTIONCardiovascular diseases (CVD), mainly coronary heart disease (CHD) are one the largest single leading causes of morbidity and mortality all over the world. 1 Hyperlipidaemia refers to the increased levels of lipids including total cholesterol (TC) and triacyl glycerol (TAG) in blood. Although hyperlipidaemia does not cause symptoms, raised TC and TAG can significantly increase the risk of developing CHD. An adult with level more than 240 mg/dl was considered to carry twice the cardiovascular risk than those individuals at the desired level (<200 mg/dl).2 Elevated low density lipoprotein cholesterol (LDL-C) and TAG were considered detrimental of cardiovascular outcomes. 3,4 Studies from India had shown high prevalence of CHD, approximately ABSTRACT Background: Hyperlipidemia increases the risk of developing cardiovascular diseases (CVD). This study was aimed to determine the effect of curry leaves powder and slicing cucumber fruit on hyperlipidemia in the menopausal women of rural communities. Methods: Thirty menopausal healthy women of rural communities (45-65 years of age) with mild hyperlipidemia were divided and randomly assigned into control group, subjects without any treatment and 2 experimental groups, one group treated with cucumber (100-125 g) and other group treated with curry leaves powder (5 g), once daily for 45 consecutive days. The demographic variables were collected using questionnaire. Fasting blood samples were collected before and after the intervention and determined total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), high density lipoprotein-cholesterol (HDL-C) and triacyl glycerol (TAG). Results: TC, LDL-C and TAG were significantly (p <0.05) reduced in the curry leaves powder and cucumber treated groups. HDL-C level (46.1±9.2 mg/dl) was significantly (p <0.05) elevated (average 12%) only in the curry leaves powder treated group. Though the mean values of TC, LDL-C and TAG were elevated in the control group, the elevation was statistically non-significant. Comparing the efficacy of the test substances at the tested doses, HDL-C level in the curry leaves treated group showed statistically significant elevation (p =0.09). Conclusions: Curry leaves and cucumber were effective in improving HDL-C and lowering of LDL-C and TAG levels in menopausal women with hyperlipidemia, suggest the potential neutraceutical role in treating CVD.

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“…( Sánchez-Moreno et al .,2002)for example Ohmori et al, 2005),use green tea, ( Thounaojam et al .,2011). use Sida rhomboidea, (Gajaria et al, 2015 andAhmed et al, 2015) use two mint types. Our study we use NS and SL was used for inhibition of LDL oxidation in vitro, the antioxidant activity of both herbals was determined with the ability to remove free radical DPPH and with the ability to remove the reactive oxygen species (superoxide anion O2-), the EO of both herbals possess high ability to inhibit the free radical of DPPH, this finding agreed with (Hajlaoui et al,2009 andAhmed et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

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2017

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It's well documented that oxidized low density lipoprotein (LDL) plays a major role in the modification of atherosclerosis. Nigella sativa (NS) and Saussurea lappa (SL) in literature documented as a good antioxidant.To undertaken to evaluate the ability of NS and SL for inhibition of LDL oxidation in-vitro, in fact Nigella sativa and Saussurea lappa LDL was collected by ultracentrifugation and enhanced to oxidize by CuSO 4 and NS and SL were used to inhibit LDL oxidation in-vitro. Free radical scavenging of 50% of radicals of DPPH and phenolic content of both herbals were measured to assess the antioxidant activity and compared with standard antioxidant (vitamin E). The inhibitory effect of NS, SL and vitamin E against oxidation ratio was significantly better than in non addition of both herbals (p<0.01); NS was better than SL (p<0.01); and comparable with vitamin E. Both herbals possess good antioxidant activity against DPPH radicals and have high phenolic contents enable it to inhibit LDL oxidation in-vitro. NS and SL possess strong antioxidant activity comparable with vitamin E and good for cardiovascular health.

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Flavonoid rich extract of Murraya Koenigii alleviates in-vitro LDL oxidation and oxidized LDL induced apoptosis in raw 264.7 Murine macrophage cells

Cited by 17 publications

References 41 publications

Cyanobacterial phycoerythrin purified from marine <i>Lyngbya</i> sp. induces apoptosis in lung carcinoma cells

Cyanobacterial phycoerythrin purified from marine <i>Lyngbya</i> sp. induces apoptosis in lung carcinoma cells

Effect of curry leaves and cucumber fruit on lipid profile in menopausal women with hyperlipidaemia: a randomized controlled pilot study

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