Flavonoids furom Coreopsis tinctoria adjust lipid metabolism in hyperlipidemia animals by down-regulating adipose differentiation-related protein

Abstract

BackgroundTo identify the chemical structure of Coreopsis tinctoria extracts and their effect and mechanism on reducing blood lipid in hyperlipemia mice.MethodsThe flavonoids were extracted from Coreopsis tinctoria. The chemical structure was identified by HPLC. 59 mice were divided randomly into 5 groups. (group 1: normal diet control; group 2: hyperlipemia model; group 3: hyperlipemia mice treated with Coreopsis tinctoria, low dose 100 mg/kg; group 4: hyperlipemia mice treated with Coreopsis tinctoria high dose group 200 mg/kg; group 5 hyperlipemia mice treated with Fenofibrate. After 2 week of hyperlipid diet, the treatment of Coreopsis tinctoria and Fenofibrate were given for another 6 weeks with continuous hyperlipid diet. The TC, TG, HDL, histology, adipose differentiation-related protein (ADRP) expression in different groups were compared.ResultsCompared with normal diet group, TC, TG in hyperlipemia model group increased ( P < 0. 01). After treatment with Coreopsis tinctoria low dose group, high dose group, TC of the hyperlipemia mice decreased (P < 0. 05) without increasing AST, ALT and ALP. Fenofibrate can also decrease TC and TG but increase AST, ALT and ALP. Expression of hepatic ADRP increased in hyperlipemia mice. Coreopsis tinctoria high dose group 200 mg/kg can inhibit ADRP as Fenofibrate does.ConclusionThe flavonoids from Coreopsis tinctoria extracts can reduce blood lipid without liver function damage, showing better anti- hyperlipemia effect than Fenofibrate by down-regulating ADRP.

Inflammation and Oxidative Stress in Chronic Kidney Disease—Potential Therapeutic Role of Minerals, Vitamins and Plant-Derived Metabolites

“…C. tinctoria is an ornamental plant widely cultivated in China and in many other counties, such as Canada, Mexico and the United States. It has been reported that C. tinctoria possesses many biological activities, including anti-inflammatory, antioxidant, anti-hyperlipidemic and anti-hypoglycemic activities [156,157]. Its anti-inflammatory activity has been mainly attributed to inhibition of COX-2 expression [158].…”

Coreopsis Tinctoria Modulates Lipid Metabolism by Decreasing Low-Density Lipoprotein and Improving Gut Microbiota

“…In our previous research using an animal model, we found the active flavonoid compounds from CT extracts have anti-hyperlipidemia effects, particularly in lowering triglycerides, reducing lipid deposition, and protecting liver function [9]. Reductions in LDL can decrease major cardiovascular events in clinical trials [31,32]; thus, reducing LDL cholesterol has been incorporated into practice guidelines as a fundamental means of reducing cardiovascular morbidity and mortality [33,34].…”

“…The mouse model of hyperlipidemia was generated by oral feeding of a high-fat diet (20% lard, 10% cholesterol, 2% bile salts, 0.2% propylthiouracil, 20% propylene glycol, 20% polysorbate-80; 0.2 mg/per 10 g weight per day) for 2 weeks [9]. The hyperlipidemia model was confirmed by blood lipid tests after continuous feeding for 2 weeks.…”

“…In our previous study, we identified the chemical composition of CT extracts using a high-performance liquid chromatography/photodiode array system [9]. The constituent compounds are mainly aglycones and glycosides of the flavanone/chalcone flavonoid type.…”

“…Because its growth environment is less polluted and occurs at high altitudes, CT contains many kinds of compounds that benefit human health, and has gradually become popular as a type of tea drink. Our previous study indicated that the flavonoids extracted from CT show anti-hyperlipidemic effects, particularly in lowering triglycerides, reducing lipid deposition, and protecting liver function in an animal model [9]. Some pharmacological studies have also reported that CT is rich in flavonoids and can adjust glucose metabolism, promote blood circulation, and reduce cardiovascular disease risk [10,11].…”