Flavonoids, centaurein and centaureidin, from Bidens pilosa, stimulate IFN-γ expression

Chang, Shang‐Tzen; Chiang, Yi‐Ming; Chang, Cicero Lee Tian; Yeh, Hsu Hua; Shyur, Lie‐Fen; Kuo, Yueh Hsiung; Wu, Tung‐Kung; Yang, Wu-Chang

doi:10.1016/j.jep.2007.03.001

Cited by 83 publications

(55 citation statements)

References 21 publications

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“…These results agree with those obtained in young rats undergoing 3 weeks of cocoa consumption [14], and those obtained in OVA-immunized mice after chrysin and apigenin intake [38]. The stimulatory effect of IFNc has also been reported for other flavonoids, such as centaurein and centaureidin [39]. Since IL-4 drives Th2 differentiation [21,22] and IFNc prevents the growth of these cells [20], we can postulate that some flavonoid-rich diets, such as cocoa diets, may down-regulate Th2 immune responses by modulating cytokine production during Th polarization.…”

Section: Discussionsupporting

confidence: 90%

Influence of a cocoa‐enriched diet on specific immune response in ovalbumin‐sensitized rats

Pérez-Berezo

Ramiro-Puig

Pérez-Cano

et al. 2009

Molecular Nutrition Food Res

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Previous studies in young rats have reported the impact of 3 weeks of high cocoa intake on healthy immune status. The present article describes the effects of a longer-term cocoa-enriched diet (9 weeks) on the specific immune response to ovalbumin (OVA) in adult Wistar rats. At 4 weeks after immunization, control rats produced anti-OVA antibodies, which, according their amount and isotype, were arranged as follows: IgG1 > IgG2a > IgM > IgG2b > IgG2c. Both cocoa diets studied (4% and 10%) down-modulated OVA-specific antibody levels of IgG1 (main subclass associated with the Th2 immune response in rats), IgG2a, IgG2c and IgM isotypes. Conversely, cocoa-fed rats presented equal or higher levels of anti-OVA IgG2b antibodies (subclass linked to the Th1 response). Spleen and lymph node cells from OVA-immunized control and cocoa-fed animals proliferated similarly under OVA stimulation. However, spleen cells from cocoa-fed animals showed decreased interleukin-4 secretion (main Th2 cytokine), and lymph node cells from the same rats displayed higher interferon-gamma secretion (main Th1 cytokine). These changes were accompanied by a reduction in the number of anti-OVA IgG-secreting cells in spleen. In conclusion, cocoa diets induced attenuation of antibody synthesis that may be attributable to specific down-regulation of the Th2 immune response.

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Section: Discussionsupporting

confidence: 90%

Influence of a cocoa‐enriched diet on specific immune response in ovalbumin‐sensitized rats

Pérez-Berezo

Ramiro-Puig

Pérez-Cano

et al. 2009

Molecular Nutrition Food Res

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“…Unless otherwise stated, chemicals and reagents were purchased from Sigma (St. Louis, MO, USA), including caffeic acid phenethyl ester (CAPE), catechol, dimethyl sulfoxide (DMSO), hesperidin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lipopolysaccharide (LPS), N-[(phenylmethoxy)carbonyl]-l-leucyl-N-[(1S)-1-formyl-3-methylbutyl]-l-leucinamide (MG-132), N-(1-naphthyl)-ethylenediamine dihydrochloride, N G -monomethyl-larginine acetate salt (l-NMMA), rutin and sulfanilamide. Both, the pRL-TK plasmid containing thymidine kinase promoter linked to Renilla luciferase gene and pNF-ÄB-Luc plasmid containing a pentameric NF-ÄB enhancer binding site linked to firefly luciferase reporter gene, have been described elsewhere (Chang et al, 2007). The pGL2-iNOS-Luc plasmid harboring the 1589 bp full length iNOS promoter linked to firefly luciferase reporter gene was a kind gift from Dr. Shye-Jye Tang (Institute of Bioscience and Biotechnology, National Taiwan Ocean University).…”

