Flavonoids and saponins extracted from black bean (Phaseolus vulgarisL.) seed coats modulate lipid metabolism and biliary cholesterol secretion in C57BL/6 mice

Abstract

Black bean (Phaseolus vulgaris L.) seed coats are a rich source of natural compounds with potential beneficial effects on human health. Beans exert hypolipidaemic activity; however, this effect has not been attributed to any particular component, and the underlying mechanisms of action and protein targets remain unknown. The aim of the present study was to identify and quantify primary saponins and flavonoids extracted from black bean seed coats, and to study their effects on lipid metabolism in primary rat hepatocytes and C57BL/6 mice. The methanol extract of black bean seed coats, characterised by a HPLC system with a UV-visible detector and an evaporative light-scattering detector and HPLC-time-of-flight/MS, contained quercetin 3-O-glucoside and soyasaponin Af as the primary flavonoid and saponin, respectively. The extract significantly reduced the expression of SREBP1c, FAS and HMGCR, and stimulated the expression of the reverse cholesterol transporters ABCG5/ABCG8 and CYP7A1 in the liver. In addition, there was an increase in the expression of hepatic PPAR-a. Consequently, there was a decrease in hepatic lipid depots and a significant increase in bile acid secretion. Furthermore, the ingestion of this extract modulated the proportion of lipids that was used as a substrate for energy generation. Thus, the results suggest that the extract of black bean seed coats may decrease hepatic lipogenesis and stimulate cholesterol excretion, in part, via bile acid synthesis.

Effect of flavonoid compounds extracted from Iris species in prevention of carbon tetrachloride-induced liver fibrosis in rats

“…The pharmacological functions of flavonoid compounds include multiple-treatment effects against various systematic diseases, including cardiovascular, neurological, immunological, endocrinology, and respiratory symptoms (Chavez-Santoscoy et al, 2014;Daddam et al, 2014;Sankari et al, 2014). These studies also revealed promising treatment effects of flavonoid compounds against liver damage.…”

“…Recently, promising results in the treatment of liver fibrosis have been reported using Chinese medicine (Li et al, 2014b;Zhang et al, 2014a), which often contain flavonoid compounds (Fu et al, 2014). Current studies have revealed multiple pharmacological roles of flavonoid compounds, including the treatment of tumors, cardiovascular disease, and diabetes (Chavez-Santoscoy et al, 2014;Daddam et al, 2014;Sankari et al, 2014). To further study the effect of flavonoid compounds extracted from Iris plants on liver fibrosis, we developed liver fibrosis model rats using carbon tetrachloride (CCl 4 ) and measured indices of liver function, liver fibrosis, and tissue pathological changes to evaluate their effects on CCl 4 -induced rat liver fibrosis.…”

The features of bile acids exchange in rats under the influence of corvitin

“…They found that after QUE injection the level of TBAs in rats bile was enhanced. Recently another group of resear chers reported that QUE, isolated from black beans, activated TBAs secretion in mice liver [18].…”

“…18 h of food deprivation. Every rat was anesthetized with thiopentalum natrium (Ukraine, OAO "Kyivmedpreparat", 6 mg/100 g rat b.w.).…”

Consumption of Cooked Black Beans Stimulates a Cluster of Some Clostridia Class Bacteria Decreasing Inflammatory Response and Improving Insulin Sensitivity

“…In addition, beans can provide phenolic compounds with antioxidant activity and phytosterols. Phytosterols and phenolic compounds from the black bean seed coat can potentially reduce cholesterol levels via the gut microbiota [34], by inhibiting the micellar solubility of cholesterol and reducing the expression of lipogenic genes [35,36]. A link has been suggested between the consumption of foods rich in polyphenols and the reduction of the incidence of numerous chronic disorders; however, inside the human body, the chemical structure of most polyphenols is received as xenobiotic and, therefore, the bioavailability of these compounds is greatly reduced.…”