Flavonoids ofPseudosophora alopecuroides

Abstract

UDC 547.972Pseudosophora alopecuroides (L.) Sweet (ram. Fabaceae) is a perennial herbaceous weed plant possessing an insecticidal and repellent action [1, 2]. In folk medicine, crushed seeds of this plant are recommended for poor digestion and loss of appetite [2]. The flavonoids isobavachin, glabrol, ammothamnidin, trifolirhizin, vexibinol, and vexibidin have previously been isolated from its roots [3, 4].We have now studied the flavonoids of the epigeal part of the plant gathered in the fruit-bearing period on the territory of the Republic of Karakalpakstan.The air-dry comminuted raw material (2.7 kg) was extracted with chloroform (5 x 10 liters) at room temperature. The chloroform was distilled off, the residue was dissolved in hot ethanol, the solution was diluted with water, and the resulting precipitate was filtered off. The filtrate was evaporated in vacuum, and the residue was dried and chromatographed on a column of silica gel in a chloroform-hexane gradient system and chloroform. Compounds (1-4) were obtained.By the column chromatography on siliea gel of an alcoholic extract of roots gathered in the Tashkent oblast we have isolated vexi~binol, trifolirhizin, and substance (5).Compounds (1-5) were identified from khe results of spectral (UV, mass, and 1H and 13C NMR) investigations and, in the case of substances (1-4), by direct comparison with authentic specimens.

High-Speed Counter-Current Chromatography with an Online Storage Technique for the Preparative Isolation and Purification of Dihydroflavonoids from Sophora alopecuroides L.

“…However, flavonoids are abundant in the roots of S. alopecuroides and show significant antibacterial activities (Wan et al , ). The separation methods of flavonoids reported use conventional silica column chromatography, preparative HPLC and LH‐20 gel (Wan et al , ; Butayarov et al , ; Iinuma et al , ). These methods have the disadvantages of a small preparative capacity and serious loss of absorbance for the components.…”