Section: Plant Materials and Chemicalsmentioning

confidence: 99%

“…Cell cytotoxicity was determined using MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) colorimetric assay (Chang et al, 2007). Briefly, RAW 264.7 cells were grown in a 96-well plate containing DMEM supplemented with 10% FBS plus the crude extract, catechol, rutin, or hesperidin for 24 h. The cells were then PBS washed and 15 l MTT at 5 mg/ml was added into each well for additional 4 h incubation.…”

Section: Cell Culture and Cytotoxicity Assaymentioning

confidence: 99%

Anti-inflammatory properties of phenolic compounds and crude extract from Porphyra dentata

Kazłowska

Hsu

Hou

et al. 2010

Journal of Ethnopharmacology

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172

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“…As a folk medicine, Bp has been used in various medications. Recent studies revealed that Bp possesses various promising pharmacological effects, such as anti-allergic [8], anti-ulcer [9], anti-inflammatory [10] and immunomodulatory properties [11,12]. Particularly, Bp has attracted much interest due to its proven pharmacologic safety and its anti-inflammatory activities through inhibiting the production of pro-inflammatory cytokines.…”

Section: Introductionmentioning

confidence: 99%

The Effect of the Aqueous Extract of Bidens Pilosa L. on Androgen Deficiency Dry Eye in Rats

Zhang

Yang

et al. 2016

Cell Physiol Biochem

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Background/Aims: Bidens pilosa L. (Bp) is widely distributed in China and has been widely used as a traditional Chinese medicine. The aim of this study was to examine the effect of the extract of Bp on androgen deficiency dry eye and determine its possible mechanisms. Methods: Twenty-four rats were randomly divided into four groups: Group Con (control), Group Sal (physiological saline), Group Fin (oral finasteride), and Group Bp (oral finasteride and Bp). The dry eye model was established in group Fin and group Bp. Aqueous tear quantity was measured with phenol red-impregnated cotton threads with anesthesia. Tear film breakup time (BUT) and corneal epithelial damage were evaluated by fluorescein staining. Animals were sacrificed at 28 days, and ocular tissues (lacrimal gland and cornea) were evaluated with light microscopy; gene microarray analysis for inflammatory cytokines and Western blot were also performed. Results: Finasteride administration effectively induced dry eye in rats by 14 days after administration. Group Fin rats had significantly higher fluorescein staining scores and lower aqueous tear quantity and BUT than the group Con rats, and notable inflammatory cell infiltrates were observed in the lacrimal gland of group Fin rats. The fluorescein staining score, aqueous tear quantity and BUT significantly improved with Bp treatment in the group Bp rats, and the structures of the lacrimal gland were well maintained without significant lymphocyte infiltration. Cytokine antibody array data identified the cytokines B7-2/Cd86, IL-1β, IL-4, IL-6, IL-10, MMP-8, FasL, TNF-α and TIMP-1 as candidates for validation by Western blot. Expression levels of pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α, in group Fin were upregulated compared with group Con. Levels of anti-inflammatory cytokines, such as IL-4 and IL-10, in group Fin were also upregulated compared with those in group Con. Compared with group Fin, IL-1β, FasL, and TNF-α were significantly decreased in group Bp. Conclusion: The extract of Bp appears to be effective for the treatment of androgen deficiency dry eye in rats by improving aqueous tear quantity, maintaining tear film stability, and inhibiting the inflammation of the lacrimal gland.

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Flavonoids, centaurein and centaureidin, from Bidens pilosa, stimulate IFN-γ expression

Cited by 83 publications

References 21 publications

Influence of a cocoa‐enriched diet on specific immune response in ovalbumin‐sensitized rats

Influence of a cocoa‐enriched diet on specific immune response in ovalbumin‐sensitized rats

Anti-inflammatory properties of phenolic compounds and crude extract from Porphyra dentata

The Effect of the Aqueous Extract of Bidens Pilosa L. on Androgen Deficiency Dry Eye in Rats

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