Flavonoids Isolated from KoreaCitrus aurantiumL. Induce G2/M Phase Arrest and Apoptosis in Human Gastric Cancer AGS Cells

Abstract

Aim of the Study. Citrus species is used in traditional medicine as medicinal herb in several Asian countries including Korea. Flavonioids became known as various properties, such as anti-oxidants, anti-inflammation and anti-cancer, and so forth. The present study, the anti-cancer effect of flavonioids isolated from Citrus aurantium L. in human gastric cancer AGS cells has been investigated. Materials and Methods. The anti-proliferative activity was assayed using MTT assay. Cell cycle analysis was done using flow cytometry and apoptosis detection was done using by hoechst fluorescent staining and Annexin V-propidium iodide double staining. Western blot was used to detect the expression of protein related with cell cycle and apoptosis. Results. Flavonoids isolated from Citrus aurantium L. have the effect of anti proliferation on AGS cells with IC50 value of 99 μg/mL. Flavonoids inhibited cell cycle progression in the G2/M phase and decrease expression level of cyclin B1, cdc 2, cdc 25c. Flavonoids induced apoptosis through activate caspase and inactivate PARP. Conclusions. Flavonoids isolated from Citrus aurantium L. induced G2/M phase arrest through the modulation of cell cycle related proteins and apoptosis through activation caspase. These finding suggest flavonoids isolated from Citrus aurantium L. were useful agent for the chemoprevention of gastric cancer.

Hesperidin Induces Paraptosis Like Cell Death in Hepatoblatoma, HepG2 Cells: Involvement of ERK1/2 MAPK

“…In our previous studies we have shown high content of hesperidin in C. aurantium [16]. In food industry Citrus fruits are mainly used as fresh juice and Citrus based juice.…”

“…Natural products are considered to be safe and also reduce the mutagenicity in normal cells [19]. In our previous studies we have also reported Citrus aurantium extracts, Scutellaria baccalensis extracts and polyphenolic extracts of Lonicera japonica to possess anticancer activity in human gastric cancer, lung cancer and liver cancer cells respectively [16], [26], [27].…”

Citrus aurantium flavonoids inhibit adipogenesis through the Akt signaling pathway in 3T3-L1 cells

“…CAL consists of many flavonoids, naringin, hesperidin, poncirin, isosiennsetin, hexamethoxyflavone, sineesytin, hexamethoxyflavone, tetramrthnl-o-isoscutellaeein, nobiletin, heptamethoxyflavone, 3-hydoxynobiletin, tangeretin, hydroxypentamethoxyflavone, and hexamethoxyflavone [22,23]. …”

Anticancer effects of traditional Chinese herbs with phlegm-eliminating properties – An overview

“…With the effect on circulating qi, drying dampness and transforming phlegm, it is used to treat cough and cancer in TCM practice. Current studies have indicated that the extracts or constituents of C. reticulata Blanco have anticancer effects on several tumor cell lines (Silalahi, 2002;Arias and Ramon-Laca, 2005), and its mechanism involving induction of apoptosis was demonstrated on human leukemic HL-60 (Hirano et al, 1995), gastric cancer (Kim et al, 2005;Lee et al, 2012b), colorectal cancer (Zheng et al, 2002), and lung cancer cells (Xiao et al, 2009). It is believed that the main active ingredients of C. reticulata Blanco are flavonoids, particularly polymethoxyflavones (PMFs), which are reported to have a role in inducing an increased level of intracellular Ca 2 þ and thus activating Ca 2 þ -dependent apoptotic proteases, and modulating key signaling proteins related to cell proliferation and apoptosis (Sergeev et al, 2007;Qiu et al, 2010).…”