Flavonoids and sesquiterpenoids, constituents from Eupatorium capillifolium, found in a screening study guided by cell growth inhibitory activity

Sadhu, Samir Kumar; Hirata, Kaori; Li, Xiaofan; Ohtsuki, Takashi; Koyano, Takashi; Preeprame, Srisomporn; Kowithayakorn, Thaworn; Ishibashi, Masami

doi:10.1007/s11418-006-0007-9

Cited by 6 publications

(4 citation statements)

References 22 publications

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“…5,7,4′-trihydroxy-6,3′-dimiethoxyflavone ( FL-2 ); Yellow powder; 1 H-NMR (600 MHz, CH 3 OH-d, δ = ppm): 7.43 (1H, dd, J = 8.4, 1.8 Hz, H-6′), 7.39 (1H, d, J = 1.8 Hz, H-2′), 6.90 (1H, d, J = 8.4 Hz, H-5′), 6.79 (1H, s, H-8), 6.60 (1H, s, H-3), 3.97 (3H, s, OCH 3 ), 3.82 (3H, s, OCH 3 ); 13 C-NMR (150 MHz, CH 3 OH -d, δ = ppm): 184.2, 166.8, 160.6, 154.9, 151.3, 149.1, 147.1, 133.2, 123.5, 120.4, 116.7, 114.1, 106.5, 103.8, 92.2, 61.0, 56.9; ESI-MS (positive): m / z 352.94 [M + Na] + [ 60 , 61 , 62 ].…”

Section: Methodsmentioning

confidence: 99%

Biomedical Applications of Scutellaria edelbergii Rech. f.: In Vitro and In Vivo Approach

et al. 2021

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In the current study, in vitro antimicrobial and antioxidant activities and in vivo anti-inflammatory and analgesic activities of Scutellaria edelbergii Rech. f. (crude extract and subfractions, i.e., n-hexane, ethyl acetate (EtOAc), chloroform, n-butanol (n-BuOH) and aqueous) were explored. Initially, extraction and fractionation of the selected medicinal plant were carried out, followed by phytochemical qualitative tests, which were mostly positive for all the extracts. EtOAc fraction possessed a significant amount of phenolic (79.2 ± 0.30 mg GAE/g) and flavonoid (84.0 ± 0.39 mg QE/g) content. The EtOAc fraction of S. edelbergii exhibited appreciable antibacterial activity against Gram-negative (Escherichia coli and Klebsiella pneumoniae) strains and significant zones of inhibition were observed against Gram-positive bacterial strains (Bacillus subtilis and Staphylococcus aureus). However, it was found inactive against Candida Albicans and Fusarium oxysporum fungal strains. The chloroform fraction was the most effective with an IC50 value of 172 and 74 µg/mL against DPPH (1,1-Diphenyl-2-picryl-hydrazyl) and ABTS assays, in comparison with standard ascorbic acid 59 and 63 µg/mL, respectively. Moreover, the EtOAc fraction displayed significant in vivo anti-inflammatory activity (54%) using carrageenan-induced assay and significant (55%) in vivo analgesic activity using acetic acid-induced writing assay. In addition, nine known compounds, ursolic acid (UA), ovaul (OV), oleanolic acid (OA), β-sitosterol (BS), micromeric acid (MA), taraxasterol acetate (TA), 5,3′,4′-trihydroxy-7-methoxy flavone (FL-1), 5,7,4′-trihydroxy-6,3′-dimiethoxyflavone (FL-2) and 7-methoxy catechin (FL-3), were isolated from methanolic extract of S. edelbergii. These constituents have never been obtained from this source. The structures of all the isolated constituents were elucidated by spectroscopic means. In conclusion, the EtOAc fraction and all other fractions of S. edelbergii, in general, displayed a significant role as antibacterial, free radical scavenger, anti-inflammatory and analgesic agents which may be due to the presence of these constituents and other flavonoids.

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Section: Methodsmentioning

confidence: 99%

Biomedical Applications of Scutellaria edelbergii Rech. f.: In Vitro and In Vivo Approach

et al. 2021

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“…385 The influence of the cultivation site on the guaianolide composition of Cichorium intybus has been studied. 386 Some of the lactones isolated from Eupatorium capillifolium showed potent inhibitory effects on the growth of HeLa cells, 387 whilst cynaropicrin has been shown to be a potent and irreversible inhibitor of the antibacterial target enzyme MurA. 257 The biotransformation of dehydrocostuslactone by the fungus Mucor polymorphosporus has been investigated.…”

Section: This Journal Is © the Royal Society Of Chemistry 2007mentioning

confidence: 99%

Natural Sesquiterpenoids

Fraga

2007

ChemInform

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“…A flavonoid in Artemisia argyi and Eupatorium lindleyanum [1][2][3][4], jaceosidin, also was discovered in Paronychia argentea Lam and antileishmanial metabolites from Lantana balansae [5,6]. Jaceosidin had a good anti-inflammatory effect [7][8][9][10] and played a role in immune suppression.…”

Section: Introductionmentioning

confidence: 99%

Determination and pharmacokinetic study of jaceosidin in rat plasma by UPLC–MS/MS

Zhou

Chen

et al. 2018

Acta Chromatographica

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In this work, a sensitive and selective ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and fully validated for determination of jaceosidin in rat plasma. Avicularin was used as the internal standard (IS), and protein precipitation by acetonitrile was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm) with 0.1% formic acid and acetonitrile as the mobile phase with gradient elution. An electrospray ionization (ESI) source was applied and operated in positive ion mode; multiple reaction monitoring (MRM) mode was used for quantification. Calibration plots were linear throughout the range 2-500 ng mL −1 for jaceosidin in rat plasma. Relative standard deviation (RSD) of intra-day and inter-day precision was less than 12%. The accuracy of the method was between 88.7% and 109.7%. Mean recoveries of jaceosidin in rat plasma ranged from 65.4% to 77.9%. The developed UPLC-MS/MS method was successfully applied to pharmacokinetic study of jaceosidin after intravenous administration of 2 mg kg −1 in rats. We could find that the jaceosidin rapidly eliminated, the t 1/2 was 0.7 ± 0.3 h, and clearance (CL) was 22.4 ± 3.0 L h −1 kg −1 .

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Flavonoids and sesquiterpenoids, constituents from Eupatorium capillifolium, found in a screening study guided by cell growth inhibitory activity

Cited by 6 publications

References 22 publications

Biomedical Applications of Scutellaria edelbergii Rech. f.: In Vitro and In Vivo Approach

Biomedical Applications of Scutellaria edelbergii Rech. f.: In Vitro and In Vivo Approach

Natural Sesquiterpenoids

Determination and pharmacokinetic study of jaceosidin in rat plasma by UPLC–MS/MS

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