Flavonoids extracted from mulberry (Morus alba L.) leaf improve skeletal muscle mitochondrial function by activating AMPK in type 2 diabetes

Meng, Qingcai; Xu, Qi; Fu, Yu; Chen, Qi; Peng, Cheng; Yu, Xichao; Sun, Xin; Wu, Jian; Li, Wenwen; Zhang, Qichun; Yu, Li; Wang, Aiyun; Bian, Huimin

doi:10.1016/j.jep.2019.112326

Cited by 106 publications

(65 citation statements)

References 37 publications

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“…Furthermore, activation of adipose tissues can enhance thermogenesis and energy expenditure leading to improved glucose homeostasis and insulin sensitivity (31,32). Moreover, MLF have shown to trigger the AMPK-PGC-1α signaling pathway to improve glucose metabolism and enhance mitochondrial function to restore ATP homeostasis in skeletal muscles (33). No effect of MLF on respiratory rate and body surface temperature shows the absence of any adverse effects of MLF on the physiology of buffaloes.…”

Section: Physiological and Serum Biochemical Parametersmentioning

confidence: 99%

Mulberry Leaf Flavonoids Improve Milk Production, Antioxidant, and Metabolic Status of Water Buffaloes

Hassan

Tang

et al. 2020

Front. Vet. Sci.

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Section: Physiological and Serum Biochemical Parametersmentioning

confidence: 99%

Mulberry Leaf Flavonoids Improve Milk Production, Antioxidant, and Metabolic Status of Water Buffaloes

Hassan

Tang

et al. 2020

Front. Vet. Sci.

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“…The main ingredient of rutin in mulberry leaf extracts flavonoids (1 g/mL) were determined using an Agilent 1260 liquid chromatography system. The procedure of HPLC chromatograms analysis of flavonoids that extracts from mulberry leaves could been seen in our previous studied [10]. Here we provided a representative HPLC chromatograms of flavonoids that extracts from mulberry leaves, it can be seen in Fig.…”

Section: Methodsmentioning

confidence: 99%

“…The mulberry leaves were provided by the Beijing Tong Ren Tang Co. Ltd (Beijing, China). The flavonoids extract of mulberry leaves was prepared with the following procedure that we have reported before [10]. The main ingredient of rutin in mulberry leaf extracts flavonoids (1 g/mL) were determined using an Agilent 1260 liquid chromatography system.…”

Section: Methodsmentioning

confidence: 99%

IRS1/PI3K/AKT pathway signal involved in the regulation of glycolipid metabolic abnormalities by Mulberry (Morus alba L.) leaf extracts in 3T3-L1 adipocytes

Meng

Cao

et al. 2020

Chin Med

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Background: Mulberry (Morus alba L.) leaf tea benefits the control of diabetes in Asian nations. This study was aim to investigate if the flavonoids, which extracts from mulberry leaves, could regulate the metabolism of glycolipid, and to investigate if flavonoids could regulate IRS1/PI3K/AKT pathway signal to affect the expression of FAS and membrane transfer capacity GLUT4 in 3T3-L1 adipocytes. Results: Results revealed that flavonoids decreased the levels of free fatty acid and increased the glucose consumption and the levels of adiponectin and leptin in a dose-dependent manner, and remarkably increased the protein expression levels of p-IRS1, p-PI3K, p-Akt, total GLUT4, and membrane GLUT4, and decreased the protein expression levels of PTEN and FAS in 3T3-L1 adipocytes IR model. On the other hand, wortmannin (2 nM), a selective and irreversible PI3K inhibitor, significantly decreased the glucose consumption and the adiponectin and leptin levels, and increased the free fatty acid level in flavonoids treated 3T3-L1 adipocytes IR model. Furthermore, wortmannin (2 nM) partly eliminated the activation of PI3K/AKT signaling, the suppression of FAS, and the up-regulated membrane transfer capacity of GLUT4 in flavonoids treated 3T3-L1 adipocytes IR model. Conclusion: In conclusion, our results illustrated that mulberry leaf extracts flavonoids alleviated the glycolipid metabolic abnormalities in 3T3-L1 adipocytes IR model, and the effect was associated with the activation of IRS1/PI3K/AKT pathway, the suppression of FAS, and the up-regulation of membrane transfer capacity of GLUT4.

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“…Recently, mulberry leaves (MLs) have been recognized as edible with medicinal properties owing to their phytochemical content [4]. Phytochemical studies have shown that MLs are a rich source of a variety of constituents, including flavonoids [3,5], polysaccharides [1,6], alkaloids [7], and 1-deoxynojirimycin [8]. Besides, several different flavonol (i.e., quercetin and kaempferol) glycosides have been reported as antioxidant constituents in MLs.…”

Section: Introductionmentioning

confidence: 99%

“…These active components contain reactive oxygen species (ROS) scavengers [4,9,10]. Substantial previous evidences indicated that MLs could be a potential functional ingredient for the production of healthy foods [5][6][7]. However, the leaves of black mulberry varieties are generally discarded because they are planted only for fruit, and the leaves are not advisable for use as food even for silkworms [11].…”

Section: Introductionmentioning

confidence: 99%

Response Surface Methodology for Optimization of Process Parameters and Antioxidant Properties of Mulberry (Morus alba L.) Leaves by Extrusion

Kim

Nam

et al. 2020

Molecules

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Mulberry (Morus alba L.) leaves (MLs), originally used to feed silkworms, have recently been recognized as a food ingredient containing health-beneficial, bioactive compounds. In this study, the extrusion process was applied for the enhancement of the amount of extractable flavonoids from MLs. Extrusion conditions were optimized by water solubility index, total phenolic content, and total flavonoid content (TF) using response surface methodology, and antioxidative stress activities were evaluated in macrophage cells. According to the significance of regression coefficients of TF, the optimal extrusion parameters were set as barrel temperature of 114 °C, moisture feed content of 20%, and screw speed of 232 rpm. Under these conditions, the TF of extruded ML reached to 0.91% and improved by 63% compared with raw ML. Fifteen flavonoids were analyzed using ultra-high-performance liquid chromatograph coupled with photodiode array detection and quadrupole time-of-flight mass spectrometry (UPLC-PDA-QTOF/MS), and the extrusion resulted in increases in quercetin-3-gentiobioside, quercetin-3,7-di-O-glucoside, kaempferol-3,7-di-O-glucoside, rutin, isoquercitrin, and moragrol C. Besides, regarding antioxidative activity, extruded ML water extract inhibited the production of H2O2-induced reactive oxygen species and attenuated nuclear morphology alterations in macrophage cells. The findings of this study should be useful in food processing design to improve the extractable functional compounds in MLs.

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Flavonoids extracted from mulberry (Morus alba L.) leaf improve skeletal muscle mitochondrial function by activating AMPK in type 2 diabetes

Cited by 106 publications

References 37 publications

Mulberry Leaf Flavonoids Improve Milk Production, Antioxidant, and Metabolic Status of Water Buffaloes

Mulberry Leaf Flavonoids Improve Milk Production, Antioxidant, and Metabolic Status of Water Buffaloes

IRS1/PI3K/AKT pathway signal involved in the regulation of glycolipid metabolic abnormalities by Mulberry (Morus alba L.) leaf extracts in 3T3-L1 adipocytes

Response Surface Methodology for Optimization of Process Parameters and Antioxidant Properties of Mulberry (Morus alba L.) Leaves by Extrusion

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