Flavonoids as inhibitors of the formation of adenosine triphosphate in plant mitochondria

Stenlid, Göran

doi:10.1016/s0031-9422(00)85722-2

Cited by 87 publications

(27 citation statements)

References 9 publications

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“…The highest concentration of kaempferol was found on day 5 (1355 nmol/kg dry weight) after which it decreased slightly before rising once again. The residence time of kaempferol in soil remains to be investigated but possible effects of kaempferol or its derivatives on subsequent crops should not be discounted, since kaempferol has been shown to inhibit mitochondrial phosphorylation in corn, protoplasmic streaming in oat root hairs [32][33][34], and ATP formation in mitochondria of cucumber hypotyls and roots, maize coleoptiles, and pea roots [32]. Whether kaempferol autotoxicity is a factor in clover soil fatigue should be investigated.…”

Section: Fate Of Flavonoids In Soil Amended With White Clovermentioning

confidence: 99%

Fate in Soil of Flavonoids Released from White Clover (Trifolium repensL.)

Carlsen

Pedersen

Spliid

et al. 2012

Applied and Environmental Soil Science

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White clover is frequently used as a leguminous cover crop, serving as green manure, and is also included with grasses in cattle feed mixtures. Numerous biological effects reported for clover cultivation have been attributed to the production of bioactive secondary metabolites. Thus far the presence in soil of bioactive secondary metabolites from clover has received limited attention. In this paper we examine for the first time the release of flavonoids both from field-grown white clover and from soil-incorporated white clover plants of flavonoids, as analyzed by LC-MS/MS. The dominant flavonoid aglycones were formononetin, medicarpin, and kaempferol. Soil-incorporated white clover plants generated high concentrations of the glycosides kaempferol-Rha-Xyl-Gal and quercetin-Xyl-Gal. Substantial amounts of kaempferol persisted in the soil for days while the other compounds were degraded faster. These compounds should be considered in future studies of soil fatigue, allelopathic activity, and possible environmental risks from extended clover cultivation.

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Section: Fate Of Flavonoids In Soil Amended With White Clovermentioning

confidence: 99%

Fate in Soil of Flavonoids Released from White Clover (Trifolium repensL.)

Carlsen

Pedersen

Spliid

et al. 2012

Applied and Environmental Soil Science

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“…According to some authors, a negative interactions allelopathic species of the Cirsium genus (C. arvense, C. japonicum), probably due to the release by their organs phenolic compounds (Kovacs et al, 1988;Kazinczi et al, 2001;Chon et al, 2003). Phenols have inhibiting influence, among others on -ATP synthesis in mitochondria and photosynthesis (Stenlid, 1970;Johansson and Hägerby, 1974;Dadkhah, 2012). They increase the permeability of cell membranes by their depolarisation and inhibition of protein synthesis and play an important role in supplying plants with nutrients (Baziramakenga et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

The Allelopathic Potential of Cirsium oleraceum (L.) Scop. into the Fodder Meadow Plants

Barabasz‐Krasny

Możdżeń

Sołtys-Lelek

et al. 2017

Not Bot Horti Agrobo

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Due to easy vegetative and generative reproduction, the most part of the Cirsium species are considered as common weeds in different kinds of crops, on meadows and pastures. They owe their success in acquiring new area, both morphological and physiological characteristics, such as the production of allelopathic substances. An example the species within of the Cirsium genus, which may have the allelopathic properties, is cabbage thistle C. oleraceum (L.) Scop. It is a common meadow perennial plant, which in disturbed habitats quickly becomes expansive species, eliminating the other components of the flora. Such phenomenon is well-documented on the meadows of the Ojców National Park (Southern Poland). Therefore, it was investigated the effect of aqueous extracts of dried leaves C. oleraceum on seed germination, seedlings growth, the fresh and dry weight and the water content of the seedlings in three meadow species: Trifolium pratense L., Lolium multiflorum Lam. and Dactylis glomerata L. The results showed that C. oleraceum exhibits the allelopathic potential, which may have limited effect on the studied parameters of fodder species, but only when using high concentrations of extracts prepared from the leaves (15% and 20%). D. glomerata was the most sensitive on the C. oleraceum allelopathic substances.

