Flavonoid synthesis in Lamiophlomis rotata from Qinghai-Tibet Plateau is influenced by soil properties, microbial community, and gene expression

Qian, Feng; Lu, Yueheng; Geng, Guigong; Zhou, Lianyu; Chen, Zhenning; Wang, Luhao; Xie, Hui; Qiu, Quan‐Sheng

doi:10.1016/j.jplph.2023.154043

Cited by 6 publications

(2 citation statements)

References 61 publications

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“…Consequently, species inhabiting high altitudes necessitate the adoption of multiple strategies to effectively acclimate to harsh environmental conditions. Numerous previous studies suggested that environmental factors have a significant effect on the growth and development of organisms, especially on the accumulation of their flavonoid metabolites [39][40][41]. Environmental factors contribute to the flavonoid biosynthesis of many organisms and generate economic value.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Profiling of Floccularia luteovirens from Different Geographical Regions Proposes a Novel Perspective on Their Antioxidative Activities

Tang,

Fan,

Wang

et al. 2024

Antioxidants

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Floccularia luteovirens, an endemic resource of the Tibetan Plateau, possesses significant medicinal and ecological values. However, the understanding of antioxidant capacity and metabolic profiling of F. luteovirens from diverse regions remains elusive due to limited resources. Therefore, to comprehensively comprehend the antioxidant capacity and metabolite diversity of F. luteovirens, we conducted a rounded analysis of its antioxidant capacity from three distinct regions using both untargeted and targeted metabolomics. Determination of antioxidant indices, such as ferric ion-reducing antioxidant power (FRAP), total phenolic content (TPC), and flavonoid content (FC), revealed the robust antioxidant capacity of F. luteovirens. QL F. luteovirens (QLFL) exhibited no significant difference compared to ZD F. luteovirens (ZDFL); however, both were significantly distinct from XH F. luteovirens (XHFL) across multiple indices. Furthermore, a positive correlation was observed between FRAP and flavonoid content. A total of 5782 metabolites were identified and chemically classified. Metabolites of F. luteovirens varied significantly at different regions and eight key differential metabolites were screened. Phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, and cyanoamino acid metabolism were the main different regulatory pathways. Consequently, the disparities in the antioxidant activity of F. luteovirens may primarily be ascribed to the biosynthesis and metabolism of phenylalanine, while vanillic acid could potentially serve as a pivotal metabolite influencing the antioxidative capacity of F. luteovirens by targeted metabolomics. These findings enhance our understanding of the composition of F. luteovirens and provide valuable resources for its comprehensive utilization and targeted development.

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Section: Discussionmentioning

confidence: 99%

Metabolomic Profiling of Floccularia luteovirens from Different Geographical Regions Proposes a Novel Perspective on Their Antioxidative Activities

Tang,

Fan,

Wang

et al. 2024

Antioxidants

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“…Notably, iridoid compounds such as barlerin and shanzhiside methyl ester are the most important active ingredients in P. rotata and exhibit significant anti-inflammatory and analgesic effects [ 32 , 33 , 34 ]. Currently, research on P. rotata has predominantly focused on its distribution, extraction of active ingredients, and pharmacological mechanisms [ 23 , 35 ]. However, the iridoid distribution in different regions of P. rotata leaves and the expression of genes related to the iridoid pathway remain unclear.…”

Section: Introductionmentioning

confidence: 99%

A Transcriptomic and Metabolomic Study on the Biosynthesis of Iridoids in Phlomoides rotata from the Qinghai–Tibet Plateau

Wang,

Geng,

Xie

et al. 2024

Plants

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Phlomoides rotata is a traditional Chinese herbal medicine that grows in the Qinghai–Tibet Plateau region at a 3100–5000 m altitude. Iridoid compounds are the main active compounds of the P. rotata used as medical ingredients and display anti-inflammatory, analgesic, and hepatoprotective properties. To better understand the biological mechanisms of iridoid compounds in this species, we performed a comprehensive analysis of the transcriptome and metabolome of P. rotata leaves from four different regions (3540–4270 m). Global metabolome profiling detected 575 metabolites, and 455 differentially accumulated metabolites (DAMs) were detected in P. rotata leaves from the four regions. Eight major DAMs related to iridoid metabolism in P. rotata leaves were investigated: shanzhiside methyl ester, 8-epideoxyloganic acid, barlerin, shanzhiside, geniposide, agnuside, feretoside, and catalpin. In addition, five soil physical and chemical indicators in P. rotata rhizosphere soils were analyzed. Four significant positive correlations were observed between alkaline nitrogen and geniposide, exchangeable calcium and geniposide, available potassium and shanzhiside, and available phosphorus and shanzhiside methyl ester. The transcriptome data showed 12 P. rotata cDNA libraries with 74.46 Gb of clean data, which formed 29,833 unigenes. Moreover, 78.91% of the unigenes were annotated using the eight public databases. Forty-one candidate genes representing 23 enzymes involved in the biosynthesis of iridoid compounds were identified in P. rotata leaves. Moreover, the DXS1, IDI1, 8-HGO1, and G10H2 genes associated with iridoid biosynthesis were specifically expressed in P. rotata. The integration of transcriptome and metabolome analyses highlights the crucial role of soil physical and chemical indicators and major gene expression related to iridoid metabolism pathways in P. rotata from different areas. Our findings provide a theoretical foundation for exploring the molecular mechanisms underlying iridoid compound accumulation in P. rotata.

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Seed treatment with Mortierella alpina M01 promotes tomato growth and mitigates verticillium wilt and bacterial speck disease infections by potentiating plant antioxidant responses

Nouri,

Saadaoui,

Morlevat

et al. 2024

J Plant Pathol

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Flavonoid synthesis in Lamiophlomis rotata from Qinghai-Tibet Plateau is influenced by soil properties, microbial community, and gene expression

Cited by 6 publications

References 61 publications

Metabolomic Profiling of Floccularia luteovirens from Different Geographical Regions Proposes a Novel Perspective on Their Antioxidative Activities

Metabolomic Profiling of Floccularia luteovirens from Different Geographical Regions Proposes a Novel Perspective on Their Antioxidative Activities

A Transcriptomic and Metabolomic Study on the Biosynthesis of Iridoids in Phlomoides rotata from the Qinghai–Tibet Plateau

Seed treatment with Mortierella alpina M01 promotes tomato growth and mitigates verticillium wilt and bacterial speck disease infections by potentiating plant antioxidant responses

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