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“…However, if synthesis of flavonoids such as kaempferol is in the chloroplasts, then there must be movement of these compounds through the cytoplasm to the vacuole. If the compounds are present as glycosides, then any effect would likely be reduced (16). Stenlid (16), speculates that since sugars are introduced rather late in the biosynthesis, it is possible to have low concentrations of the aglycones in the cytoplasm.…”

Section: Discussionmentioning

confidence: 99%

Kaempferol Inhibitions of Corn Mitochondrial Phosphorylation

Koeppe

Miller²

1974

Plant Physiol.

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Kaempferol (3,5,7,4'tetrahvdroxyflavone) inhibited the rate of state 3 substrate oxidation, but not the state 4 rate. This, along with the kaempferol inhibition of substrate-driven calcium-phosphate deposition, provided evidence that kaempferol was acting specifically on the phosphorylation mechanism and not on electron transfer. Kaempferol, however, did not inhibit ATP-driven contraction while oligomycin did. Comparisons of kaempferol with mersalyl indicated that kaempferol did not inhibit phosphorylation by blocking phosphate transport. Both kaempferol and 2, 4-dinitrophenol inhibited calcium-phosphate transport, but kaempferol did not stimulate respiration to the extent that 2, 4-dinitrophenol did under acceptorless conditions. Kaempferol had no effect on NADHdriven contraction in a potassium chloride reaction medium. The site of kaempferol effect is thus seen to be unique from oligomycin and more like aurovertin, likely acting before the formation of the phosphorylated high energy intermediate, but not as an uncoupler in the traditional 2,4-dinitrophenol mode.Flavonoid compounds have been reported to be distributed widely throughout the plant kingdom (11). Despite this wide distribution, little evidence has been presented linking their occurrence with a role in growth or metabolism. Indirect evidence suggesting a role in dormancy and in root and shoot growth has been presented by Harborne (11). It was determined that kaempferol (3,5,7,4'-tetrahydroxyflavone) was a cofactor for in vitro IAA oxidase while quercetin (3,5,7,3',4'-pentahydroxyflavone) inhibited the activity of the enzyme (6). Stenlid (16) conducted an extensive survey of the effects of many flavonoids on ATP production by mitochondria isolated from several plant species. From this work it is evident that minor changes in the hydroxyl pattern affect the degree of inhibition of ATP formation. The work of Stenlid (16) chloroplasts (20). Kaempferol glucosides were a major component of the flavonoids quantitated.Galston (7) has hypothesized from data with quercetin and kaempferol glucosides that flavonoids could play a broadly significant role in contractile and membranous phenomena in plants. Such possibilities would involve transport mechanisms associated with the production and utilization of high energy bonds, most probably as ATP and would thus suggest a possible interaction of mitochondria and flavonoids under certain conditions in vivo. In vitro mitochondrial experiments in which these interactions are studied should be useful in the furtherance of hypotheses such as Galston's (7).When the specific effects of kaempferol on mitochondrial mechanisms for oxidative phosphorylation are being considered, several sites of action are possible as evidenced in studies with other inhibitors. These include the oligomycinsensitive site in some way associated with X P (3), the DNPtsensitive site most likely associated with a proton-motive force or the nonphosphorylated intermediate (2,8,15), or the mersalyl-sensitive site associated with phosphat...

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Flavonoids as inhibitors of the formation of adenosine triphosphate in plant mitochondria

Cited by 87 publications

References 9 publications

Fate in Soil of Flavonoids Released from White Clover (Trifolium repensL.)

Fate in Soil of Flavonoids Released from White Clover (Trifolium repensL.)

The Allelopathic Potential of Cirsium oleraceum (L.) Scop. into the Fodder Meadow Plants

Kaempferol Inhibitions of Corn Mitochondrial Phosphorylation

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